BBa_K223049
type: ComponentDefinition
persistent identity: BBa K223049
display id: BBa_K223049
version: 1
was generated by: iGEM to SBOL conversion
description: SoxR - SoxS System with Blh and RALDH II system
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: Stanford iGEM 2009
owned by: james
owned by: myers
mutable description: This is the superoxide inducible SoxR-SoxS promoter system coupled with the production of Blh and RALDH II genes for the cleaving of beta carotene into retinoic acid.
discontinued: false
dominant: false
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Suzanne Bartram
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_K223040
component: BBa_K223041
component: BBa_B0012
component: BBa_K223042
component: BBa_K223043
sequence annotation: BBa_B0034
sequence annotation: BBa_K223040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K223041
sequence annotation: BBa_K223042
sequence annotation: BBa_K223043
sequence: https://synbiohub.org/public/igem/BBa_K223049_sequence/1

BBa_B0010
type: Component
persistent identity: component2049213
display id: component2049213
version: 1
top level: BBa_K223049
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component2049198
display id: component2049198
version: 1
top level: BBa_K223049
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component2049200
display id: component2049200
version: 1
top level: BBa_K223049
definition: BBa_B0010
access: public

BBa_K223040
type: Component
persistent identity: component2049199
display id: component2049199
version: 1
top level: BBa_K223049
definition: BBa_K223040
access: public

BBa_K223041
type: Component
persistent identity: component2049206
display id: component2049206
version: 1
top level: BBa_K223049
definition: BBa_K223041
access: public

BBa_B0012
type: Component
persistent identity: component2049202
display id: component2049202
version: 1
top level: BBa_K223049
definition: BBa_B0012
access: public

BBa_K223042
type: Component
persistent identity: component2049209
display id: component2049209
version: 1
top level: BBa_K223049
definition: BBa_K223042
access: public

BBa_B0034
type: Component
persistent identity: component2049208
display id: component2049208
version: 1
top level: BBa_K223049
definition: BBa_B0034
access: public

BBa_K223043
type: Component
persistent identity: component2049212
display id: component2049212
version: 1
top level: BBa_K223049
definition: BBa_K223043
access: public

BBa_B0034
type: Component
persistent identity: component2049211
display id: component2049211
version: 1
top level: BBa_K223049
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2049215
display id: component2049215
version: 1
top level: BBa_K223049
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2049198
display id: annotation2049198
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049198/range2049198/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K223049/annotation2049198/range2049198/1
type: Range
persistent identity: range2049198
display id: range2049198
version: 1
top level: BBa_K223049
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K223040
type: SequenceAnnotation
persistent identity: annotation2049199
display id: annotation2049199
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049199/range2049199/1
component: BBa_K223040

https://synbiohub.org/public/igem/BBa_K223049/annotation2049199/range2049199/1
type: Range
persistent identity: range2049199
display id: range2049199
version: 1
top level: BBa_K223049
direction: direction/reverse
start: 21
end: 526
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2049200
display id: annotation2049200
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049200/range2049200/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K223049/annotation2049200/range2049200/1
type: Range
persistent identity: range2049200
display id: range2049200
version: 1
top level: BBa_K223049
direction: direction/forward
start: 535
end: 614
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2049202
display id: annotation2049202
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049202/range2049202/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K223049/annotation2049202/range2049202/1
type: Range
persistent identity: range2049202
display id: range2049202
version: 1
top level: BBa_K223049
direction: direction/forward
start: 623
end: 663
orientation: inline

BBa_K223041
type: SequenceAnnotation
persistent identity: annotation2049206
display id: annotation2049206
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049206/range2049206/1
component: BBa_K223041

https://synbiohub.org/public/igem/BBa_K223049/annotation2049206/range2049206/1
type: Range
persistent identity: range2049206
display id: range2049206
version: 1
top level: BBa_K223049
direction: direction/reverse
start: 672
end: 776
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2049208
display id: annotation2049208
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049208/range2049208/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K223049/annotation2049208/range2049208/1
type: Range
persistent identity: range2049208
display id: range2049208
version: 1
top level: BBa_K223049
direction: direction/reverse
start: 785
end: 796
orientation: inline

BBa_K223042
type: SequenceAnnotation
persistent identity: annotation2049209
display id: annotation2049209
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049209/range2049209/1
component: BBa_K223042

https://synbiohub.org/public/igem/BBa_K223049/annotation2049209/range2049209/1
type: Range
persistent identity: range2049209
display id: range2049209
version: 1
top level: BBa_K223049
direction: direction/reverse
start: 805
end: 1673
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2049211
display id: annotation2049211
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049211/range2049211/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K223049/annotation2049211/range2049211/1
type: Range
persistent identity: range2049211
display id: range2049211
version: 1
top level: BBa_K223049
direction: direction/reverse
start: 1682
end: 1693
orientation: inline

BBa_K223043
type: SequenceAnnotation
persistent identity: annotation2049212
display id: annotation2049212
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049212/range2049212/1
component: BBa_K223043

https://synbiohub.org/public/igem/BBa_K223049/annotation2049212/range2049212/1
type: Range
persistent identity: range2049212
display id: range2049212
version: 1
top level: BBa_K223049
direction: direction/reverse
start: 1702
end: 3280
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2049213
display id: annotation2049213
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049213/range2049213/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K223049/annotation2049213/range2049213/1
type: Range
persistent identity: range2049213
display id: range2049213
version: 1
top level: BBa_K223049
direction: direction/forward
start: 3289
end: 3368
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2049215
display id: annotation2049215
version: 1
top level: BBa_K223049
location: https://synbiohub.org/public/igem/BBa_K223049/annotation2049215/range2049215/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K223049/annotation2049215/range2049215/1
type: Range
persistent identity: range2049215
display id: range2049215
version: 1
top level: BBa_K223049
direction: direction/forward
start: 3377
end: 3417
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K223040
type: ComponentDefinition
persistent identity: BBa K223040
display id: BBa_K223040
version: 1
was generated by: iGEM to SBOL conversion
description: SoxR Gene: Produces the SoxR protein
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: GenBank Escherichia coli E24377A http://www.ncbi.nlm.nih.gov/nuccore/NC_009801.1/from=4616553&to=4617017&report=fasta
owned by: james
owned by: myers
mutable description: This part produces the SoxR protein, which in the presence of oxidative stresses, such as paraquat and nitric oxide, binds with high affinity to the SoxS promoter. The SoxR generator, when coupled with the SoxS promoter, can be utilized as an inducible sensor for oxidative stress.
discontinued: false
dominant: false
experience: experience/Works
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Can be put on both a high, pSB1A3 with AmpR, and low copy plasmid.
star: false
creator: Suzanne Bartram
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K223040_sequence/1

BBa_K223041
type: ComponentDefinition
persistent identity: BBa K223041
display id: BBa_K223041
version: 1
was generated by: iGEM to SBOL conversion
description: SoxS Promoter
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: Pseudomonas aeruginosa SoxR Does Not Conform to the Archetypal Paradigm for SoxR- Dependent Regulation of the Bacterial Oxidative Stress Adaptive Response ~Palma et al. http://iai.asm.org/cgi/reprint/73/5/2958?maxtoshow=&HITS=10&hits=10& RESULTFORMAT =&fulltext=coli&searchid=1&FIRSTINDEX=520&resourcetype=HWFIG
owned by: james
owned by: myers
mutable description: The SoxS promoter is activated by the SoxR protein in the presence of oxidative stress, such as Paraquat or Nitric Oxide. It has a high affinity binding with the SoxR protein. It can be used in conjunction with the SoxR gene as an inducible sensor for oxidative stress.
discontinued: false
dominant: false
experience: experience/Works
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Suzanne Bartram
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K223041_sequence/1

BBa_K223042
type: ComponentDefinition
persistent identity: BBa K223042
display id: BBa_K223042
version: 1
was generated by: iGEM to SBOL conversion
description: Blh Gene: Cleaves beta-carotene into retinal
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: Blh gene ??? altered for restriction sites http://www.jbc.org/cgi/reprint/M109.002618v1 In vitro characterization of a recombinant Blh protein from an uncultured marine bacterium as a β-carotene 15,15′-dioxygenase ~Kim et al. **Found in Supplemental Data and used codon optimized sequence
owned by: james
owned by: myers
mutable description: The Blh gene cleaves beta-carotene into retinal. It can be used in the pathway for the production of retinoic acid from beta-carotene in conjunction with the RALDH II genes.
discontinued: false
dominant: false
experience: experience/None
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The optimized coding sequence was used and it was altered for restriction sites.
star: false
creator: Suzanne Bartram
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K223042_sequence/1

BBa_K223043
type: ComponentDefinition
persistent identity: BBa K223043
display id: BBa_K223043
version: 1
was generated by: iGEM to SBOL conversion
description: RALDH II gene: Converts retinal into retinoic acid
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: RALDH II gene - altered for restriction sites http://www.jbc.org/cgi/reprint/271/27/16288 Cloning of a cDNA Encoding an Aldehyde Dehydrogenase and Its Expression in Escherichia coli ~Xianshu Wang, Peter Penzes, and Joseph L. Napoli???
owned by: james
owned by: myers
mutable description: The Raldh II gene converts retinal into retinoic acid. It is helpful if used in conjunction with the Blh gene for the entire process of converting beta-carotene into retinoic acid.
discontinued: false
dominant: false
experience: experience/None
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It was altered for restriction sites.
star: false
creator: Suzanne Bartram
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K223043_sequence/1

https://synbiohub.org/public/igem/BBa_K223049_sequence/1
type: Sequence
persistent identity: BBa K223049 sequence
display id: BBa_K223049_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagaggaattcgcggccgcttctagatggaaaagaaattaccccgcattaaagcgctgctaacccccggcgaagtggcgaaacgcagcggtgtggcggtatcggcgctgcatttctatgaaagtaaagggttgattaccagtatccgtaacagcggcaatcagcggcgatataaacgtgatgtgttgcgatatgttgcaattatcaaaattgctcagcgtattggcattccgctggcgaccattggtgaagcgtttggcgtgttgcccgaagggcatacgttaagtgcgaaagagtggaaacagctttcgtcccaatggcgagaagagttggatcggcgcattcataccttagtggcgctgcgtgacgaactggacggatgtattggttgtggctgcctttcgcgcagtgattgcccgttgcgtaacccgggcgaccgcttaggagaagaaggtaccggcgcacgcttgctggaagatgaacaaaactaatactagtagcggccgctgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggaattcgcggccgcttctagagaatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactgtactagtagcggccgctgcagtactagagaaagaggagaaatactagaggaattcgcggccgcttctagatgggtctgatgctgattgattggtgtgcactggctctggttgttttcattggcctgccgcacggcgcgctggatgctgccatttctttttctatgatctcttctgcaaaacgcattgctcgtctggctggtattctgctgatctatctgctgctggcgaccgcgttcttcctgatctggtatcagctgccagcgtttagcctgctgatcttcctgctgatctccattatccactttggtatggcagacttcaacgcgtccccaagcaaactgaaatggccgcatatcatcgcccacggcggtgttgttactgtttggctgccgctgatccagaaaaacgaagtaactaaactgtttagcatcctgactaacggtccgactccgatcctgtgggacatcctgctgattttcttcctgtgttggtctattggcgtgtgtctgcacacgtacgaaaccctgcgctctaaacattacaacatcgcctttgaactgatcggtctgattttcctggcgtggtatgcgccgcctctggttacgtttgccacttacttctgcttcattcattcccgtcgccacttctcctttgtgtggaagcagctgcaacacatgtcttccaaaaagatgatgattggcagcgcgattatcctgtcctgtacctcttggctgatcggcggtggtatctatttcttcctgaactccaaaatgatcgcctctgaggctgcgctgcaaactgtgttcatcggtctggcggcactgaccgtgccgcacatgattctgatcgacttcatcttccgtccgcactcttcccgtatcaaaatcaaaaactgatactagtagcggccgctgcagtactagagaaagaggagaaatactagaggaattcgcggccgcttctagagatgcccggcgaggtgaaggccgaccccgccgcgctcatggcctcgctgcaactcctgccgtcacccacgcccaacctcgagatcaagtacaccaagatctttattaacaatgaatggcagaactcagagagtgggagagtgttccctgtctgtaatccagccacaggagagcaagtgtgtgaagttcaagaagcagacaaggtggatatagacaaggcagtgcaggcggcccgcctggccttctctcttggttctgtgtggagaaggatggacgctt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223040_sequence/1
type: Sequence
persistent identity: BBa K223040 sequence
display id: BBa_K223040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagatggaaaagaaattaccccgcattaaagcgctgctaacccccggcgaagtggcgaaacgcagcggtgtggcggtatcggcgctgcatttctatgaaagtaaagggttgattaccagtatccgtaacagcggcaatcagcggcgatataaacgtgatgtgttgcgatatgttgcaattatcaaaattgctcagcgtattggcattccgctggcgaccattggtgaagcgtttggcgtgttgcccgaagggcatacgttaagtgcgaaagagtggaaacagctttcgtcccaatggcgagaagagttggatcggcgcattcataccttagtggcgctgcgtgacgaactggacggatgtattggttgtggctgcctttcgcgcagtgattgcccgttgcgtaacccgggcgaccgcttaggagaagaaggtaccggcgcacgcttgctggaagatgaacaaaactaatactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223041_sequence/1
type: Sequence
persistent identity: BBa K223041 sequence
display id: BBa_K223041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagagaatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactgtactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223042_sequence/1
type: Sequence
persistent identity: BBa K223042 sequence
display id: BBa_K223042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagatgggtctgatgctgattgattggtgtgcactggctctggttgttttcattggcctgccgcacggcgcgctggatgctgccatttctttttctatgatctcttctgcaaaacgcattgctcgtctggctggtattctgctgatctatctgctgctggcgaccgcgttcttcctgatctggtatcagctgccagcgtttagcctgctgatcttcctgctgatctccattatccactttggtatggcagacttcaacgcgtccccaagcaaactgaaatggccgcatatcatcgcccacggcggtgttgttactgtttggctgccgctgatccagaaaaacgaagtaactaaactgtttagcatcctgactaacggtccgactccgatcctgtgggacatcctgctgattttcttcctgtgttggtctattggcgtgtgtctgcacacgtacgaaaccctgcgctctaaacattacaacatcgcctttgaactgatcggtctgattttcctggcgtggtatgcgccgcctctggttacgtttgccacttacttctgcttcattcattcccgtcgccacttctcctttgtgtggaagcagctgcaacacatgtcttccaaaaagatgatgattggcagcgcgattatcctgtcctgtacctcttggctgatcggcggtggtatctatttcttcctgaactccaaaatgatcgcctctgaggctgcgctgcaaactgtgttcatcggtctggcggcactgaccgtgccgcacatgattctgatcgacttcatcttccgtccgcactcttcccgtatcaaaatcaaaaactgatactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223043_sequence/1
type: Sequence
persistent identity: BBa K223043 sequence
display id: BBa_K223043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagagatgcccggcgaggtgaaggccgaccccgccgcgctcatggcctcgctgcaactcctgccgtcacccacgcccaacctcgagatcaagtacaccaagatctttattaacaatgaatggcagaactcagagagtgggagagtgttccctgtctgtaatccagccacaggagagcaagtgtgtgaagttcaagaagcagacaaggtggatatagacaaggcagtgcaggcggcccgcctggccttctctcttggttctgtgtggagaaggatggacgcttctgaaagaggacgtctgctggacaagcttgcagacttggtggaacgggacagggcaactcttgcaactatggaatcactaaatggcggtaagccattcctgcaagctttttacatcgatttgcagggagtcatcaaaaccctgaggtattatgcaggctgggctgataaaattcacggaatgaccattcctgtagatggagactattttaccttcacgagacatgagcccatcggagtgtgtggacagattatcccgtggaatttccccctgctgatgttcacctggaaaattgctcccgcactgtgctgtggtaacactgtggtcatcaagccggcagagcagacaccactcagtgcactgtatatgggagccctcatcaaggaggctggctttccacccggggtcgtcaacattctgccagggtatgggccaacagcaggggcagcaatagcgtctcacattggcatagacaagattgcattcacaggctctactgaggttggaaagcttattcaagaagcagctggaagaagtaatttgaagagagtaactctggaacttggagggaaaagccctaatattatttttgcagatgctgacttggactacgctgtggagcaggctcaccagggtgtgttcttcaaccaaggccagtgctgcactgcggggtctcgaatctttgtggaggagtccatctatgaggaatttgtgaaaagaagtgtggaaagagccaagaggcgcattgtggggagcccctttgatcccactactgagcagggtcctcagattgataagaagcaatacaacaaaatcctggagctcatccagagcggtgtggccgagggcgccaagctcgagtgtggaggcaaaggactgggaaggaagggcttcttcattgagcctaccgtgttctccaacgttacagatgacatgcggatcgccaaggaggagatctttggtcctgtccaggaaattttgaggtttaagactatggatgaagttatagaaagagccaataactcagacttcgggcttgtagcggctgtcttcactaatgacatcaacaaggctctcatggtgtcctctgcaatgcaagctgggacagtttggatcaattgttacaacgccttaaatgctcagagccccttcggggggttcaagatgtctggaaatgggagagaaatgggtgagtttggcttacgggagtactcagaagtaaagaccgtgacggtgaagatcccccagaagaactcctaatactagtagcggccgctgcag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z