LOCUS       BBa_K223060             1793 bp    DNA     linear       04-Jul-2024
DEFINITION  5MT Inverter-GFP Reporter and Lac Promoter
ACCESSION   BBa_K223060
VERSION     BBa_K223060.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    229..596
                     /label=BBa_K223052
     stem_loop       616..659
                     /label=stem_loop
     misc_feature    605..684
                     /label=BBa_B0010
     terminator      605..684
                     /label=BBa_B0010
     stem_loop       700..719
                     /label=T7 TE
     misc_feature    726..726
                     /label=stop
     polyA_site      720..733
                     /label=polya
     misc_feature    693..733
                     /label=BBa_B0012
     terminator      693..733
                     /label=BBa_B0012
     promoter        742..910
                     /label=BBa_K223051
     misc_feature    923..926
                     /label=conserved
     RBS             919..930
                     /label=BBa_B0034
     CDS             937..1656
                     /label=GFP protein
     CDS             937..1656
                     /label=BBa_E0040
     stem_loop       1676..1719
                     /label=stem_loop
     misc_feature    1665..1744
                     /label=BBa_B0010
     terminator      1665..1744
                     /label=BBa_B0010
     stem_loop       1760..1779
                     /label=T7 TE
     misc_feature    1786..1786
                     /label=stop
     polyA_site      1780..1793
                     /label=polya
     misc_feature    1753..1793
                     /label=BBa_B0012
     terminator      1753..1793
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagga attcgcggcc
      241 gcttctagat ggcccaacaa tcaccctatt cagcagcgat ggcagaacag cgtcaccagg
      301 agtggttacg ttttgtcgac ctgcttaaga atgcctacca aaacgatctc catttaccgt
      361 tgttaaacct gatgctgacg ccagatgagc gcgaagcgtt ggggactcgc gtgcgtattc
      421 tggaagagct gttgcgcggc gaaatgagcc agcgtgagtt aaaaaatgaa ctcggcgcag
      481 gcaaagcgac gattacgcgt ggatctaaca gcctgaaagc cgcgcccgtc gagctgcgcc
      541 agtggctgga agaggtgttg ctgaaaagcg attgatacta gtagcggccg ctgcagtact
      601 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      661 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      721 ttctgcgttt atatactaga ggaattcgcg gccgcttcta gagctcaagg cgcactcccg
      781 ttctggataa tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgac
      841 ctgttgacaa ttaatcatcg acctagttac ctaggacgca aggtcacgtt actagtagcg
      901 gccgctgcag tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
      961 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1021 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1081 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1141 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1201 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1261 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1321 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1381 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1441 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1501 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1561 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1621 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
     1681 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1741 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//