LOCUS       BBa_K223047             1867 bp    DNA     linear       26-Dec-2024
DEFINITION  SoxR - SoxS - GFP Reporter with Lac Promoter
ACCESSION   BBa_K223047
VERSION     BBa_K223047.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    229..734
                     /label=BBa_K223040
     stem_loop       754..797
                     /label=stem_loop
     misc_feature    743..822
                     /label=BBa_B0010
     terminator      743..822
                     /label=BBa_B0010
     stem_loop       838..857
                     /label=T7 TE
     misc_feature    864..864
                     /label=stop
     polyA_site      858..871
                     /label=polya
     misc_feature    831..871
                     /label=BBa_B0012
     terminator      831..871
                     /label=BBa_B0012
     promoter        880..984
                     /label=BBa_K223041
     misc_feature    997..1000
                     /label=conserved
     RBS             993..1004
                     /label=BBa_B0034
     CDS             1011..1730
                     /label=GFP protein
     CDS             1011..1730
                     /label=BBa_E0040
     stem_loop       1750..1793
                     /label=stem_loop
     misc_feature    1739..1818
                     /label=BBa_B0010
     terminator      1739..1818
                     /label=BBa_B0010
     stem_loop       1834..1853
                     /label=T7 TE
     misc_feature    1860..1860
                     /label=stop
     polyA_site      1854..1867
                     /label=polya
     misc_feature    1827..1867
                     /label=BBa_B0012
     terminator      1827..1867
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagga attcgcggcc
      241 gcttctagat ggaaaagaaa ttaccccgca ttaaagcgct gctaaccccc ggcgaagtgg
      301 cgaaacgcag cggtgtggcg gtatcggcgc tgcatttcta tgaaagtaaa gggttgatta
      361 ccagtatccg taacagcggc aatcagcggc gatataaacg tgatgtgttg cgatatgttg
      421 caattatcaa aattgctcag cgtattggca ttccgctggc gaccattggt gaagcgtttg
      481 gcgtgttgcc cgaagggcat acgttaagtg cgaaagagtg gaaacagctt tcgtcccaat
      541 ggcgagaaga gttggatcgg cgcattcata ccttagtggc gctgcgtgac gaactggacg
      601 gatgtattgg ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac ccgggcgacc
      661 gcttaggaga agaaggtacc ggcgcacgct tgctggaaga tgaacaaaac taatactagt
      721 agcggccgct gcagtactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      781 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      841 tcaccttcgg gtgggccttt ctgcgtttat atactagagg aattcgcggc cgcttctaga
      901 gaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg aattaacgaa
      961 ctgtactagt agcggccgct gcagtactag agaaagagga gaaatactag atgcgtaaag
     1021 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     1081 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     1141 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     1201 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
     1261 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     1321 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     1381 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     1441 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
     1501 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     1561 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     1621 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     1681 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
     1741 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1801 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1861 gtttata
//