##gff-version 3
##sequence-region BBa_K223060 1 1793
BBa_K223060	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K223060	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K223060	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K223060	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K223060	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K223060	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K223060	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K223060	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K223060	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K223060	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K223060	.	promoter	742	910	.	+	0	ID=BBa_K223051;Name=BBa_K223051
BBa_K223060	.	terminator	1753	1793	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K223060	.	stop_codon	1786	1786	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K223060	.	polyA_site	1780	1793	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K223060	.	stem_loop	1760	1779	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K223060	.	engineered_region	1753	1793	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K223060	.	CDS	937	1656	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K223060	.	CDS	937	1656	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K223060	.	engineered_region	229	596	.	+	0	ID=BBa_K223052;Name=BBa_K223052
BBa_K223060	.	terminator	1665	1744	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K223060	.	engineered_region	1665	1744	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K223060	.	stem_loop	1676	1719	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K223060	.	terminator	693	733	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K223060	.	stop_codon	726	726	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K223060	.	polyA_site	720	733	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K223060	.	stem_loop	700	719	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K223060	.	engineered_region	693	733	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K223060	.	ribosome_entry_site	919	930	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K223060	.	conserved_region	923	926	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K223060	.	terminator	605	684	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K223060	.	engineered_region	605	684	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K223060	.	stem_loop	616	659	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K223060
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagaggaattcgcg
gccgcttctagatggcccaacaatcaccctattcagcagcgatggcagaacagcgtcaccaggagtggttacgttttgt
cgacctgcttaagaatgcctaccaaaacgatctccatttaccgttgttaaacctgatgctgacgccagatgagcgcgaa
gcgttggggactcgcgtgcgtattctggaagagctgttgcgcggcgaaatgagccagcgtgagttaaaaaatgaactcg
gcgcaggcaaagcgacgattacgcgtggatctaacagcctgaaagccgcgcccgtcgagctgcgccagtggctggaaga
ggtgttgctgaaaagcgattgatactagtagcggccgctgcagtactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagaggaattcgcggccgcttctagagctcaaggcgcactcccgttctggataa
tgttttttgcgccgacatcataacggttctggcaaatattctgaaatgacctgttgacaattaatcatcgacctagtta
cctaggacgcaaggtcacgttactagtagcggccgctgcagtactagagaaagaggagaaatactagatgcgtaaagga
gaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtg
gagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatg
gccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgacttt
ttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtg
ctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacat
tcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaa
gttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattg
gcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagag
agaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata