BBa_K223050
type: ComponentDefinition
persistent identity: BBa K223050
display id: BBa_K223050
version: 1
was generated by: iGEM to SBOL conversion
description: SoxR - SoxS System with Blh and RALDH II system and Lac promoter
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: Stanford iGEM 2009
owned by: james
owned by: myers
mutable description: Same as BBa_K223049 with the addition of an inducible lacl promoter.
discontinued: false
dominant: false
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Suzanne Bartram
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0012
component: BBa_K223042
component: BBa_B0034
component: BBa_R0010
component: BBa_B0010
component: BBa_K223040
component: BBa_K223043
component: BBa_K223041
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K223040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K223041
sequence annotation: BBa_K223042
sequence annotation: BBa_K223043
sequence: https://synbiohub.org/public/igem/BBa_K223050_sequence/1

BBa_B0012
type: Component
persistent identity: component2049231
display id: component2049231
version: 1
top level: BBa_K223050
definition: BBa_B0012
access: public

BBa_K223042
type: Component
persistent identity: component2049238
display id: component2049238
version: 1
top level: BBa_K223050
definition: BBa_K223042
access: public

BBa_B0034
type: Component
persistent identity: component2049237
display id: component2049237
version: 1
top level: BBa_K223050
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component2049227
display id: component2049227
version: 1
top level: BBa_K223050
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2049219
display id: component2049219
version: 1
top level: BBa_K223050
definition: LacI
access: public

BBa_B0010
type: Component
persistent identity: component2049229
display id: component2049229
version: 1
top level: BBa_K223050
definition: BBa_B0010
access: public

BBa_K223040
type: Component
persistent identity: component2049228
display id: component2049228
version: 1
top level: BBa_K223050
definition: BBa_K223040
access: public

BBa_K223043
type: Component
persistent identity: component2049241
display id: component2049241
version: 1
top level: BBa_K223050
definition: BBa_K223043
access: public

BBa_B0034
type: Component
persistent identity: component2049240
display id: component2049240
version: 1
top level: BBa_K223050
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2049244
display id: component2049244
version: 1
top level: BBa_K223050
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2049242
display id: component2049242
version: 1
top level: BBa_K223050
definition: BBa_B0010
access: public

BBa_K223041
type: Component
persistent identity: component2049235
display id: component2049235
version: 1
top level: BBa_K223050
definition: BBa_K223041
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2049219
display id: annotation2049219
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049219/range2049219/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K223050/annotation2049219/range2049219/1
type: Range
persistent identity: range2049219
display id: range2049219
version: 1
top level: BBa_K223050
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2049227
display id: annotation2049227
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049227/range2049227/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K223050/annotation2049227/range2049227/1
type: Range
persistent identity: range2049227
display id: range2049227
version: 1
top level: BBa_K223050
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_K223040
type: SequenceAnnotation
persistent identity: annotation2049228
display id: annotation2049228
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049228/range2049228/1
component: BBa_K223040

https://synbiohub.org/public/igem/BBa_K223050/annotation2049228/range2049228/1
type: Range
persistent identity: range2049228
display id: range2049228
version: 1
top level: BBa_K223050
direction: direction/reverse
start: 229
end: 734
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2049229
display id: annotation2049229
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049229/range2049229/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K223050/annotation2049229/range2049229/1
type: Range
persistent identity: range2049229
display id: range2049229
version: 1
top level: BBa_K223050
direction: direction/forward
start: 743
end: 822
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2049231
display id: annotation2049231
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049231/range2049231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K223050/annotation2049231/range2049231/1
type: Range
persistent identity: range2049231
display id: range2049231
version: 1
top level: BBa_K223050
direction: direction/forward
start: 831
end: 871
orientation: inline

BBa_K223041
type: SequenceAnnotation
persistent identity: annotation2049235
display id: annotation2049235
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049235/range2049235/1
component: BBa_K223041

https://synbiohub.org/public/igem/BBa_K223050/annotation2049235/range2049235/1
type: Range
persistent identity: range2049235
display id: range2049235
version: 1
top level: BBa_K223050
direction: direction/reverse
start: 880
end: 984
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2049237
display id: annotation2049237
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049237/range2049237/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K223050/annotation2049237/range2049237/1
type: Range
persistent identity: range2049237
display id: range2049237
version: 1
top level: BBa_K223050
direction: direction/reverse
start: 993
end: 1004
orientation: inline

BBa_K223042
type: SequenceAnnotation
persistent identity: annotation2049238
display id: annotation2049238
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049238/range2049238/1
component: BBa_K223042

https://synbiohub.org/public/igem/BBa_K223050/annotation2049238/range2049238/1
type: Range
persistent identity: range2049238
display id: range2049238
version: 1
top level: BBa_K223050
direction: direction/reverse
start: 1013
end: 1881
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2049240
display id: annotation2049240
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049240/range2049240/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K223050/annotation2049240/range2049240/1
type: Range
persistent identity: range2049240
display id: range2049240
version: 1
top level: BBa_K223050
direction: direction/reverse
start: 1890
end: 1901
orientation: inline

BBa_K223043
type: SequenceAnnotation
persistent identity: annotation2049241
display id: annotation2049241
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049241/range2049241/1
component: BBa_K223043

https://synbiohub.org/public/igem/BBa_K223050/annotation2049241/range2049241/1
type: Range
persistent identity: range2049241
display id: range2049241
version: 1
top level: BBa_K223050
direction: direction/reverse
start: 1910
end: 3488
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2049242
display id: annotation2049242
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049242/range2049242/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K223050/annotation2049242/range2049242/1
type: Range
persistent identity: range2049242
display id: range2049242
version: 1
top level: BBa_K223050
direction: direction/forward
start: 3497
end: 3576
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2049244
display id: annotation2049244
version: 1
top level: BBa_K223050
location: https://synbiohub.org/public/igem/BBa_K223050/annotation2049244/range2049244/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K223050/annotation2049244/range2049244/1
type: Range
persistent identity: range2049244
display id: range2049244
version: 1
top level: BBa_K223050
direction: direction/forward
start: 3585
end: 3625
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K223040
type: ComponentDefinition
persistent identity: BBa K223040
display id: BBa_K223040
version: 1
was generated by: iGEM to SBOL conversion
description: SoxR Gene: Produces the SoxR protein
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: GenBank Escherichia coli E24377A http://www.ncbi.nlm.nih.gov/nuccore/NC_009801.1/from=4616553&to=4617017&report=fasta
owned by: james
owned by: myers
mutable description: This part produces the SoxR protein, which in the presence of oxidative stresses, such as paraquat and nitric oxide, binds with high affinity to the SoxS promoter. The SoxR generator, when coupled with the SoxS promoter, can be utilized as an inducible sensor for oxidative stress.
discontinued: false
dominant: false
experience: experience/Works
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Can be put on both a high, pSB1A3 with AmpR, and low copy plasmid.
star: false
creator: Suzanne Bartram
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K223040_sequence/1

BBa_K223041
type: ComponentDefinition
persistent identity: BBa K223041
display id: BBa_K223041
version: 1
was generated by: iGEM to SBOL conversion
description: SoxS Promoter
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: Pseudomonas aeruginosa SoxR Does Not Conform to the Archetypal Paradigm for SoxR- Dependent Regulation of the Bacterial Oxidative Stress Adaptive Response ~Palma et al. http://iai.asm.org/cgi/reprint/73/5/2958?maxtoshow=&HITS=10&hits=10& RESULTFORMAT =&fulltext=coli&searchid=1&FIRSTINDEX=520&resourcetype=HWFIG
owned by: james
owned by: myers
mutable description: The SoxS promoter is activated by the SoxR protein in the presence of oxidative stress, such as Paraquat or Nitric Oxide. It has a high affinity binding with the SoxR protein. It can be used in conjunction with the SoxR gene as an inducible sensor for oxidative stress.
discontinued: false
dominant: false
experience: experience/Works
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Suzanne Bartram
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K223041_sequence/1

BBa_K223042
type: ComponentDefinition
persistent identity: BBa K223042
display id: BBa_K223042
version: 1
was generated by: iGEM to SBOL conversion
description: Blh Gene: Cleaves beta-carotene into retinal
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: Blh gene ??? altered for restriction sites http://www.jbc.org/cgi/reprint/M109.002618v1 In vitro characterization of a recombinant Blh protein from an uncultured marine bacterium as a β-carotene 15,15′-dioxygenase ~Kim et al. **Found in Supplemental Data and used codon optimized sequence
owned by: james
owned by: myers
mutable description: The Blh gene cleaves beta-carotene into retinal. It can be used in the pathway for the production of retinoic acid from beta-carotene in conjunction with the RALDH II genes.
discontinued: false
dominant: false
experience: experience/None
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The optimized coding sequence was used and it was altered for restriction sites.
star: false
creator: Suzanne Bartram
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K223042_sequence/1

BBa_K223043
type: ComponentDefinition
persistent identity: BBa K223043
display id: BBa_K223043
version: 1
was generated by: iGEM to SBOL conversion
description: RALDH II gene: Converts retinal into retinoic acid
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: RALDH II gene - altered for restriction sites http://www.jbc.org/cgi/reprint/271/27/16288 Cloning of a cDNA Encoding an Aldehyde Dehydrogenase and Its Expression in Escherichia coli ~Xianshu Wang, Peter Penzes, and Joseph L. Napoli???
owned by: james
owned by: myers
mutable description: The Raldh II gene converts retinal into retinoic acid. It is helpful if used in conjunction with the Blh gene for the entire process of converting beta-carotene into retinoic acid.
discontinued: false
dominant: false
experience: experience/None
group u list: _380_
m user id: 0
owner id: 4533
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It was altered for restriction sites.
star: false
creator: Suzanne Bartram
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K223043_sequence/1

https://synbiohub.org/public/igem/BBa_K223050_sequence/1
type: Sequence
persistent identity: BBa K223050 sequence
display id: BBa_K223050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagaggaattcgcggccgcttctagatggaaaagaaattaccccgcattaaagcgctgctaacccccggcgaagtggcgaaacgcagcggtgtggcggtatcggcgctgcatttctatgaaagtaaagggttgattaccagtatccgtaacagcggcaatcagcggcgatataaacgtgatgtgttgcgatatgttgcaattatcaaaattgctcagcgtattggcattccgctggcgaccattggtgaagcgtttggcgtgttgcccgaagggcatacgttaagtgcgaaagagtggaaacagctttcgtcccaatggcgagaagagttggatcggcgcattcataccttagtggcgctgcgtgacgaactggacggatgtattggttgtggctgcctttcgcgcagtgattgcccgttgcgtaacccgggcgaccgcttaggagaagaaggtaccggcgcacgcttgctggaagatgaacaaaactaatactagtagcggccgctgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggaattcgcggccgcttctagagaatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactgtactagtagcggccgctgcagtactagagaaagaggagaaatactagaggaattcgcggccgcttctagatgggtctgatgctgattgattggtgtgcactggctctggttgttttcattggcctgccgcacggcgcgctggatgctgccatttctttttctatgatctcttctgcaaaacgcattgctcgtctggctggtattctgctgatctatctgctgctggcgaccgcgttcttcctgatctggtatcagctgccagcgtttagcctgctgatcttcctgctgatctccattatccactttggtatggcagacttcaacgcgtccccaagcaaactgaaatggccgcatatcatcgcccacggcggtgttgttactgtttggctgccgctgatccagaaaaacgaagtaactaaactgtttagcatcctgactaacggtccgactccgatcctgtgggacatcctgctgattttcttcctgtgttggtctattggcgtgtgtctgcacacgtacgaaaccctgcgctctaaacattacaacatcgcctttgaactgatcggtctgattttcctggcgtggtatgcgccgcctctggttacgtttgccacttacttctgcttcattcattcccgtcgccacttctcctttgtgtggaagcagctgcaacacatgtcttccaaaaagatgatgattggcagcgcgattatcctgtcctgtacctcttggctgatcggcggtggtatctatttcttcctgaactccaaaatgatcgcctctgaggctgcgctgcaaactgtgttcatcggtctggcggcactgaccgtgccgcacatgattctgatcgacttcatcttccgtccgcactcttcccgtatcaaaatcaaaaactgatactagtagcggccgctgcagtactagagaaagaggagaaatactagaggaattcgcggccgcttctagagatgcccggcgaggtgaaggccgaccccgccgcgctcatggcctcgctgcaactcctgccgtcacccacg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223040_sequence/1
type: Sequence
persistent identity: BBa K223040 sequence
display id: BBa_K223040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagatggaaaagaaattaccccgcattaaagcgctgctaacccccggcgaagtggcgaaacgcagcggtgtggcggtatcggcgctgcatttctatgaaagtaaagggttgattaccagtatccgtaacagcggcaatcagcggcgatataaacgtgatgtgttgcgatatgttgcaattatcaaaattgctcagcgtattggcattccgctggcgaccattggtgaagcgtttggcgtgttgcccgaagggcatacgttaagtgcgaaagagtggaaacagctttcgtcccaatggcgagaagagttggatcggcgcattcataccttagtggcgctgcgtgacgaactggacggatgtattggttgtggctgcctttcgcgcagtgattgcccgttgcgtaacccgggcgaccgcttaggagaagaaggtaccggcgcacgcttgctggaagatgaacaaaactaatactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223041_sequence/1
type: Sequence
persistent identity: BBa K223041 sequence
display id: BBa_K223041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagagaatcgctttacctcaagttaacttgaggaattatactccccaacagatgaattaacgaactgtactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223042_sequence/1
type: Sequence
persistent identity: BBa K223042 sequence
display id: BBa_K223042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagatgggtctgatgctgattgattggtgtgcactggctctggttgttttcattggcctgccgcacggcgcgctggatgctgccatttctttttctatgatctcttctgcaaaacgcattgctcgtctggctggtattctgctgatctatctgctgctggcgaccgcgttcttcctgatctggtatcagctgccagcgtttagcctgctgatcttcctgctgatctccattatccactttggtatggcagacttcaacgcgtccccaagcaaactgaaatggccgcatatcatcgcccacggcggtgttgttactgtttggctgccgctgatccagaaaaacgaagtaactaaactgtttagcatcctgactaacggtccgactccgatcctgtgggacatcctgctgattttcttcctgtgttggtctattggcgtgtgtctgcacacgtacgaaaccctgcgctctaaacattacaacatcgcctttgaactgatcggtctgattttcctggcgtggtatgcgccgcctctggttacgtttgccacttacttctgcttcattcattcccgtcgccacttctcctttgtgtggaagcagctgcaacacatgtcttccaaaaagatgatgattggcagcgcgattatcctgtcctgtacctcttggctgatcggcggtggtatctatttcttcctgaactccaaaatgatcgcctctgaggctgcgctgcaaactgtgttcatcggtctggcggcactgaccgtgccgcacatgattctgatcgacttcatcttccgtccgcactcttcccgtatcaaaatcaaaaactgatactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223043_sequence/1
type: Sequence
persistent identity: BBa K223043 sequence
display id: BBa_K223043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagagatgcccggcgaggtgaaggccgaccccgccgcgctcatggcctcgctgcaactcctgccgtcacccacgcccaacctcgagatcaagtacaccaagatctttattaacaatgaatggcagaactcagagagtgggagagtgttccctgtctgtaatccagccacaggagagcaagtgtgtgaagttcaagaagcagacaaggtggatatagacaaggcagtgcaggcggcccgcctggccttctctcttggttctgtgtggagaaggatggacgcttctgaaagaggacgtctgctggacaagcttgcagacttggtggaacgggacagggcaactcttgcaactatggaatcactaaatggcggtaagccattcctgcaagctttttacatcgatttgcagggagtcatcaaaaccctgaggtattatgcaggctgggctgataaaattcacggaatgaccattcctgtagatggagactattttaccttcacgagacatgagcccatcggagtgtgtggacagattatcccgtggaatttccccctgctgatgttcacctggaaaattgctcccgcactgtgctgtggtaacactgtggtcatcaagccggcagagcagacaccactcagtgcactgtatatgggagccctcatcaaggaggctggctttccacccggggtcgtcaacattctgccagggtatgggccaacagcaggggcagcaatagcgtctcacattggcatagacaagattgcattcacaggctctactgaggttggaaagcttattcaagaagcagctggaagaagtaatttgaagagagtaactctggaacttggagggaaaagccctaatattatttttgcagatgctgacttggactacgctgtggagcaggctcaccagggtgtgttcttcaaccaaggccagtgctgcactgcggggtctcgaatctttgtggaggagtccatctatgaggaatttgtgaaaagaagtgtggaaagagccaagaggcgcattgtggggagcccctttgatcccactactgagcagggtcctcagattgataagaagcaatacaacaaaatcctggagctcatccagagcggtgtggccgagggcgccaagctcgagtgtggaggcaaaggactgggaaggaagggcttcttcattgagcctaccgtgttctccaacgttacagatgacatgcggatcgccaaggaggagatctttggtcctgtccaggaaattttgaggtttaagactatggatgaagttatagaaagagccaataactcagacttcgggcttgtagcggctgtcttcactaatgacatcaacaaggctctcatggtgtcctctgcaatgcaagctgggacagtttggatcaattgttacaacgccttaaatgctcagagccccttcggggggttcaagatgtctggaaatgggagagaaatgggtgagtttggcttacgggagtactcagaagtaaagaccgtgacggtgaagatcccccagaagaactcctaatactagtagcggccgctgcag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z