LOCUS       BBa_K223012             1724 bp    DNA     linear       05-Nov-2024
DEFINITION  SCP + RBS + SoxR + TT + SoxS + RBS + BLH + TT
ACCESSION   BBa_K223012
VERSION     BBa_K223012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..526
                     /label=BBa_K223000
     stem_loop       546..589
                     /label=stem_loop
     misc_feature    535..614
                     /label=BBa_B0010
     terminator      535..614
                     /label=BBa_B0010
     stem_loop       630..649
                     /label=T7 TE
     misc_feature    656..656
                     /label=stop
     polyA_site      650..663
                     /label=polya
     misc_feature    623..663
                     /label=BBa_B0012
     terminator      623..663
                     /label=BBa_B0012
     promoter        672..733
                     /label=BBa_K223001
     misc_feature    746..749
                     /label=conserved
     RBS             742..753
                     /label=BBa_B0034
     stem_loop       1607..1650
                     /label=stem_loop
     misc_feature    1596..1675
                     /label=BBa_B0010
     terminator      1596..1675
                     /label=BBa_B0010
     stem_loop       1691..1710
                     /label=T7 TE
     misc_feature    1717..1717
                     /label=stop
     polyA_site      1711..1724
                     /label=polya
     misc_feature    1684..1724
                     /label=BBa_B0012
     terminator      1684..1724
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggaaaag aaattacccc gcattaaagc gctgctaacc cccggcgaag tggcgaaacg
      121 cagcggtgtg gcggtatcgg cgctgcattt ctatgaaagt aaagggttga ttaccagtat
      181 ccgtaacagc ggcaatcagc ggcgatataa acgtgatgtg ttgcgatatg ttgcaattat
      241 caaaattgct cagcgtattg gcattccgct ggcgaccatt ggtgaagcgt ttggcgtgtt
      301 gcccgaaggg catacgttaa gtgcgaaaga gtggaaacag ctttcgtccc aatggcgaga
      361 agagttggat cggcgcattc ataccttagt ggcgctgcgt gacgaactgg acggatgtat
      421 tggttgtggc tgcctttcgc gcagtgattg cccgttgcgt aacccgggcg accgcttagg
      481 agaagaaggt accggcgcac gcttgctgga agatgaacaa aactaatact agagccaggc
      541 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      601 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      661 atatactaga gaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg
      721 aattaacgaa ctgtactaga gaaagaggag aaatactaga tgggtctgat gctgattgat
      781 tggtgtgcac tggctctggt tgttttcatt ggcctgccgc acggcgcgct ggatgctgcc
      841 atttcttttt ctatgatctc ttctgcaaaa cgcattgctc gtctggctgg tattctgctg
      901 atctatctgc tgctggcgac cgcgttcttc ctgatctggt atcagctgcc agcgtttagc
      961 ctgctgatct tcctgctgat ctccattatc cactttggta tggcagactt caacgcgtcc
     1021 ccaagcaaac tgaaatggcc gcatatcatc gcccacggcg gtgttgttac tgtttggctg
     1081 ccgctgatcc agaaaaacga agtaactaaa ctgtttagca tcctgactaa cggtccgact
     1141 ccgatcctgt gggacatcct gctgattttc ttcctgtgtt ggtctattgg cgtgtgtctg
     1201 cacacgtacg aaaccctgcg ctctaaacat tacaacatcg cctttgaact gatcggtctg
     1261 attttcctgg cgtggtatgc gccgcctctg gttacgtttg ccacttactt ctgcttcatt
     1321 cattcccgtc gccacttctc ctttgtgtgg aagcagctgc aacacatgtc ttccaaaaag
     1381 atgatgattg gcagcgcgat tatcctgtcc tgtacctctt ggctgatcgg cggtggtatc
     1441 tatttcttcc tgaactccaa aatgatcgcc tctgaggctg cgctacaaac tgtgttcatc
     1501 ggtctggcgg cactgaccgt gccgcacatg attctgatcg acttcatctt ccgtccgcac
     1561 tcttcccgta tcaaaatcaa aaactgatac tagagccagg catcaaataa aacgaaaggc
     1621 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1681 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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