LOCUS       BBa_K223032             1023 bp    DNA     linear       16-Jun-2024
DEFINITION  trp-operon + RBS + GFP + TT  makes sure trp-operon functions
ACCESSION   BBa_K223032
VERSION     BBa_K223032.1  
FEATURES             Location/Qualifiers
     misc_feature    1..140
                     /label=BBa_K223023
     misc_feature    153..156
                     /label=conserved
     RBS             149..160
                     /label=BBa_B0034
     CDS             167..886
                     /label=GFP protein
     CDS             167..886
                     /label=BBa_E0040
     stem_loop       906..949
                     /label=stem_loop
     misc_feature    895..974
                     /label=BBa_B0010
     terminator      895..974
                     /label=BBa_B0010
     stem_loop       990..1009
                     /label=T7 TE
     misc_feature    1016..1016
                     /label=stop
     polyA_site      1010..1023
                     /label=polya
     misc_feature    983..1023
                     /label=BBa_B0012
     terminator      983..1023
                     /label=BBa_B0012
ORIGIN
        1 ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc
       61 aaatattctg aaatgacctg ttgacaatta atcatcgaac tagttaacta gtacgcaagt
      121 tcacgtaaaa agggtatcca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      181 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      241 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      301 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      361 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      421 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      481 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      541 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      601 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      661 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      721 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      781 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      841 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc
      901 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      961 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1021 ata
//