LOCUS       BBa_K223010             1681 bp    DNA     linear       22-Nov-2024
DEFINITION  RBS + SoxR + TT + SoxS + RBS + BLH + TT
ACCESSION   BBa_K223010
VERSION     BBa_K223010.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..483
                     /label=BBa_K223000
     stem_loop       503..546
                     /label=stem_loop
     misc_feature    492..571
                     /label=BBa_B0010
     terminator      492..571
                     /label=BBa_B0010
     stem_loop       587..606
                     /label=T7 TE
     misc_feature    613..613
                     /label=stop
     polyA_site      607..620
                     /label=polya
     misc_feature    580..620
                     /label=BBa_B0012
     terminator      580..620
                     /label=BBa_B0012
     promoter        629..690
                     /label=BBa_K223001
     misc_feature    703..706
                     /label=conserved
     RBS             699..710
                     /label=BBa_B0034
     stem_loop       1564..1607
                     /label=stem_loop
     misc_feature    1553..1632
                     /label=BBa_B0010
     terminator      1553..1632
                     /label=BBa_B0010
     stem_loop       1648..1667
                     /label=T7 TE
     misc_feature    1674..1674
                     /label=stop
     polyA_site      1668..1681
                     /label=polya
     misc_feature    1641..1681
                     /label=BBa_B0012
     terminator      1641..1681
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggaaaagaaa ttaccccgca ttaaagcgct gctaaccccc
       61 ggcgaagtgg cgaaacgcag cggtgtggcg gtatcggcgc tgcatttcta tgaaagtaaa
      121 gggttgatta ccagtatccg taacagcggc aatcagcggc gatataaacg tgatgtgttg
      181 cgatatgttg caattatcaa aattgctcag cgtattggca ttccgctggc gaccattggt
      241 gaagcgtttg gcgtgttgcc cgaagggcat acgttaagtg cgaaagagtg gaaacagctt
      301 tcgtcccaat ggcgagaaga gttggatcgg cgcattcata ccttagtggc gctgcgtgac
      361 gaactggacg gatgtattgg ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac
      421 ccgggcgacc gcttaggaga agaaggtacc ggcgcacgct tgctggaaga tgaacaaaac
      481 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      541 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      601 tgggcctttc tgcgtttata tactagagaa tcgctttacc tcaagttaac ttgaggaatt
      661 atactcccca acagatgaat taacgaactg tactagagaa agaggagaaa tactagatgg
      721 gtctgatgct gattgattgg tgtgcactgg ctctggttgt tttcattggc ctgccgcacg
      781 gcgcgctgga tgctgccatt tctttttcta tgatctcttc tgcaaaacgc attgctcgtc
      841 tggctggtat tctgctgatc tatctgctgc tggcgaccgc gttcttcctg atctggtatc
      901 agctgccagc gtttagcctg ctgatcttcc tgctgatctc cattatccac tttggtatgg
      961 cagacttcaa cgcgtcccca agcaaactga aatggccgca tatcatcgcc cacggcggtg
     1021 ttgttactgt ttggctgccg ctgatccaga aaaacgaagt aactaaactg tttagcatcc
     1081 tgactaacgg tccgactccg atcctgtggg acatcctgct gattttcttc ctgtgttggt
     1141 ctattggcgt gtgtctgcac acgtacgaaa ccctgcgctc taaacattac aacatcgcct
     1201 ttgaactgat cggtctgatt ttcctggcgt ggtatgcgcc gcctctggtt acgtttgcca
     1261 cttacttctg cttcattcat tcccgtcgcc acttctcctt tgtgtggaag cagctgcaac
     1321 acatgtcttc caaaaagatg atgattggca gcgcgattat cctgtcctgt acctcttggc
     1381 tgatcggcgg tggtatctat ttcttcctga actccaaaat gatcgcctct gaggctgcgc
     1441 tacaaactgt gttcatcggt ctggcggcac tgaccgtgcc gcacatgatt ctgatcgact
     1501 tcatcttccg tccgcactct tcccgtatca aaatcaaaaa ctgatactag agccaggcat
     1561 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1621 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1681 a
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