LOCUS       BBa_K223046             1659 bp    DNA     linear       16-Jun-2024
DEFINITION  SoxR - SoxS - GFP Reporter
ACCESSION   BBa_K223046
VERSION     BBa_K223046.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    21..526
                     /label=BBa_K223040
     stem_loop       546..589
                     /label=stem_loop
     misc_feature    535..614
                     /label=BBa_B0010
     terminator      535..614
                     /label=BBa_B0010
     stem_loop       630..649
                     /label=T7 TE
     misc_feature    656..656
                     /label=stop
     polyA_site      650..663
                     /label=polya
     misc_feature    623..663
                     /label=BBa_B0012
     terminator      623..663
                     /label=BBa_B0012
     promoter        672..776
                     /label=BBa_K223041
     misc_feature    789..792
                     /label=conserved
     RBS             785..796
                     /label=BBa_B0034
     CDS             803..1522
                     /label=GFP protein
     CDS             803..1522
                     /label=BBa_E0040
     stem_loop       1542..1585
                     /label=stem_loop
     misc_feature    1531..1610
                     /label=BBa_B0010
     terminator      1531..1610
                     /label=BBa_B0010
     stem_loop       1626..1645
                     /label=T7 TE
     misc_feature    1652..1652
                     /label=stop
     polyA_site      1646..1659
                     /label=polya
     misc_feature    1619..1659
                     /label=BBa_B0012
     terminator      1619..1659
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagag gaattcgcgg ccgcttctag atggaaaaga aattaccccg
       61 cattaaagcg ctgctaaccc ccggcgaagt ggcgaaacgc agcggtgtgg cggtatcggc
      121 gctgcatttc tatgaaagta aagggttgat taccagtatc cgtaacagcg gcaatcagcg
      181 gcgatataaa cgtgatgtgt tgcgatatgt tgcaattatc aaaattgctc agcgtattgg
      241 cattccgctg gcgaccattg gtgaagcgtt tggcgtgttg cccgaagggc atacgttaag
      301 tgcgaaagag tggaaacagc tttcgtccca atggcgagaa gagttggatc ggcgcattca
      361 taccttagtg gcgctgcgtg acgaactgga cggatgtatt ggttgtggct gcctttcgcg
      421 cagtgattgc ccgttgcgta acccgggcga ccgcttagga gaagaaggta ccggcgcacg
      481 cttgctggaa gatgaacaaa actaatacta gtagcggccg ctgcagtact agagccaggc
      541 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      601 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      661 atatactaga ggaattcgcg gccgcttcta gagaatcgct ttacctcaag ttaacttgag
      721 gaattatact ccccaacaga tgaattaacg aactgtacta gtagcggccg ctgcagtact
      781 agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      841 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      901 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      961 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
     1021 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
     1081 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
     1141 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
     1201 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     1261 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
     1321 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     1381 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     1441 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1501 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
     1561 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1621 acactggctc accttcgggt gggcctttct gcgtttata
//