##gff-version 3
##sequence-region BBa_K223050 1 3625
BBa_K223050	.	ribosome_entry_site	993	1004	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K223050	.	conserved_region	997	1000	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K223050	.	terminator	3497	3576	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K223050	.	stem_loop	3508	3551	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K223050	.	engineered_region	3497	3576	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K223050	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K223050	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K223050	.	terminator	3585	3625	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K223050	.	engineered_region	3585	3625	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K223050	.	stop_codon	3618	3618	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K223050	.	stem_loop	3592	3611	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K223050	.	polyA_site	3612	3625	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K223050	.	engineered_region	1910	3488	.	+	0	ID=BBa_K223043;Name=BBa_K223043
BBa_K223050	.	terminator	743	822	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K223050	.	stem_loop	754	797	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K223050	.	engineered_region	743	822	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K223050	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K223050	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K223050	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K223050	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K223050	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K223050	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K223050	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K223050	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K223050	.	promoter	880	984	.	+	0	ID=BBa_K223041;Name=BBa_K223041
BBa_K223050	.	engineered_region	229	734	.	+	0	ID=BBa_K223040;Name=BBa_K223040
BBa_K223050	.	terminator	831	871	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K223050	.	engineered_region	831	871	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K223050	.	stop_codon	864	864	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K223050	.	stem_loop	838	857	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K223050	.	polyA_site	858	871	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K223050	.	ribosome_entry_site	1890	1901	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K223050	.	conserved_region	1894	1897	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K223050	.	engineered_region	1013	1881	.	+	0	ID=BBa_K223042;Name=BBa_K223042
>BBa_K223050
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagaggaattcgcg
gccgcttctagatggaaaagaaattaccccgcattaaagcgctgctaacccccggcgaagtggcgaaacgcagcggtgt
ggcggtatcggcgctgcatttctatgaaagtaaagggttgattaccagtatccgtaacagcggcaatcagcggcgatat
aaacgtgatgtgttgcgatatgttgcaattatcaaaattgctcagcgtattggcattccgctggcgaccattggtgaag
cgtttggcgtgttgcccgaagggcatacgttaagtgcgaaagagtggaaacagctttcgtcccaatggcgagaagagtt
ggatcggcgcattcataccttagtggcgctgcgtgacgaactggacggatgtattggttgtggctgcctttcgcgcagt
gattgcccgttgcgtaacccgggcgaccgcttaggagaagaaggtaccggcgcacgcttgctggaagatgaacaaaact
aatactagtagcggccgctgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagaggaattcgcggccgcttctagagaatcgctttacctcaagttaacttgaggaattatactccccaacaga
tgaattaacgaactgtactagtagcggccgctgcagtactagagaaagaggagaaatactagaggaattcgcggccgct
tctagatgggtctgatgctgattgattggtgtgcactggctctggttgttttcattggcctgccgcacggcgcgctgga
tgctgccatttctttttctatgatctcttctgcaaaacgcattgctcgtctggctggtattctgctgatctatctgctg
ctggcgaccgcgttcttcctgatctggtatcagctgccagcgtttagcctgctgatcttcctgctgatctccattatcc
actttggtatggcagacttcaacgcgtccccaagcaaactgaaatggccgcatatcatcgcccacggcggtgttgttac
tgtttggctgccgctgatccagaaaaacgaagtaactaaactgtttagcatcctgactaacggtccgactccgatcctg
tgggacatcctgctgattttcttcctgtgttggtctattggcgtgtgtctgcacacgtacgaaaccctgcgctctaaac
attacaacatcgcctttgaactgatcggtctgattttcctggcgtggtatgcgccgcctctggttacgtttgccactta
cttctgcttcattcattcccgtcgccacttctcctttgtgtggaagcagctgcaacacatgtcttccaaaaagatgatg
attggcagcgcgattatcctgtcctgtacctcttggctgatcggcggtggtatctatttcttcctgaactccaaaatga
tcgcctctgaggctgcgctgcaaactgtgttcatcggtctggcggcactgaccgtgccgcacatgattctgatcgactt
catcttccgtccgcactcttcccgtatcaaaatcaaaaactgatactagtagcggccgctgcagtactagagaaagagg
agaaatactagaggaattcgcggccgcttctagagatgcccggcgaggtgaaggccgaccccgccgcgctcatggcctc
gctgcaactcctgccgtcacccacgcccaacctcgagatcaagtacaccaagatctttattaacaatgaatggcagaac
tcagagagtgggagagtgttccctgtctgtaatccagccacaggagagcaagtgtgtgaagttcaagaagcagacaagg
tggatatagacaaggcagtgcaggcggcccgcctggccttctctcttggttctgtgtggagaaggatggacgcttctga
aagaggacgtctgctggacaagcttgcagacttggtggaacgggacagggcaactcttgcaactatggaatcactaaat
ggcggtaagccattcctgcaagctttttacatcgatttgcagggagtcatcaaaaccctgaggtattatgcaggctggg
ctgataaaattcacggaatgaccattcctgtagatggagactattttaccttcacgagacatgagcccatcggagtgtg
tggacagattatcccgtggaatttccccctgctgatgttcacctggaaaattgctcccgcactgtgctgtggtaacact
gtggtcatcaagccggcagagcagacaccactcagtgcactgtatatgggagccctcatcaaggaggctggctttccac
ccggggtcgtcaacattctgccagggtatgggccaacagcaggggcagcaatagcgtctcacattggcatagacaagat
tgcattcacaggctctactgaggttggaaagcttattcaagaagcagctggaagaagtaatttgaagagagtaactctg
gaacttggagggaaaagccctaatattatttttgcagatgctgacttggactacgctgtggagcaggctcaccagggtg
tgttcttcaaccaaggccagtgctgcactgcggggtctcgaatctttgtggaggagtccatctatgaggaatttgtgaa
aagaagtgtggaaagagccaagaggcgcattgtggggagcccctttgatcccactactgagcagggtcctcagattgat
aagaagcaatacaacaaaatcctggagctcatccagagcggtgtggccgagggcgccaagctcgagtgtggaggcaaag
gactgggaaggaagggcttcttcattgagcctaccgtgttctccaacgttacagatgacatgcggatcgccaaggagga
gatctttggtcctgtccaggaaattttgaggtttaagactatggatgaagttatagaaagagccaataactcagacttc
gggcttgtagcggctgtcttcactaatgacatcaacaaggctctcatggtgtcctctgcaatgcaagctgggacagttt
ggatcaattgttacaacgccttaaatgctcagagccccttcggggggttcaagatgtctggaaatgggagagaaatggg
tgagtttggcttacgggagtactcagaagtaaagaccgtgacggtgaagatcccccagaagaactcctaatactagtag
cggccgctgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata