LOCUS       BBa_K223048             2633 bp    DNA     linear       28-Jun-2024
DEFINITION  Blh- RALDH II Coupled Generator
ACCESSION   BBa_K223048
VERSION     BBa_K223048.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    21..889
                     /label=BBa_K223042
     misc_feature    902..905
                     /label=conserved
     RBS             898..909
                     /label=BBa_B0034
     misc_feature    918..2496
                     /label=BBa_K223043
     stem_loop       2516..2559
                     /label=stem_loop
     misc_feature    2505..2584
                     /label=BBa_B0010
     terminator      2505..2584
                     /label=BBa_B0010
     stem_loop       2600..2619
                     /label=T7 TE
     misc_feature    2626..2626
                     /label=stop
     polyA_site      2620..2633
                     /label=polya
     misc_feature    2593..2633
                     /label=BBa_B0012
     terminator      2593..2633
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagag gaattcgcgg ccgcttctag atgggtctga tgctgattga
       61 ttggtgtgca ctggctctgg ttgttttcat tggcctgccg cacggcgcgc tggatgctgc
      121 catttctttt tctatgatct cttctgcaaa acgcattgct cgtctggctg gtattctgct
      181 gatctatctg ctgctggcga ccgcgttctt cctgatctgg tatcagctgc cagcgtttag
      241 cctgctgatc ttcctgctga tctccattat ccactttggt atggcagact tcaacgcgtc
      301 cccaagcaaa ctgaaatggc cgcatatcat cgcccacggc ggtgttgtta ctgtttggct
      361 gccgctgatc cagaaaaacg aagtaactaa actgtttagc atcctgacta acggtccgac
      421 tccgatcctg tgggacatcc tgctgatttt cttcctgtgt tggtctattg gcgtgtgtct
      481 gcacacgtac gaaaccctgc gctctaaaca ttacaacatc gcctttgaac tgatcggtct
      541 gattttcctg gcgtggtatg cgccgcctct ggttacgttt gccacttact tctgcttcat
      601 tcattcccgt cgccacttct cctttgtgtg gaagcagctg caacacatgt cttccaaaaa
      661 gatgatgatt ggcagcgcga ttatcctgtc ctgtacctct tggctgatcg gcggtggtat
      721 ctatttcttc ctgaactcca aaatgatcgc ctctgaggct gcgctgcaaa ctgtgttcat
      781 cggtctggcg gcactgaccg tgccgcacat gattctgatc gacttcatct tccgtccgca
      841 ctcttcccgt atcaaaatca aaaactgata ctagtagcgg ccgctgcagt actagagaaa
      901 gaggagaaat actagaggaa ttcgcggccg cttctagaga tgcccggcga ggtgaaggcc
      961 gaccccgccg cgctcatggc ctcgctgcaa ctcctgccgt cacccacgcc caacctcgag
     1021 atcaagtaca ccaagatctt tattaacaat gaatggcaga actcagagag tgggagagtg
     1081 ttccctgtct gtaatccagc cacaggagag caagtgtgtg aagttcaaga agcagacaag
     1141 gtggatatag acaaggcagt gcaggcggcc cgcctggcct tctctcttgg ttctgtgtgg
     1201 agaaggatgg acgcttctga aagaggacgt ctgctggaca agcttgcaga cttggtggaa
     1261 cgggacaggg caactcttgc aactatggaa tcactaaatg gcggtaagcc attcctgcaa
     1321 gctttttaca tcgatttgca gggagtcatc aaaaccctga ggtattatgc aggctgggct
     1381 gataaaattc acggaatgac cattcctgta gatggagact attttacctt cacgagacat
     1441 gagcccatcg gagtgtgtgg acagattatc ccgtggaatt tccccctgct gatgttcacc
     1501 tggaaaattg ctcccgcact gtgctgtggt aacactgtgg tcatcaagcc ggcagagcag
     1561 acaccactca gtgcactgta tatgggagcc ctcatcaagg aggctggctt tccacccggg
     1621 gtcgtcaaca ttctgccagg gtatgggcca acagcagggg cagcaatagc gtctcacatt
     1681 ggcatagaca agattgcatt cacaggctct actgaggttg gaaagcttat tcaagaagca
     1741 gctggaagaa gtaatttgaa gagagtaact ctggaacttg gagggaaaag ccctaatatt
     1801 atttttgcag atgctgactt ggactacgct gtggagcagg ctcaccaggg tgtgttcttc
     1861 aaccaaggcc agtgctgcac tgcggggtct cgaatctttg tggaggagtc catctatgag
     1921 gaatttgtga aaagaagtgt ggaaagagcc aagaggcgca ttgtggggag cccctttgat
     1981 cccactactg agcagggtcc tcagattgat aagaagcaat acaacaaaat cctggagctc
     2041 atccagagcg gtgtggccga gggcgccaag ctcgagtgtg gaggcaaagg actgggaagg
     2101 aagggcttct tcattgagcc taccgtgttc tccaacgtta cagatgacat gcggatcgcc
     2161 aaggaggaga tctttggtcc tgtccaggaa attttgaggt ttaagactat ggatgaagtt
     2221 atagaaagag ccaataactc agacttcggg cttgtagcgg ctgtcttcac taatgacatc
     2281 aacaaggctc tcatggtgtc ctctgcaatg caagctggga cagtttggat caattgttac
     2341 aacgccttaa atgctcagag ccccttcggg gggttcaaga tgtctggaaa tgggagagaa
     2401 atgggtgagt ttggcttacg ggagtactca gaagtaaaga ccgtgacggt gaagatcccc
     2461 cagaagaact cctaatacta gtagcggccg ctgcagtact agagccaggc atcaaataaa
     2521 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2581 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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