BBa_K223021
type: ComponentDefinition
persistent identity: BBa K223021
display id: BBa_K223021
version: 1
was generated by: iGEM to SBOL conversion
description: SoxS + Part:BBa_K152005 (Promoterless Synthetic Operon of CrtE,B,I,Y and GFP)
created: 2009-07-05T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: SoxS + Part:BBa_K152005 (Promoterless Synthetic Operon of CrtE,B,I,Y and GFP)
owned by: james
owned by: myers
mutable description: SoxS + Part:BBa_K152005 (Promoterless Synthetic Operon of CrtE,B,I,Y and GFP)
discontinued: true
dominant: false
experience: experience/None
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: SoxS + Part:BBa_K152005 (Promoterless Synthetic Operon of CrtE,B,I,Y and GFP)
star: false
creator: Suzanne Bartram
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K223001
component: BBa_K152002
component: BBa_K152001
component: BBa_K152003
component: BBa_K118003
component: BBa_E0040
component: BBa_B0032
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K223001
sequence annotation: BBa_K152001
sequence annotation: BBa_K152002
sequence annotation: BBa_K118003
sequence annotation: BBa_K152003
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K223021_sequence/1

BBa_K223001
type: Component
persistent identity: component2009921
display id: component2009921
version: 1
top level: BBa_K223021
definition: BBa_K223001
access: public

BBa_K152002
type: Component
persistent identity: component2009927
display id: component2009927
version: 1
top level: BBa_K223021
definition: BBa_K152002
access: public

BBa_K152001
type: Component
persistent identity: component2009924
display id: component2009924
version: 1
top level: BBa_K223021
definition: BBa_K152001
access: public

BBa_K152003
type: Component
persistent identity: component2009934
display id: component2009934
version: 1
top level: BBa_K223021
definition: BBa_K152003
access: public

BBa_K118003
type: Component
persistent identity: component2009931
display id: component2009931
version: 1
top level: BBa_K223021
definition: BBa_K118003
access: public

BBa_E0040
type: Component
persistent identity: component2009939
display id: component2009939
version: 1
top level: BBa_K223021
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component2009936
display id: component2009936
version: 1
top level: BBa_K223021
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component2009942
display id: component2009942
version: 1
top level: BBa_K223021
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2009940
display id: component2009940
version: 1
top level: BBa_K223021
definition: BBa_B0010
access: public

BBa_K223001
type: SequenceAnnotation
persistent identity: annotation2009921
display id: annotation2009921
version: 1
top level: BBa_K223021
location: https://synbiohub.org/public/igem/BBa_K223021/annotation2009921/range2009921/1
component: BBa_K223001

https://synbiohub.org/public/igem/BBa_K223021/annotation2009921/range2009921/1
type: Range
persistent identity: range2009921
display id: range2009921
version: 1
top level: BBa_K223021
direction: direction/reverse
start: 1
end: 62
orientation: inline

BBa_K152001
type: SequenceAnnotation
persistent identity: annotation2009924
display id: annotation2009924
version: 1
top level: BBa_K223021
location: https://synbiohub.org/public/igem/BBa_K223021/annotation2009924/range2009924/1
component: BBa_K152001

https://synbiohub.org/public/igem/BBa_K223021/annotation2009924/range2009924/1
type: Range
persistent identity: range2009924
display id: range2009924
version: 1
top level: BBa_K223021
direction: direction/reverse
start: 71
end: 998
orientation: inline

BBa_K152002
type: SequenceAnnotation
persistent identity: annotation2009927
display id: annotation2009927
version: 1
top level: BBa_K223021
location: https://synbiohub.org/public/igem/BBa_K223021/annotation2009927/range2009927/1
component: BBa_K152002

https://synbiohub.org/public/igem/BBa_K223021/annotation2009927/range2009927/1
type: Range
persistent identity: range2009927
display id: range2009927
version: 1
top level: BBa_K223021
direction: direction/reverse
start: 1007
end: 1916
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2009931
display id: annotation2009931
version: 1
top level: BBa_K223021
location: https://synbiohub.org/public/igem/BBa_K223021/annotation2009931/range2009931/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K223021/annotation2009931/range2009931/1
type: Range
persistent identity: range2009931
display id: range2009931
version: 1
top level: BBa_K223021
direction: direction/reverse
start: 1923
end: 3404
orientation: inline

BBa_K152003
type: SequenceAnnotation
persistent identity: annotation2009934
display id: annotation2009934
version: 1
top level: BBa_K223021
location: https://synbiohub.org/public/igem/BBa_K223021/annotation2009934/range2009934/1
component: BBa_K152003

https://synbiohub.org/public/igem/BBa_K223021/annotation2009934/range2009934/1
type: Range
persistent identity: range2009934
display id: range2009934
version: 1
top level: BBa_K223021
direction: direction/reverse
start: 3413
end: 4580
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2009936
display id: annotation2009936
version: 1
top level: BBa_K223021
location: https://synbiohub.org/public/igem/BBa_K223021/annotation2009936/range2009936/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K223021/annotation2009936/range2009936/1
type: Range
persistent identity: range2009936
display id: range2009936
version: 1
top level: BBa_K223021
direction: direction/forward
start: 4589
end: 4601
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2009939
display id: annotation2009939
version: 1
top level: BBa_K223021
location: https://synbiohub.org/public/igem/BBa_K223021/annotation2009939/range2009939/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K223021/annotation2009939/range2009939/1
type: Range
persistent identity: range2009939
display id: range2009939
version: 1
top level: BBa_K223021
direction: direction/reverse
start: 4608
end: 5327
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2009940
display id: annotation2009940
version: 1
top level: BBa_K223021
location: https://synbiohub.org/public/igem/BBa_K223021/annotation2009940/range2009940/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K223021/annotation2009940/range2009940/1
type: Range
persistent identity: range2009940
display id: range2009940
version: 1
top level: BBa_K223021
direction: direction/forward
start: 5336
end: 5415
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2009942
display id: annotation2009942
version: 1
top level: BBa_K223021
location: https://synbiohub.org/public/igem/BBa_K223021/annotation2009942/range2009942/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K223021/annotation2009942/range2009942/1
type: Range
persistent identity: range2009942
display id: range2009942
version: 1
top level: BBa_K223021
direction: direction/forward
start: 5424
end: 5464
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K118003
type: ComponentDefinition
persistent identity: BBa K118003
display id: BBa_K118003
version: 1
was generated by: iGEM to SBOL conversion
description: crtI coding sequence encoding phytoene dehydrogenase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtI coding sequence
sequence annotation: PstI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118003_sequence/1

crtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1995702
display id: annotation1995702
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
type: Range
persistent identity: range1995702
display id: range1995702
version: 1
top level: BBa_K118003
direction: direction/forward
start: 1
end: 1479
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968244
display id: annotation1968244
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
type: Range
persistent identity: range1968244
display id: range1968244
version: 1
top level: BBa_K118003
direction: direction/forward
start: 478
end: 483
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968245
display id: annotation1968245
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
type: Range
persistent identity: range1968245
display id: range1968245
version: 1
top level: BBa_K118003
direction: direction/forward
start: 889
end: 894
orientation: inline

BBa_K152001
type: ComponentDefinition
persistent identity: BBa K152001
display id: BBa_K152001
version: 1
was generated by: iGEM to SBOL conversion
description: rbs + crtE
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: The plasmid pAC-3.10.10.A which was sent to us by Ethan Johnson on iGEM Minnesota team timebomb.
owned by: james
owned by: myers
mutable description: crtE from Erwinia uredovora with a rbs preceeding it. This gene codes for geranylgeranyl diphosphate synthase which is the first committed step in the beta carotene synthesis pathway.
discontinued: false
dominant: false
experience: experience/None
group u list: _233_
m user id: 0
owner id: 2886
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We wanted to use this in synthetic operon construction, and as the first gene in the transcript, so we included a NdeI restriction site at the start codon.
star: false
creator: David Johnston Monje
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: rbs
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K152001_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1999567
display id: annotation1999567
version: 1
top level: BBa_K152001
location: https://synbiohub.org/public/igem/BBa_K152001/annotation1999567/range1999567/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K152001/annotation1999567/range1999567/1
type: Range
persistent identity: range1999567
display id: range1999567
version: 1
top level: BBa_K152001
direction: direction/forward
start: 3
end: 10
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1999568
display id: annotation1999568
version: 1
top level: BBa_K152001
location: https://synbiohub.org/public/igem/BBa_K152001/annotation1999568/range1999568/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K152001/annotation1999568/range1999568/1
type: Range
persistent identity: range1999568
display id: range1999568
version: 1
top level: BBa_K152001
direction: direction/forward
start: 18
end: 926
orientation: inline

BBa_K152002
type: ComponentDefinition
persistent identity: BBa K152002
display id: BBa_K152002
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + crtB
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: We got this from the plasmid pAC-3.10.10.A sent to us by Ethan Johnson of iGEM Minnesota team timebomb.
owned by: james
owned by: myers
mutable description: This is the crtB gene from Erwinia uredovora with a ribosome binding site in front. This codes for the enzyme phytoene synthase and is the second step in beta-carotene production.
discontinued: false
dominant: false
experience: experience/None
group u list: _233_
m user id: 0
owner id: 2886
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: No special considerations.
star: false
creator: David Johnston Monje
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K152002_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1999565
display id: annotation1999565
version: 1
top level: BBa_K152002
location: https://synbiohub.org/public/igem/BBa_K152002/annotation1999565/range1999565/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K152002/annotation1999565/range1999565/1
type: Range
persistent identity: range1999565
display id: range1999565
version: 1
top level: BBa_K152002
direction: direction/forward
start: 3
end: 10
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1999566
display id: annotation1999566
version: 1
top level: BBa_K152002
location: https://synbiohub.org/public/igem/BBa_K152002/annotation1999566/range1999566/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K152002/annotation1999566/range1999566/1
type: Range
persistent identity: range1999566
display id: range1999566
version: 1
top level: BBa_K152002
direction: direction/forward
start: 18
end: 908
orientation: inline

BBa_K152003
type: ComponentDefinition
persistent identity: BBa K152003
display id: BBa_K152003
version: 1
was generated by: iGEM to SBOL conversion
description: CrtY + RBS
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: The plasmid pUC-EUY which was sent to us by Ethan Johnson on iGEM Minnesota team timebomb.
owned by: james
owned by: myers
mutable description: crtE from Erwinia uredovora with a rbs preceeding it. This gene codes for lycopene cyclase which is the third step in the beta carotene synthesis pathway. In combination with CrtE and B, this works to produce lycopene, a red, antioxidant, carotenoid.
discontinued: false
dominant: false
experience: experience/None
group u list: _233_
m user id: 0
owner id: 2886
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We needed to use site directed mutagenesis to add the SpeI site after the stop codon.
star: false
creator: David Johnston Monje
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: rbs
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K152003_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1999563
display id: annotation1999563
version: 1
top level: BBa_K152003
location: https://synbiohub.org/public/igem/BBa_K152003/annotation1999563/range1999563/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K152003/annotation1999563/range1999563/1
type: Range
persistent identity: range1999563
display id: range1999563
version: 1
top level: BBa_K152003
direction: direction/forward
start: 4
end: 10
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1999564
display id: annotation1999564
version: 1
top level: BBa_K152003
location: https://synbiohub.org/public/igem/BBa_K152003/annotation1999564/range1999564/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K152003/annotation1999564/range1999564/1
type: Range
persistent identity: range1999564
display id: range1999564
version: 1
top level: BBa_K152003
direction: direction/forward
start: 18
end: 1166
orientation: inline

BBa_K223001
type: ComponentDefinition
persistent identity: BBa K223001
display id: BBa_K223001
version: 1
was generated by: iGEM to SBOL conversion
description: SoxS promoter
created: 2009-07-04T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: SoxS promoter
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: SoxS promoter
discontinued: true
dominant: false
experience: experience/None
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: SoxS promoter
star: false
creator: Suzanne Bartram
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K223001_sequence/1

https://synbiohub.org/public/igem/BBa_K223021_sequence/1
type: Sequence
persistent identity: BBa K223021 sequence
display id: BBa_K223021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactgtactagagagaaggaggattaccatatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttctgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccacccaacattttattcaggcgtggtttgacaaaaaactcgctgccgtcagttaacttactagagagaaggaggattacaaaatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtacgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaacttactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatcccc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118003_sequence/1
type: Sequence
persistent identity: BBa K118003 sequence
display id: BBa_K118003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K152001_sequence/1
type: Sequence
persistent identity: BBa K152001 sequence
display id: BBa_K152001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaaggaggattaccatatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttctgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccacccaacattttattcaggcgtggtttgacaaaaaactcgctgccgtcagttaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K152002_sequence/1
type: Sequence
persistent identity: BBa K152002 sequence
display id: BBa_K152002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaaggaggattacaaaatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtacgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K152003_sequence/1
type: Sequence
persistent identity: BBa K152003 sequence
display id: BBa_K152003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaaggaggattacaaaatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223001_sequence/1
type: Sequence
persistent identity: BBa K223001 sequence
display id: BBa_K223001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z