LOCUS BBa_K223021 5464 bp DNA linear 18-May-2024 DEFINITION SoxS + Part:BBa_K152005 (Promoterless Synthetic Operon of CrtE,B,I,Y and GFP) ACCESSION BBa_K223021 VERSION BBa_K223021.1 FEATURES Location/Qualifiers promoter 1..62 /label=BBa_K223001 RBS 73..80 /label=rbs CDS 88..996 /label=cds misc_feature 71..998 /label=BBa_K152001 RBS 1009..1016 /label=rbs CDS 1024..1914 /label=cds misc_feature 1007..1916 /label=BBa_K152002 misc_feature 2400..2405 /label=PstI site removed misc_feature 2811..2816 /label=PstI site removed CDS 1923..3401 /label=crtI coding sequence CDS 1923..3404 /label=BBa_K118003 RBS 3416..3422 /label=rbs CDS 3430..4578 /label=cds misc_feature 3413..4580 /label=BBa_K152003 RBS 4589..4601 /label=RBS-3\Weak RBS 4595..4598 /label=RBS misc_feature 4589..4601 /label=BBa_B0032 RBS 4589..4601 /label=BBa_B0032 CDS 4608..5327 /label=GFP protein CDS 4608..5327 /label=BBa_E0040 stem_loop 5347..5390 /label=stem_loop misc_feature 5336..5415 /label=BBa_B0010 terminator 5336..5415 /label=BBa_B0010 stem_loop 5431..5450 /label=T7 TE misc_feature 5457..5457 /label=stop polyA_site 5451..5464 /label=polya misc_feature 5424..5464 /label=BBa_B0012 terminator 5424..5464 /label=BBa_B0012 ORIGIN 1 aatcgcttta cctcaagtta acttgaggaa ttatactccc caacagatga attaacgaac 61 tgtactagag agaaggagga ttaccatatg acggtctgcg caaaaaaaca cgttcatctc 121 actcgcgatg ctgcggagca gttactggct gatattgatc gacgccttga tcagttattg 181 cccgtggagg gagaacggga tgttgtgggt gccgcgatgc gtgaaggtgc gctggcaccg 241 ggaaaacgta ttcgccccat gttgctgttg ctgaccgccc gcgatctggg ttgcgctgtc 301 agccatgacg gattactgga tttggcctgt gcggtggaaa tggtccacgc ggcttcgctg 361 atccttgacg atatgccctg catggacgat gcgaagctgc ggcgcggacg ccctaccatt 421 cattctcatt acggagagca tgtggcaata ctggcggcgg ttgccttgct gagtaaagcc 481 tttggcgtaa ttgccgatgc agatggcctc acgccgctgg caaaaaatcg ggcggtttct 541 gaactgtcaa acgccatcgg catgcaagga ttggttcagg gtcagttcaa ggatctgtct 601 gaaggggata agccgcgcag cgctgaagct attttgatga cgaatcactt taaaaccagc 661 acgctgttct gtgcctccat gcagatggcc tcgattgttg cgaatgcctc cagcgaagcg 721 cgtgattgcc tgcatcgttt ttcacttgat cttggtcagg catttcaact gctggacgat 781 ttgaccgatg gcatgaccga caccggtaag gatagcaatc aggacgccgg taaatcgacg 841 ctggtcaatc tgttaggccc gagggcggtt gaagaacgtc tgagacaaca tcttcagctt 901 gccagtgagc atctctctgc ggcctgccaa cacgggcacg ccacccaaca ttttattcag 961 gcgtggtttg acaaaaaact cgctgccgtc agttaactta ctagagagaa ggaggattac 1021 aaaatggcag ttggctcgaa aagttttgcg acagcctcaa agttatttga tgcaaaaacc 1081 cggcgcagcg tactgatgct ctacgcctgg tgccgccatt gtgacgatgt tattgacgat 1141 cagacgctgg gctttcaggc ccggcagcct gccttacaaa cgcccgaaca acgtctgatg 1201 caacttgaga tgaaaacgcg ccaggcctat gcaggatcgc agatgcacga accggcgttt 1261 gcggcttttc aggaagtggc tatggctcat gatatcgccc cggcttacgc gtttgatcat 1321 ctggaaggct tcgccatgga tgtacgcgaa gcgcaataca gccaactgga tgatacgctg 1381 cgctattgct atcacgttgc aggcgttgtc ggcttgatga tggcgcaaat catgggcgta 1441 cgggataacg ccacgctgga ccgcgcctgt gaccttgggc tggcatttca gttgaccaat 1501 attgctcgcg atattgtgga cgatgcgcat gcgggccgct gttatctgcc ggcaagctgg 1561 ctggagcatg aaggtctgaa caaagagaat tatgcggcac ctgaaaaccg tcaggcgctg 1621 agccgtatcg cccgtcgttt ggtgcaggaa gcagaacctt actatttgtc tgccacagcc 1681 ggcctggcag ggttgcccct gcgttccgcc tgggcaatcg ctacggcgaa gcaggtttac 1741 cggaaaatag gtgtcaaagt tgaacaggcc ggtcagcaag cctgggatca gcggcagtca 1801 acgaccacgc ccgaaaaatt aacgctgctg ctggccgcct ctggtcaggc ccttacttcc 1861 cggatgcggg ctcatcctcc ccgccctgcg catctctggc agcgcccgct ctaacttact 1921 agatgaaacc aactacggta attggtgcag gcttcggtgg cctggcactg gcaattcgtc 1981 tacaagctgc ggggatcccc gtcttactgc ttgaacaacg tgataaaccc ggcggtcggg 2041 cttatgtcta cgaggatcag gggtttacct ttgatgcagg cccgacggtt atcaccgatc 2101 ccagtgccat tgaagaactg tttgcactgg caggaaaaca gttaaaagag tatgtcgaac 2161 tgctgccggt tacgccgttt taccgcctgt gttgggagtc agggaaggtc tttaattacg 2221 ataacgatca aacccggctc gaagcgcaga ttcagcagtt taatccccgc gatgtcgaag 2281 gttatcgtca gtttctggac tattcacgcg cggtgtttaa agaaggctat ctaaagctcg 2341 gtactgtccc ttttttatcg ttcagagaca tgcttcgcgc cgcacctcaa ctggcgaaac 2401 tgcaagcatg gagaagcgtt tacagtaagg ttgccagtta catcgaagat gaacatctgc 2461 gccaggcgtt ttctttccac tcgctgttgg tgggcggcaa tcccttcgcc acctcatcca 2521 tttatacgtt gatacacgcg ctggagcgtg agtggggcgt ctggtttccg cgtggcggca 2581 ccggcgcatt agttcagggg atgataaagc tgtttcagga tctgggtggc gaagtcgtgt 2641 taaacgccag agtcagccat atggaaacga caggaaacaa gattgaagcc gtgcatttag 2701 aggacggtcg caggttcctg acgcaagccg tcgcgtcaaa tgcagatgtg gttcatacct 2761 atcgcgacct gttaagccag caccctgccg cggttaagca gtccaacaaa ctgcaaacta 2821 agcgcatgag taactctctg tttgtgctct attttggttt gaatcaccat catgatcagc 2881 tcgcgcatca cacggtttgt ttcggcccgc gttaccgcga gctgattgac gaaattttta 2941 atcatgatgg cctcgcagag gacttctcac tttatctgca cgcgccctgt gtcacggatt 3001 cgtcactggc gcctgaaggt tgcggcagtt actatgtgtt ggcgccggtg ccgcatttag 3061 gcaccgcgaa cctcgactgg acggttgagg ggccaaaact acgcgaccgt atttttgcgt 3121 accttgagca gcattacatg cctggcttac ggagtcagct ggtcacgcac cggatgttta 3181 cgccgtttga ttttcgcgac cagcttaatg cctatcatgg ctcagccttt tctgtggagc 3241 ccgttcttac ccagagcgcc tggtttcggc cgcataaccg cgataaaacc attactaatc 3301 tctacctggt cggcgcaggc acgcatcccg gcgcaggcat tcctggcgtc atcggctcgg 3361 caaaagcgac agcaggtttg atgctggagg atctgatata ataatactag agagaaggag 3421 gattacaaaa tgcaaccgca ttatgatctg attctcgtgg gggctggact cgcgaatggc 3481 cttatcgccc tgcgtcttca gcagcagcaa cctgatatgc gtattttgct tatcgacgcc 3541 gcaccccagg cgggcgggaa tcatacgtgg tcatttcacc acgatgattt gactgagagc 3601 caacatcgtt ggatagctcc gctggtggtt catcactggc ccgactatca ggtacgcttt 3661 cccacacgcc gtcgtaagct gaacagcggc tacttttgta ttacttctca gcgtttcgct 3721 gaggttttac agcgacagtt tggcccgcac ttgtggatgg ataccgcggt cgcagaggtt 3781 aatgcggaat ctgttcggtt gaaaaagggt caggttatcg gtgcccgcgc ggtgattgac 3841 gggcggggtt atgcggcaaa ttcagcactg agcgtgggct tccaggcgtt tattggccag 3901 gaatggcgat tgagccaccc gcatggttta tcgtctccca ttatcatgga tgccacggtc 3961 gatcagcaaa atggttatcg cttcgtgtac agcctgccgc tctcgccgac cagattgtta 4021 attgaagaca cgcactatat tgataatgcg acattagatc ctgaatgcgc gcggcaaaat 4081 atttgcgact atgccgcgca acagggttgg cagcttcaga cactgctgcg agaagaacag 4141 ggcgccttac ccattactct gtcgggcaat gccgacgcat tctggcagca gcgccccctg 4201 gcctgtagtg gattacgtgc cggtctgttc catcctacca ccggctattc actgccgctg 4261 gcggttgccg tggccgaccg cctgagtgca cttgatgtct ttacgtcggc ctcaattcac 4321 catgccatta cgcattttgc ccgcgagcgc tggcagcagc agggcttttt ccgcatgctg 4381 aatcgcatgc tgtttttagc cggacccgcc gattcacgct ggcgggttat gcagcgtttt 4441 tatggtttac ctgaagattt aattgcccgt ttttatgcgg gaaaactcac gctgaccgat 4501 cggctacgta ttctgagcgg caagccgcct gttccggtat tagcagcatt gcaagccatt 4561 atgacgactc atcgttaact tactagagtc acacaggaaa gtactagatg cgtaaaggag 4621 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc 4681 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta 4741 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg 4801 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca 4861 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga 4921 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt 4981 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact 5041 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact 5101 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa 5161 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat 5221 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa 5281 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg 5341 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 5401 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 5461 tata //