LOCUS       BBa_K223059             1585 bp    DNA     linear       18-Feb-2025
DEFINITION  mutTrp (5MT) Inverter with GFP Biobrick part
ACCESSION   BBa_K223059
VERSION     BBa_K223059.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    21..388
                     /label=BBa_K223052
     stem_loop       408..451
                     /label=stem_loop
     misc_feature    397..476
                     /label=BBa_B0010
     terminator      397..476
                     /label=BBa_B0010
     stem_loop       492..511
                     /label=T7 TE
     misc_feature    518..518
                     /label=stop
     polyA_site      512..525
                     /label=polya
     misc_feature    485..525
                     /label=BBa_B0012
     terminator      485..525
                     /label=BBa_B0012
     promoter        534..702
                     /label=BBa_K223051
     misc_feature    715..718
                     /label=conserved
     RBS             711..722
                     /label=BBa_B0034
     CDS             729..1448
                     /label=GFP protein
     CDS             729..1448
                     /label=BBa_E0040
     stem_loop       1468..1511
                     /label=stem_loop
     misc_feature    1457..1536
                     /label=BBa_B0010
     terminator      1457..1536
                     /label=BBa_B0010
     stem_loop       1552..1571
                     /label=T7 TE
     misc_feature    1578..1578
                     /label=stop
     polyA_site      1572..1585
                     /label=polya
     misc_feature    1545..1585
                     /label=BBa_B0012
     terminator      1545..1585
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagag gaattcgcgg ccgcttctag atggcccaac aatcacccta
       61 ttcagcagcg atggcagaac agcgtcacca ggagtggtta cgttttgtcg acctgcttaa
      121 gaatgcctac caaaacgatc tccatttacc gttgttaaac ctgatgctga cgccagatga
      181 gcgcgaagcg ttggggactc gcgtgcgtat tctggaagag ctgttgcgcg gcgaaatgag
      241 ccagcgtgag ttaaaaaatg aactcggcgc aggcaaagcg acgattacgc gtggatctaa
      301 cagcctgaaa gccgcgcccg tcgagctgcg ccagtggctg gaagaggtgt tgctgaaaag
      361 cgattgatac tagtagcggc cgctgcagta ctagagccag gcatcaaata aaacgaaagg
      421 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      481 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggaattcg
      541 cggccgcttc tagagctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat
      601 cataacggtt ctggcaaata ttctgaaatg acctgttgac aattaatcat cgacctagtt
      661 acctaggacg caaggtcacg ttactagtag cggccgctgc agtactagag aaagaggaga
      721 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      781 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      841 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      901 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
      961 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1021 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     1081 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1141 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     1201 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     1261 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1321 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1381 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1441 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1501 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1561 tcgggtgggc ctttctgcgt ttata
//