BBa_K223043 1 BBa_K223043 RALDH II gene: Converts retinal into retinoic acid 2009-10-16T11:00:00Z 2015-05-08T01:11:31Z RALDH II gene - altered for restriction sites http://www.jbc.org/cgi/reprint/271/27/16288 Cloning of a cDNA Encoding an Aldehyde Dehydrogenase and Its Expression in Escherichia coli ~Xianshu Wang, Peter Penzes, and Joseph L. Napoli??? The Raldh II gene converts retinal into retinoic acid. It is helpful if used in conjunction with the Blh gene for the entire process of converting beta-carotene into retinoic acid. false false _380_ 0 4533 9 Not in stock false It was altered for restriction sites. false Suzanne Bartram BBa_K223040 1 BBa_K223040 SoxR Gene: Produces the SoxR protein 2009-10-16T11:00:00Z 2015-05-08T01:11:31Z GenBank Escherichia coli E24377A http://www.ncbi.nlm.nih.gov/nuccore/NC_009801.1/from=4616553&to=4617017&report=fasta This part produces the SoxR protein, which in the presence of oxidative stresses, such as paraquat and nitric oxide, binds with high affinity to the SoxS promoter. The SoxR generator, when coupled with the SoxS promoter, can be utilized as an inducible sensor for oxidative stress. false false _380_ 0 4533 9 It's complicated false Can be put on both a high, pSB1A3 with AmpR, and low copy plasmid. false Suzanne Bartram BBa_K223041 1 BBa_K223041 SoxS Promoter 2009-10-16T11:00:00Z 2015-05-08T01:11:31Z Pseudomonas aeruginosa SoxR Does Not Conform to the Archetypal Paradigm for SoxR- Dependent Regulation of the Bacterial Oxidative Stress Adaptive Response ~Palma et al. http://iai.asm.org/cgi/reprint/73/5/2958?maxtoshow=&HITS=10&hits=10& RESULTFORMAT =&fulltext=coli&searchid=1&FIRSTINDEX=520&resourcetype=HWFIG The SoxS promoter is activated by the SoxR protein in the presence of oxidative stress, such as Paraquat or Nitric Oxide. It has a high affinity binding with the SoxR protein. It can be used in conjunction with the SoxR gene as an inducible sensor for oxidative stress. false false _380_ 0 4533 9 Not in stock false n/a false Suzanne Bartram BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K223042 1 BBa_K223042 Blh Gene: Cleaves beta-carotene into retinal 2009-10-16T11:00:00Z 2015-05-08T01:11:31Z Blh gene ??? altered for restriction sites http://www.jbc.org/cgi/reprint/M109.002618v1 In vitro characterization of a recombinant Blh protein from an uncultured marine bacterium as a &#946;-carotene 15,15&#8242;-dioxygenase ~Kim et al. **Found in Supplemental Data and used codon optimized sequence The Blh gene cleaves beta-carotene into retinal. It can be used in the pathway for the production of retinoic acid from beta-carotene in conjunction with the RALDH II genes. false false _380_ 0 4533 9 Not in stock false The optimized coding sequence was used and it was altered for restriction sites. false Suzanne Bartram BBa_K223049 1 BBa_K223049 SoxR - SoxS System with Blh and RALDH II system 2009-10-16T11:00:00Z 2015-05-08T01:11:31Z Stanford iGEM 2009 This is the superoxide inducible SoxR-SoxS promoter system coupled with the production of Blh and RALDH II genes for the cleaving of beta carotene into retinoic acid. false false _380_ 0 4533 9 Not in stock false n/a false Suzanne Bartram component2049213 1 BBa_B0010 component2049200 1 BBa_B0010 component2049202 1 BBa_B0012 component2049211 1 BBa_B0034 component2049206 1 BBa_K223041 component2049198 1 BBa_B0034 component2049199 1 BBa_K223040 component2049209 1 BBa_K223042 component2049212 1 BBa_K223043 component2049215 1 BBa_B0012 component2049208 1 BBa_B0034 annotation2049212 1 BBa_K223043 range2049212 1 1702 3280 annotation2049209 1 BBa_K223042 range2049209 1 805 1673 annotation2049202 1 BBa_B0012 range2049202 1 623 663 annotation2049213 1 BBa_B0010 range2049213 1 3289 3368 annotation2049215 1 BBa_B0012 range2049215 1 3377 3417 annotation2049198 1 BBa_B0034 range2049198 1 1 12 annotation2049199 1 BBa_K223040 range2049199 1 21 526 annotation2049206 1 BBa_K223041 range2049206 1 672 776 annotation2049200 1 BBa_B0010 range2049200 1 535 614 annotation2049211 1 BBa_B0034 range2049211 1 1682 1693 annotation2049208 1 BBa_B0034 range2049208 1 785 796 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K223043_sequence 1 gaattcgcggccgcttctagagatgcccggcgaggtgaaggccgaccccgccgcgctcatggcctcgctgcaactcctgccgtcacccacgcccaacctcgagatcaagtacaccaagatctttattaacaatgaatggcagaactcagagagtgggagagtgttccctgtctgtaatccagccacaggagagcaagtgtgtgaagttcaagaagcagacaaggtggatatagacaaggcagtgcaggcggcccgcctggccttctctcttggttctgtgtggagaaggatggacgcttctgaaagaggacgtctgctggacaagcttgcagacttggtggaacgggacagggcaactcttgcaactatggaatcactaaatggcggtaagccattcctgcaagctttttacatcgatttgcagggagtcatcaaaaccctgaggtattatgcaggctgggctgataaaattcacggaatgaccattcctgtagatggagactattttaccttcacgagacatgagcccatcggagtgtgtggacagattatcccgtggaatttccccctgctgatgttcacctggaaaattgctcccgcactgtgctgtggtaacactgtggtcatcaagccggcagagcagacaccactcagtgcactgtatatgggagccctcatcaaggaggctggctttccacccggggtcgtcaacattctgccagggtatgggccaacagcaggggcagcaatagcgtctcacattggcatagacaagattgcattcacaggctctactgaggttggaaagcttattcaagaagcagctggaagaagtaatttgaagagagtaactctggaacttggagggaaaagccctaatattatttttgcagatgctgacttggactacgctgtggagcaggctcaccagggtgtgttcttcaaccaaggccagtgctgcactgcggggtctcgaatctttgtggaggagtccatctatgaggaatttgtgaaaagaagtgtggaaagagccaagaggcgcattgtggggagcccctttgatcccactactgagcagggtcctcagattgataagaagcaatacaacaaaatcctggagctcatccagagcggtgtggccgagggcgccaagctcgagtgtggaggcaaaggactgggaaggaagggcttcttcattgagcctaccgtgttctccaacgttacagatgacatgcggatcgccaaggaggagatctttggtcctgtccaggaaattttgaggtttaagactatggatgaagttatagaaagagccaataactcagacttcgggcttgtagcggctgtcttcactaatgacatcaacaaggctctcatggtgtcctctgcaatgcaagctgggacagtttggatcaattgttacaacgccttaaatgctcagagccccttcggggggttcaagatgtctggaaatgggagagaaatgggtgagtttggcttacgggagtactcagaagtaaagaccgtgacggtgaagatcccccagaagaactcctaatactagtagcggccgctgcag BBa_B0034_sequence 1 aaagaggagaaa BBa_K223041_sequence 1 gaattcgcggccgcttctagagaatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactgtactagtagcggccgctgcag BBa_K223049_sequence 1 aaagaggagaaatactagaggaattcgcggccgcttctagatggaaaagaaattaccccgcattaaagcgctgctaacccccggcgaagtggcgaaacgcagcggtgtggcggtatcggcgctgcatttctatgaaagtaaagggttgattaccagtatccgtaacagcggcaatcagcggcgatataaacgtgatgtgttgcgatatgttgcaattatcaaaattgctcagcgtattggcattccgctggcgaccattggtgaagcgtttggcgtgttgcccgaagggcatacgttaagtgcgaaagagtggaaacagctttcgtcccaatggcgagaagagttggatcggcgcattcataccttagtggcgctgcgtgacgaactggacggatgtattggttgtggctgcctttcgcgcagtgattgcccgttgcgtaacccgggcgaccgcttaggagaagaaggtaccggcgcacgcttgctggaagatgaacaaaactaatactagtagcggccgctgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggaattcgcggccgcttctagagaatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactgtactagtagcggccgctgcagtactagagaaagaggagaaatactagaggaattcgcggccgcttctagatgggtctgatgctgattgattggtgtgcactggctctggttgttttcattggcctgccgcacggcgcgctggatgctgccatttctttttctatgatctcttctgcaaaacgcattgctcgtctggctggtattctgctgatctatctgctgctggcgaccgcgttcttcctgatctggtatcagctgccagcgtttagcctgctgatcttcctgctgatctccattatccactttggtatggcagacttcaacgcgtccccaagcaaactgaaatggccgcatatcatcgcccacggcggtgttgttactgtttggctgccgctgatccagaaaaacgaagtaactaaactgtttagcatcctgactaacggtccgactccgatcctgtgggacatcctgctgattttcttcctgtgttggtctattggcgtgtgtctgcacacgtacgaaaccctgcgctctaaacattacaacatcgcctttgaactgatcggtctgattttcctggcgtggtatgcgccgcctctggttacgtttgccacttacttctgcttcattcattcccgtcgccacttctcctttgtgtggaagcagctgcaacacatgtcttccaaaaagatgatgattggcagcgcgattatcctgtcctgtacctcttggctgatcggcggtggtatctatttcttcctgaactccaaaatgatcgcctctgaggctgcgctgcaaactgtgttcatcggtctggcggcactgaccgtgccgcacatgattctgatcgacttcatcttccgtccgcactcttcccgtatcaaaatcaaaaactgatactagtagcggccgctgcagtactagagaaagaggagaaatactagaggaattcgcggccgcttctagagatgcccggcgaggtgaaggccgaccccgccgcgctcatggcctcgctgcaactcctgccgtcacccacgcccaacctcgagatcaagtacaccaagatctttattaacaatgaatggcagaactcagagagtgggagagtgttccctgtctgtaatccagccacaggagagcaagtgtgtgaagttcaagaagcagacaaggtggatatagacaaggcagtgcaggcggcccgcctggccttctctcttggttctgtgtggagaaggatggacgcttctgaaagaggacgtctgctggacaagcttgcagacttggtggaacgggacagggcaactcttgcaactatggaatcactaaatggcggtaagccattcctgcaagctttttacatcgatttgcagggagtcatcaaaaccctgaggtattatgcaggctgggctgataaaattcacggaatgaccattcctgtagatggagactattttaccttcacgagacatgagcccatcggagtgtgtggacagattatcccgtggaatttccccctgctgatgttcacctggaaaattgctcccgcactgtgctgtggtaacactgtggtcatcaagccggcagagcagacaccactcagtgcactgtatatgggagccctcatcaaggaggctggctttccacccggggtcgtcaacattctgccagggtatgggccaacagcaggggcagcaatagcgtctcacattggcatagacaagattgcattcacaggctctactgaggttggaaagcttattcaagaagcagctggaagaagtaatttgaagagagtaactctggaacttggagggaaaagccctaatattatttttgcagatgctgacttggactacgctgtggagcaggctcaccagggtgtgttcttcaaccaaggccagtgctgcactgcggggtctcgaatctttgtggaggagtccatctatgaggaatttgtgaaaagaagtgtggaaagagccaagaggcgcattgtggggagcccctttgatcccactactgagcagggtcctcagattgataagaagcaatacaacaaaatcctggagctcatccagagcggtgtggccgagggcgccaagctcgagtgtggaggcaaaggactgggaaggaagggcttcttcattgagcctaccgtgttctccaacgttacagatgacatgcggatcgccaaggaggagatctttggtcctgtccaggaaattttgaggtttaagactatggatgaagttatagaaagagccaataactcagacttcgggcttgtagcggctgtcttcactaatgacatcaacaaggctctcatggtgtcctctgcaatgcaagctgggacagtttggatcaattgttacaacgccttaaatgctcagagccccttcggggggttcaagatgtctggaaatgggagagaaatgggtgagtttggcttacgggagtactcagaagtaaagaccgtgacggtgaagatcccccagaagaactcctaatactagtagcggccgctgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K223040_sequence 1 gaattcgcggccgcttctagatggaaaagaaattaccccgcattaaagcgctgctaacccccggcgaagtggcgaaacgcagcggtgtggcggtatcggcgctgcatttctatgaaagtaaagggttgattaccagtatccgtaacagcggcaatcagcggcgatataaacgtgatgtgttgcgatatgttgcaattatcaaaattgctcagcgtattggcattccgctggcgaccattggtgaagcgtttggcgtgttgcccgaagggcatacgttaagtgcgaaagagtggaaacagctttcgtcccaatggcgagaagagttggatcggcgcattcataccttagtggcgctgcgtgacgaactggacggatgtattggttgtggctgcctttcgcgcagtgattgcccgttgcgtaacccgggcgaccgcttaggagaagaaggtaccggcgcacgcttgctggaagatgaacaaaactaatactagtagcggccgctgcag BBa_K223042_sequence 1 gaattcgcggccgcttctagatgggtctgatgctgattgattggtgtgcactggctctggttgttttcattggcctgccgcacggcgcgctggatgctgccatttctttttctatgatctcttctgcaaaacgcattgctcgtctggctggtattctgctgatctatctgctgctggcgaccgcgttcttcctgatctggtatcagctgccagcgtttagcctgctgatcttcctgctgatctccattatccactttggtatggcagacttcaacgcgtccccaagcaaactgaaatggccgcatatcatcgcccacggcggtgttgttactgtttggctgccgctgatccagaaaaacgaagtaactaaactgtttagcatcctgactaacggtccgactccgatcctgtgggacatcctgctgattttcttcctgtgttggtctattggcgtgtgtctgcacacgtacgaaaccctgcgctctaaacattacaacatcgcctttgaactgatcggtctgattttcctggcgtggtatgcgccgcctctggttacgtttgccacttacttctgcttcattcattcccgtcgccacttctcctttgtgtggaagcagctgcaacacatgtcttccaaaaagatgatgattggcagcgcgattatcctgtcctgtacctcttggctgatcggcggtggtatctatttcttcctgaactccaaaatgatcgcctctgaggctgcgctgcaaactgtgttcatcggtctggcggcactgaccgtgccgcacatgattctgatcgacttcatcttccgtccgcactcttcccgtatcaaaatcaaaaactgatactagtagcggccgctgcag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z