BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K223020 1 BBa_K223020 WCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT 2009-07-05T11:00:00Z 2015-05-08T01:11:31Z WCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT WCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT true false _380_ 0 4533 9 Discontinued false WCP + RBS + SoxR + TT + SoxS + RBS + BLH + RBS + RALDH II + TT false Suzanne Bartram component2009908 1 BBa_K223001 component2009917 1 BBa_B0012 component2009904 1 BBa_B0012 component2009901 1 BBa_K223000 component2009898 1 BBa_J23113 component2009902 1 BBa_B0010 component2009900 1 BBa_B0034 component2009913 1 BBa_B0034 component2009915 1 BBa_B0010 component2009910 1 BBa_B0034 annotation2009910 1 BBa_B0034 range2009910 1 742 753 annotation2009913 1 BBa_B0034 range2009913 1 1596 1607 annotation2009917 1 BBa_B0012 range2009917 1 3246 3286 annotation2009901 1 BBa_K223000 range2009901 1 62 526 annotation2009915 1 BBa_B0010 range2009915 1 3158 3237 annotation2009900 1 BBa_B0034 range2009900 1 44 55 annotation2009898 1 BBa_J23113 range2009898 1 1 35 annotation2009904 1 BBa_B0012 range2009904 1 623 663 annotation2009908 1 BBa_K223001 range2009908 1 672 733 annotation2009902 1 BBa_B0010 range2009902 1 535 614 BBa_K223000 1 BBa_K223000 SoxR binds with NO to activate SoxS (NO promoter) 2009-07-04T11:00:00Z 2015-05-08T01:11:31Z paper SoxR binds with NO to activate SoxS (NO promoter) true false _380_ 0 4533 9 Discontinued false sequence false Suzanne Bartram BBa_K223001 1 BBa_K223001 SoxS promoter 2009-07-04T11:00:00Z 2015-05-08T01:11:31Z SoxS promoter Released HQ 2013 SoxS promoter true false _380_ 0 4533 9 Discontinued false SoxS promoter false Suzanne Bartram BBa_J23113 1 BBa_J23113 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K223001_sequence 1 aatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactg BBa_B0034_sequence 1 aaagaggagaaa BBa_K223000_sequence 1 atggaaaagaaattaccccgcattaaagcgctgctaacccccggcgaagtggcgaaacgcagcggtgtggcggtatcggcgctgcatttctatgaaagtaaagggttgattaccagtatccgtaacagcggcaatcagcggcgatataaacgtgatgtgttgcgatatgttgcaattatcaaaattgctcagcgtattggcattccgctggcgaccattggtgaagcgtttggcgtgttgcccgaagggcatacgttaagtgcgaaagagtggaaacagctttcgtcccaatggcgagaagagttggatcggcgcattcataccttagtggcgctgcgtgacgaactggacggatgtattggttgtggctgcctttcgcgcagtgattgcccgttgcgtaacccgggcgaccgcttaggagaagaaggtaccggcgcacgcttgctggaagatgaacaaaactaa BBa_J23113_sequence 1 ctgatggctagctcagtcctagggattatgctagc BBa_K223020_sequence 1 ctgatggctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatggaaaagaaattaccccgcattaaagcgctgctaacccccggcgaagtggcgaaacgcagcggtgtggcggtatcggcgctgcatttctatgaaagtaaagggttgattaccagtatccgtaacagcggcaatcagcggcgatataaacgtgatgtgttgcgatatgttgcaattatcaaaattgctcagcgtattggcattccgctggcgaccattggtgaagcgtttggcgtgttgcccgaagggcatacgttaagtgcgaaagagtggaaacagctttcgtcccaatggcgagaagagttggatcggcgcattcataccttagtggcgctgcgtgacgaactggacggatgtattggttgtggctgcctttcgcgcagtgattgcccgttgcgtaacccgggcgaccgcttaggagaagaaggtaccggcgcacgcttgctggaagatgaacaaaactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactgtactagagaaagaggagaaatactagatgggtctgatgctgattgattggtgtgcactggctctggttgttttcattggcctgccgcacggcgcgctggatgctgccatttctttttctatgatctcttctgcaaaacgcattgctcgtctggctggtattctgctgatctatctgctgctggcgaccgcgttcttcctgatctggtatcagctgccagcgtttagcctgctgatcttcctgctgatctccattatccactttggtatggcagacttcaacgcgtccccaagcaaactgaaatggccgcatatcatcgcccacggcggtgttgttactgtttggctgccgctgatccagaaaaacgaagtaactaaactgtttagcatcctgactaacggtccgactccgatcctgtgggacatcctgctgattttcttcctgtgttggtctattggcgtgtgtctgcacacgtacgaaaccctgcgctctaaacattacaacatcgcctttgaactgatcggtctgattttcctggcgtggtatgcgccgcctctggttacgtttgccacttacttctgcttcattcattcccgtcgccacttctcctttgtgtggaagcagctgcaacacatgtcttccaaaaagatgatgattggcagcgcgattatcctgtcctgtacctcttggctgatcggcggtggtatctatttcttcctgaactccaaaatgatcgcctctgaggctgcgctacaaactgtgttcatcggtctggcggcactgaccgtgccgcacatgattctgatcgacttcatcttccgtccgcactcttcccgtatcaaaatcaaaaactgatactagagaaagaggagaaatactagatgcccggcgaggtgaaggccgaccccgccgcgctcatggcctcgctgcagctcctgccgtcacccacgcccaacctcgagatcaagtacaccaagatctttattaacaatgaatggcagaactcagagagtgggagagtgttccctgtctgtaatccagccacaggagagcaagtgtgtgaagttcaagaagcagacaaggtggatatagacaaggcagtgcaggcggcccgcctggccttctctcttggttctgtgtggagaaggatggacgcttctgaaagaggacgtctgctggacaagcttgcagacttggtggaacgggacagggcaactcttgcaactatggaatcactaaatggcggtaagccattcctgcaagctttttacatcgatttgcagggagtcatcaaaaccctgaggtattatgcaggctgggctgataaaattcacggaatgaccattcctgtagatggagactattttaccttcacgagacatgagcccatcggagtgtgtggacagattatcccgtggaatttccccctgctgatgttcacctggaaaattgctcccgcactgtgctgtggtaacactgtggtcatcaagcctgcagagcagacaccactcagtgcactgtatatgggagccctcatcaaggaggctggctttccacccggggtcgtcaacattctgccagggtatgggccaacagcaggggcagcaatagcgtctcacattggcatagacaagattgcattcacaggctctactgaggttggaaagcttattcaagaagcagctggaagaagtaatttgaagagagtaactctggaacttggagggaaaagccctaatattatttttgcagatgctgacttggactacgctgtggagcaggctcaccagggtgtgttcttcaaccaaggccagtgctgcactgcggggtctcgaatctttgtggaggagtccatctatgaggaatttgtgaaaagaagtgtggaaagagccaagaggcgcattgtggggagcccctttgatcccactactgagcagggtcctcagattgataagaagcaatacaacaaaatcctggagctcatccagagcggtgtggccgagggcgccaagctcgagtgtggaggcaaaggactgggaaggaagggcttcttcattgagcctaccgtgttctccaacgttacagatgacatgcggatcgccaaggaggagatctttggtcctgtccaggaaattttgaggtttaagactatggatgaagttatagaaagagccaataactcagacttcgggcttgtagcggctgtcttcactaatgacatcaacaaggctctcatggtgtcctctgcaatgcaagctgggacagtttggatcaattgttacaacgccttaaatgctcagagccccttcggggggttcaagatgtctggaaatgggagagaaatgggtgagtttggcttacgggagtactcagaagtaaagaccgtgacggtgaagatcccccagaagaactcctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z