LOCUS       BBa_K223016             2432 bp    DNA     linear       05-Nov-2024
DEFINITION  MCP + RBS + SoxR + TT + SoxS + RBS + RalDH II + TT
ACCESSION   BBa_K223016
VERSION     BBa_K223016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..526
                     /label=BBa_K223000
     stem_loop       546..589
                     /label=stem_loop
     misc_feature    535..614
                     /label=BBa_B0010
     terminator      535..614
                     /label=BBa_B0010
     stem_loop       630..649
                     /label=T7 TE
     misc_feature    656..656
                     /label=stop
     polyA_site      650..663
                     /label=polya
     misc_feature    623..663
                     /label=BBa_B0012
     terminator      623..663
                     /label=BBa_B0012
     promoter        672..733
                     /label=BBa_K223001
     misc_feature    746..749
                     /label=conserved
     RBS             742..753
                     /label=BBa_B0034
     stem_loop       2315..2358
                     /label=stem_loop
     misc_feature    2304..2383
                     /label=BBa_B0010
     terminator      2304..2383
                     /label=BBa_B0010
     stem_loop       2399..2418
                     /label=T7 TE
     misc_feature    2425..2425
                     /label=stop
     polyA_site      2419..2432
                     /label=polya
     misc_feature    2392..2432
                     /label=BBa_B0012
     terminator      2392..2432
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggaaaag aaattacccc gcattaaagc gctgctaacc cccggcgaag tggcgaaacg
      121 cagcggtgtg gcggtatcgg cgctgcattt ctatgaaagt aaagggttga ttaccagtat
      181 ccgtaacagc ggcaatcagc ggcgatataa acgtgatgtg ttgcgatatg ttgcaattat
      241 caaaattgct cagcgtattg gcattccgct ggcgaccatt ggtgaagcgt ttggcgtgtt
      301 gcccgaaggg catacgttaa gtgcgaaaga gtggaaacag ctttcgtccc aatggcgaga
      361 agagttggat cggcgcattc ataccttagt ggcgctgcgt gacgaactgg acggatgtat
      421 tggttgtggc tgcctttcgc gcagtgattg cccgttgcgt aacccgggcg accgcttagg
      481 agaagaaggt accggcgcac gcttgctgga agatgaacaa aactaatact agagccaggc
      541 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      601 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      661 atatactaga gaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg
      721 aattaacgaa ctgtactaga gaaagaggag aaatactaga tgcccggcga ggtgaaggcc
      781 gaccccgccg cgctcatggc ctcgctgcag ctcctgccgt cacccacgcc caacctcgag
      841 atcaagtaca ccaagatctt tattaacaat gaatggcaga actcagagag tgggagagtg
      901 ttccctgtct gtaatccagc cacaggagag caagtgtgtg aagttcaaga agcagacaag
      961 gtggatatag acaaggcagt gcaggcggcc cgcctggcct tctctcttgg ttctgtgtgg
     1021 agaaggatgg acgcttctga aagaggacgt ctgctggaca agcttgcaga cttggtggaa
     1081 cgggacaggg caactcttgc aactatggaa tcactaaatg gcggtaagcc attcctgcaa
     1141 gctttttaca tcgatttgca gggagtcatc aaaaccctga ggtattatgc aggctgggct
     1201 gataaaattc acggaatgac cattcctgta gatggagact attttacctt cacgagacat
     1261 gagcccatcg gagtgtgtgg acagattatc ccgtggaatt tccccctgct gatgttcacc
     1321 tggaaaattg ctcccgcact gtgctgtggt aacactgtgg tcatcaagcc tgcagagcag
     1381 acaccactca gtgcactgta tatgggagcc ctcatcaagg aggctggctt tccacccggg
     1441 gtcgtcaaca ttctgccagg gtatgggcca acagcagggg cagcaatagc gtctcacatt
     1501 ggcatagaca agattgcatt cacaggctct actgaggttg gaaagcttat tcaagaagca
     1561 gctggaagaa gtaatttgaa gagagtaact ctggaacttg gagggaaaag ccctaatatt
     1621 atttttgcag atgctgactt ggactacgct gtggagcagg ctcaccaggg tgtgttcttc
     1681 aaccaaggcc agtgctgcac tgcggggtct cgaatctttg tggaggagtc catctatgag
     1741 gaatttgtga aaagaagtgt ggaaagagcc aagaggcgca ttgtggggag cccctttgat
     1801 cccactactg agcagggtcc tcagattgat aagaagcaat acaacaaaat cctggagctc
     1861 atccagagcg gtgtggccga gggcgccaag ctcgagtgtg gaggcaaagg actgggaagg
     1921 aagggcttct tcattgagcc taccgtgttc tccaacgtta cagatgacat gcggatcgcc
     1981 aaggaggaga tctttggtcc tgtccaggaa attttgaggt ttaagactat ggatgaagtt
     2041 atagaaagag ccaataactc agacttcggg cttgtagcgg ctgtcttcac taatgacatc
     2101 aacaaggctc tcatggtgtc ctctgcaatg caagctggga cagtttggat caattgttac
     2161 aacgccttaa atgctcagag ccccttcggg gggttcaaga tgtctggaaa tgggagagaa
     2221 atgggtgagt ttggcttacg ggagtactca gaagtaaaga ccgtgacggt gaagatcccc
     2281 cagaagaact cctaatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2341 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2401 ctcaccttcg ggtgggcctt tctgcgttta ta
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