LOCUS       BBa_K223011             3243 bp    DNA     linear       16-Jun-2024
DEFINITION  RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT
ACCESSION   BBa_K223011
VERSION     BBa_K223011.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..483
                     /label=BBa_K223000
     stem_loop       503..546
                     /label=stem_loop
     misc_feature    492..571
                     /label=BBa_B0010
     terminator      492..571
                     /label=BBa_B0010
     stem_loop       587..606
                     /label=T7 TE
     misc_feature    613..613
                     /label=stop
     polyA_site      607..620
                     /label=polya
     misc_feature    580..620
                     /label=BBa_B0012
     terminator      580..620
                     /label=BBa_B0012
     promoter        629..690
                     /label=BBa_K223001
     misc_feature    703..706
                     /label=conserved
     RBS             699..710
                     /label=BBa_B0034
     misc_feature    1557..1560
                     /label=conserved
     RBS             1553..1564
                     /label=BBa_B0034
     stem_loop       3126..3169
                     /label=stem_loop
     misc_feature    3115..3194
                     /label=BBa_B0010
     terminator      3115..3194
                     /label=BBa_B0010
     stem_loop       3210..3229
                     /label=T7 TE
     misc_feature    3236..3236
                     /label=stop
     polyA_site      3230..3243
                     /label=polya
     misc_feature    3203..3243
                     /label=BBa_B0012
     terminator      3203..3243
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggaaaagaaa ttaccccgca ttaaagcgct gctaaccccc
       61 ggcgaagtgg cgaaacgcag cggtgtggcg gtatcggcgc tgcatttcta tgaaagtaaa
      121 gggttgatta ccagtatccg taacagcggc aatcagcggc gatataaacg tgatgtgttg
      181 cgatatgttg caattatcaa aattgctcag cgtattggca ttccgctggc gaccattggt
      241 gaagcgtttg gcgtgttgcc cgaagggcat acgttaagtg cgaaagagtg gaaacagctt
      301 tcgtcccaat ggcgagaaga gttggatcgg cgcattcata ccttagtggc gctgcgtgac
      361 gaactggacg gatgtattgg ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac
      421 ccgggcgacc gcttaggaga agaaggtacc ggcgcacgct tgctggaaga tgaacaaaac
      481 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      541 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      601 tgggcctttc tgcgtttata tactagagaa tcgctttacc tcaagttaac ttgaggaatt
      661 atactcccca acagatgaat taacgaactg tactagagaa agaggagaaa tactagatgg
      721 gtctgatgct gattgattgg tgtgcactgg ctctggttgt tttcattggc ctgccgcacg
      781 gcgcgctgga tgctgccatt tctttttcta tgatctcttc tgcaaaacgc attgctcgtc
      841 tggctggtat tctgctgatc tatctgctgc tggcgaccgc gttcttcctg atctggtatc
      901 agctgccagc gtttagcctg ctgatcttcc tgctgatctc cattatccac tttggtatgg
      961 cagacttcaa cgcgtcccca agcaaactga aatggccgca tatcatcgcc cacggcggtg
     1021 ttgttactgt ttggctgccg ctgatccaga aaaacgaagt aactaaactg tttagcatcc
     1081 tgactaacgg tccgactccg atcctgtggg acatcctgct gattttcttc ctgtgttggt
     1141 ctattggcgt gtgtctgcac acgtacgaaa ccctgcgctc taaacattac aacatcgcct
     1201 ttgaactgat cggtctgatt ttcctggcgt ggtatgcgcc gcctctggtt acgtttgcca
     1261 cttacttctg cttcattcat tcccgtcgcc acttctcctt tgtgtggaag cagctgcaac
     1321 acatgtcttc caaaaagatg atgattggca gcgcgattat cctgtcctgt acctcttggc
     1381 tgatcggcgg tggtatctat ttcttcctga actccaaaat gatcgcctct gaggctgcgc
     1441 tacaaactgt gttcatcggt ctggcggcac tgaccgtgcc gcacatgatt ctgatcgact
     1501 tcatcttccg tccgcactct tcccgtatca aaatcaaaaa ctgatactag agaaagagga
     1561 gaaatactag atgcccggcg aggtgaaggc cgaccccgcc gcgctcatgg cctcgctgca
     1621 gctcctgccg tcacccacgc ccaacctcga gatcaagtac accaagatct ttattaacaa
     1681 tgaatggcag aactcagaga gtgggagagt gttccctgtc tgtaatccag ccacaggaga
     1741 gcaagtgtgt gaagttcaag aagcagacaa ggtggatata gacaaggcag tgcaggcggc
     1801 ccgcctggcc ttctctcttg gttctgtgtg gagaaggatg gacgcttctg aaagaggacg
     1861 tctgctggac aagcttgcag acttggtgga acgggacagg gcaactcttg caactatgga
     1921 atcactaaat ggcggtaagc cattcctgca agctttttac atcgatttgc agggagtcat
     1981 caaaaccctg aggtattatg caggctgggc tgataaaatt cacggaatga ccattcctgt
     2041 agatggagac tattttacct tcacgagaca tgagcccatc ggagtgtgtg gacagattat
     2101 cccgtggaat ttccccctgc tgatgttcac ctggaaaatt gctcccgcac tgtgctgtgg
     2161 taacactgtg gtcatcaagc ctgcagagca gacaccactc agtgcactgt atatgggagc
     2221 cctcatcaag gaggctggct ttccacccgg ggtcgtcaac attctgccag ggtatgggcc
     2281 aacagcaggg gcagcaatag cgtctcacat tggcatagac aagattgcat tcacaggctc
     2341 tactgaggtt ggaaagctta ttcaagaagc agctggaaga agtaatttga agagagtaac
     2401 tctggaactt ggagggaaaa gccctaatat tatttttgca gatgctgact tggactacgc
     2461 tgtggagcag gctcaccagg gtgtgttctt caaccaaggc cagtgctgca ctgcggggtc
     2521 tcgaatcttt gtggaggagt ccatctatga ggaatttgtg aaaagaagtg tggaaagagc
     2581 caagaggcgc attgtgggga gcccctttga tcccactact gagcagggtc ctcagattga
     2641 taagaagcaa tacaacaaaa tcctggagct catccagagc ggtgtggccg agggcgccaa
     2701 gctcgagtgt ggaggcaaag gactgggaag gaagggcttc ttcattgagc ctaccgtgtt
     2761 ctccaacgtt acagatgaca tgcggatcgc caaggaggag atctttggtc ctgtccagga
     2821 aattttgagg tttaagacta tggatgaagt tatagaaaga gccaataact cagacttcgg
     2881 gcttgtagcg gctgtcttca ctaatgacat caacaaggct ctcatggtgt cctctgcaat
     2941 gcaagctggg acagtttgga tcaattgtta caacgcctta aatgctcaga gccccttcgg
     3001 ggggttcaag atgtctggaa atgggagaga aatgggtgag tttggcttac gggagtactc
     3061 agaagtaaag accgtgacgg tgaagatccc ccagaagaac tcctaatact agagccaggc
     3121 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     3181 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     3241 ata
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