##gff-version 3
##sequence-region BBa_K223021 1 5464
BBa_K223021	.	promoter	1	62	.	+	0	ID=BBa_K223001;Name=BBa_K223001
BBa_K223021	.	CDS	1923	3404	.	+	0	ID=BBa_K118003;Name=BBa_K118003
BBa_K223021	.	point_mutation	2400	2405	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_K223021	.	point_mutation	2811	2816	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_K223021	.	CDS	1923	3401	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_K223021	.	terminator	5424	5464	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K223021	.	engineered_region	5424	5464	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K223021	.	stop_codon	5457	5457	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K223021	.	polyA_site	5451	5464	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K223021	.	stem_loop	5431	5450	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K223021	.	CDS	4608	5327	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K223021	.	CDS	4608	5327	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K223021	.	engineered_region	1007	1916	.	+	0	ID=BBa_K152002;Name=BBa_K152002
BBa_K223021	.	ribosome_entry_site	1009	1016	.	+	0	ID=annotation1999565;Name=rbs;Parent=BBa_K152002
BBa_K223021	.	CDS	1024	1914	.	+	0	ID=annotation1999566;Name=cds;Parent=BBa_K152002
BBa_K223021	.	ribosome_entry_site	4589	4601	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K223021	.	ribosome_entry_site	4589	4601	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K223021	.	engineered_region	4589	4601	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K223021	.	ribosome_entry_site	4595	4598	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K223021	.	engineered_region	3413	4580	.	+	0	ID=BBa_K152003;Name=BBa_K152003
BBa_K223021	.	CDS	3430	4578	.	+	0	ID=annotation1999564;Name=cds;Parent=BBa_K152003
BBa_K223021	.	ribosome_entry_site	3416	3422	.	+	0	ID=annotation1999563;Name=rbs;Parent=BBa_K152003
BBa_K223021	.	engineered_region	71	998	.	+	0	ID=BBa_K152001;Name=BBa_K152001
BBa_K223021	.	CDS	88	996	.	+	0	ID=annotation1999568;Name=cds;Parent=BBa_K152001
BBa_K223021	.	ribosome_entry_site	73	80	.	+	0	ID=annotation1999567;Name=rbs;Parent=BBa_K152001
BBa_K223021	.	terminator	5336	5415	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K223021	.	engineered_region	5336	5415	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K223021	.	stem_loop	5347	5390	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K223021
aatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactgtactagagagaaggagg
attaccatatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattga
tcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggca
ccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattac
tggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaa
gctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagt
aaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacg
ccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctat
tttgatgacgaatcactttaaaaccagcacgctgttctgtgcctccatgcagatggcctcgattgttgcgaatgcctcc
agcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatg
gcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggt
tgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccacccaa
cattttattcaggcgtggtttgacaaaaaactcgctgccgtcagttaacttactagagagaaggaggattacaaaatgg
cagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgc
ctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgccc
gaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcgg
cttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgt
acgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatg
gcgcaaatcatgggcgtacgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaata
ttgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaa
caaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaacct
tactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggttt
accggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaa
attaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctc
tggcagcgcccgctctaacttactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaat
tcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgag
gatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcag
gaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctt
taattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcag
tttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagaca
tgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaaga
tgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacg
ttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataa
agctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattga
agccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgc
gacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttg
tgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagct
gattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcg
tcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactgga
cggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagct
ggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggag
cccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcag
gcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgat
ataataatactagagagaaggaggattacaaaatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaa
tggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggc
gggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatc
actggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcg
tttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatct
gttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactga
gcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatgga
tgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagac
acgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggtt
ggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggca
gcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggtt
gccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcg
agcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcg
ggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcgg
ctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaactta
ctagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatta
gatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctt
tgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaact
atatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcg
agttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgt
atacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgtt
caactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcca
cacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggat
tacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttata