LOCUS       BBa_K223019             3286 bp    DNA     linear       25-Nov-2024
DEFINITION  MCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT
ACCESSION   BBa_K223019
VERSION     BBa_K223019.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..526
                     /label=BBa_K223000
     stem_loop       546..589
                     /label=stem_loop
     misc_feature    535..614
                     /label=BBa_B0010
     terminator      535..614
                     /label=BBa_B0010
     stem_loop       630..649
                     /label=T7 TE
     misc_feature    656..656
                     /label=stop
     polyA_site      650..663
                     /label=polya
     misc_feature    623..663
                     /label=BBa_B0012
     terminator      623..663
                     /label=BBa_B0012
     promoter        672..733
                     /label=BBa_K223001
     misc_feature    746..749
                     /label=conserved
     RBS             742..753
                     /label=BBa_B0034
     misc_feature    1600..1603
                     /label=conserved
     RBS             1596..1607
                     /label=BBa_B0034
     stem_loop       3169..3212
                     /label=stem_loop
     misc_feature    3158..3237
                     /label=BBa_B0010
     terminator      3158..3237
                     /label=BBa_B0010
     stem_loop       3253..3272
                     /label=T7 TE
     misc_feature    3279..3279
                     /label=stop
     polyA_site      3273..3286
                     /label=polya
     misc_feature    3246..3286
                     /label=BBa_B0012
     terminator      3246..3286
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggaaaag aaattacccc gcattaaagc gctgctaacc cccggcgaag tggcgaaacg
      121 cagcggtgtg gcggtatcgg cgctgcattt ctatgaaagt aaagggttga ttaccagtat
      181 ccgtaacagc ggcaatcagc ggcgatataa acgtgatgtg ttgcgatatg ttgcaattat
      241 caaaattgct cagcgtattg gcattccgct ggcgaccatt ggtgaagcgt ttggcgtgtt
      301 gcccgaaggg catacgttaa gtgcgaaaga gtggaaacag ctttcgtccc aatggcgaga
      361 agagttggat cggcgcattc ataccttagt ggcgctgcgt gacgaactgg acggatgtat
      421 tggttgtggc tgcctttcgc gcagtgattg cccgttgcgt aacccgggcg accgcttagg
      481 agaagaaggt accggcgcac gcttgctgga agatgaacaa aactaatact agagccaggc
      541 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      601 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      661 atatactaga gaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg
      721 aattaacgaa ctgtactaga gaaagaggag aaatactaga tgggtctgat gctgattgat
      781 tggtgtgcac tggctctggt tgttttcatt ggcctgccgc acggcgcgct ggatgctgcc
      841 atttcttttt ctatgatctc ttctgcaaaa cgcattgctc gtctggctgg tattctgctg
      901 atctatctgc tgctggcgac cgcgttcttc ctgatctggt atcagctgcc agcgtttagc
      961 ctgctgatct tcctgctgat ctccattatc cactttggta tggcagactt caacgcgtcc
     1021 ccaagcaaac tgaaatggcc gcatatcatc gcccacggcg gtgttgttac tgtttggctg
     1081 ccgctgatcc agaaaaacga agtaactaaa ctgtttagca tcctgactaa cggtccgact
     1141 ccgatcctgt gggacatcct gctgattttc ttcctgtgtt ggtctattgg cgtgtgtctg
     1201 cacacgtacg aaaccctgcg ctctaaacat tacaacatcg cctttgaact gatcggtctg
     1261 attttcctgg cgtggtatgc gccgcctctg gttacgtttg ccacttactt ctgcttcatt
     1321 cattcccgtc gccacttctc ctttgtgtgg aagcagctgc aacacatgtc ttccaaaaag
     1381 atgatgattg gcagcgcgat tatcctgtcc tgtacctctt ggctgatcgg cggtggtatc
     1441 tatttcttcc tgaactccaa aatgatcgcc tctgaggctg cgctacaaac tgtgttcatc
     1501 ggtctggcgg cactgaccgt gccgcacatg attctgatcg acttcatctt ccgtccgcac
     1561 tcttcccgta tcaaaatcaa aaactgatac tagagaaaga ggagaaatac tagatgcccg
     1621 gcgaggtgaa ggccgacccc gccgcgctca tggcctcgct gcagctcctg ccgtcaccca
     1681 cgcccaacct cgagatcaag tacaccaaga tctttattaa caatgaatgg cagaactcag
     1741 agagtgggag agtgttccct gtctgtaatc cagccacagg agagcaagtg tgtgaagttc
     1801 aagaagcaga caaggtggat atagacaagg cagtgcaggc ggcccgcctg gccttctctc
     1861 ttggttctgt gtggagaagg atggacgctt ctgaaagagg acgtctgctg gacaagcttg
     1921 cagacttggt ggaacgggac agggcaactc ttgcaactat ggaatcacta aatggcggta
     1981 agccattcct gcaagctttt tacatcgatt tgcagggagt catcaaaacc ctgaggtatt
     2041 atgcaggctg ggctgataaa attcacggaa tgaccattcc tgtagatgga gactatttta
     2101 ccttcacgag acatgagccc atcggagtgt gtggacagat tatcccgtgg aatttccccc
     2161 tgctgatgtt cacctggaaa attgctcccg cactgtgctg tggtaacact gtggtcatca
     2221 agcctgcaga gcagacacca ctcagtgcac tgtatatggg agccctcatc aaggaggctg
     2281 gctttccacc cggggtcgtc aacattctgc cagggtatgg gccaacagca ggggcagcaa
     2341 tagcgtctca cattggcata gacaagattg cattcacagg ctctactgag gttggaaagc
     2401 ttattcaaga agcagctgga agaagtaatt tgaagagagt aactctggaa cttggaggga
     2461 aaagccctaa tattattttt gcagatgctg acttggacta cgctgtggag caggctcacc
     2521 agggtgtgtt cttcaaccaa ggccagtgct gcactgcggg gtctcgaatc tttgtggagg
     2581 agtccatcta tgaggaattt gtgaaaagaa gtgtggaaag agccaagagg cgcattgtgg
     2641 ggagcccctt tgatcccact actgagcagg gtcctcagat tgataagaag caatacaaca
     2701 aaatcctgga gctcatccag agcggtgtgg ccgagggcgc caagctcgag tgtggaggca
     2761 aaggactggg aaggaagggc ttcttcattg agcctaccgt gttctccaac gttacagatg
     2821 acatgcggat cgccaaggag gagatctttg gtcctgtcca ggaaattttg aggtttaaga
     2881 ctatggatga agttatagaa agagccaata actcagactt cgggcttgta gcggctgtct
     2941 tcactaatga catcaacaag gctctcatgg tgtcctctgc aatgcaagct gggacagttt
     3001 ggatcaattg ttacaacgcc ttaaatgctc agagcccctt cggggggttc aagatgtctg
     3061 gaaatgggag agaaatgggt gagtttggct tacgggagta ctcagaagta aagaccgtga
     3121 cggtgaagat cccccagaag aactcctaat actagagcca ggcatcaaat aaaacgaaag
     3181 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     3241 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
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