LOCUS BBa_K223062 8498 bp DNA linear 25-Nov-2024 DEFINITION TolC and Hemolysin B, C and D gene complex ACCESSION BBa_K223062 VERSION BBa_K223062.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 21..1543 /label=BBa_K223055 misc_feature 1556..1559 /label=conserved RBS 1552..1563 /label=BBa_B0034 misc_feature 1572..4640 /label=BBa_K223056 misc_feature 4653..4656 /label=conserved RBS 4649..4660 /label=BBa_B0034 misc_feature 4669..8361 /label=BBa_K223057 stem_loop 8381..8424 /label=stem_loop misc_feature 8370..8449 /label=BBa_B0010 terminator 8370..8449 /label=BBa_B0010 stem_loop 8465..8484 /label=T7 TE misc_feature 8491..8491 /label=stop polyA_site 8485..8498 /label=polya misc_feature 8458..8498 /label=BBa_B0012 terminator 8458..8498 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagag gaattcgcgg ccgcttctag atgaagaaat tgctccccat 61 tcttatcggc ctgagccttt ctgggttcag ttcgttgagc caggccgaga acctgatgca 121 agtttatcag caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg ccgatcgtga 181 tgctgccttt gaaaaaatta atgaagcgcg cagtccatta ctgccacagc taggtttagg 241 tgcagattac acctatagca acggctaccg cgacgcgaac ggcatcaact ctaacgcgac 301 cagtgcgtcc ttgcagttaa ctcaatccat ttttgatatg tcgaaatggc gtgcgttaac 361 gctgcaggaa aaagcagcag ggattcagga cgtcacgtat cagaccgatc agcaaacctt 421 gatcctcaac accgcgaccg cttatttcaa cgtgttgaat gctattgacg ttctttccta 481 tacacaggca caaaaagaag cgatctaccg tcaattagat caaaccaccc aacgttttaa 541 cgtgggcctg gtagcgatca ccgacgtgca gaacgcccgc gcacagtacg ataccgtgct 601 ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg gtagagcagc tgcgccagat 661 caccggtaac tactatccgg aactggctgc gctgaatgtc gaaaacttta aaaccgacaa 721 accacagccg gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt cgctgttaca 781 ggcacgcttg agccaggacc tggcgcgcga gcaaattcgc caggcgcagg atggtcactt 841 accgactctg gatttaacgg cttctaccgg gatttctgac acctcttata gcggttcgaa 901 aacccgtggt gccgctggta cccagtatga cgatagcaat atgggccaga acaaagttgg 961 cctgagcttc tcgctgccga tttatcaggg cggaatggtt aactcgcagg tgaaacaggc 1021 acagtacaac tttgtcggtg ccagcgagca actggaaagt gcccatcgta gcgtcgtgca 1081 gaccgtgcgt tcctccttca acaacattaa tgcatctatc agtagcatta acgcctacaa 1141 acaagccgta gtttccgctc aaagctcatt agacgcgatg gaagcgggct actcggtcgg 1201 tacgcgtacc attgttgatg tgttggatgc gaccaccacg ttgtacaacg ccaagcaaga 1261 gctggcgaat gcgcgttata actacctgat taatcagctg aatattaagt cagctctggg 1321 tacgttgaac gagcaggatc tgctggcact gaacaatgcg ctgagcaaac cggtttccac 1381 taatccggaa aacgttgcac cgcaaacgcc ggaacagaat gctattgctg atggttatgc 1441 gcctgatagc ccggcaccag tcgttcagca aacatccgca cgcactacca ccagtaacgg 1501 tcataaccct ttccgtaact gatactagta gcggccgctg cagtactaga gaaagaggag 1561 aaatactaga ggaattcgcg gccgcttcta gaggaattcc aagcgaagtc catccccctc 1621 cctcttgatt acaagggtga taattattat tcgcatttgt gtggtaatgg gatagaaagg 1681 aatggataga aaaagaacaa aattagtata gcaatagata tgcccactgc attgaatact 1741 tacagggcat tattttatta tgtttaaatt gaagtggtct ctggtttgat ttatttgtta 1801 ttcaaggggg ctgtttggag atcggaaaat tctgtacgtt aagtgtatta tttaaccagt 1861 ttcgatgcgt aacagattga ttttgcgtca gcggttatcg cttttaagtt gttgctcttg 1921 cgctatcgcg tttaggttat ccgattaaag tcaaatttcc tgaaaatgct gtatagcgcg 1981 ggagtgcacc ttatagctgt aggtaagtat gttcaaaaaa tagtcttgcc gtacaataat 2041 tttccatatc caaactcact ccttcaagat tctggtcccg gtttacgggt agtttccgga 2101 agggcggtag catgctgatt caaactgcaa gatgaaacat tgtcggagtt ggatggaatt 2161 aagtcatggc tatagcattt gggcgtgcat aacaaaattg gtcctcatat tttagagtat 2221 gattgcatat tcactaatat ttttactttc tgatgcgtgg tggcatcatg ctttatgaga 2281 taaacaatcc tggtagacta gccccctgaa tctccagaca accaatatca cttatttaag 2341 tgatagtctt aatactagtt tttagactag tcattggaga acagatgatt gatgtcttag 2401 gatcggagaa acgcagacgg cgtactacac aggaaaagat cgctatcgtt cagcagagct 2461 ttgaaccggg aatgacggtc tcccttgttg cccggcaaca tggtgtggca gccagccagc 2521 tatttctctg gcgcaagcaa taccaggagg gaagtcttac tgctgtggct gccggagaac 2581 aggtcgttcc tgcctctgaa cttgctgccg ccatgaagca gattaaagag ctccagcgcc 2641 tgctcggcaa aaaaacgatg gaaaatgaac tccttaaaga agccgttgaa tatgggcgag 2701 caaaaaagtg gatagcgcac gcgcccttat tgcccgggga tggggagtaa gcttcgtcag 2761 ccgttgtctc cgggtgtcgc gtgcgcagtt gcacgtcatt ctcagacgaa ccgatgactg 2821 gaaggacggc cgccgcagcc gtcacacgga tgatacggat gtgcttcgcc gtatacatca 2881 tgttatcgga gagctgccca catatggtta tcgtcgggta tgggcgctgc ttcgcagaca 2941 aacagaactt gatggtatgc ctgcgatcaa tgccaaatgt gtttaccgga tcatgtgcca 3001 gaatgcgctg ttgcttgagc gaaaacccgc tgtaccgcca tcgaaacggg cacataccgg 3061 cagagtggct gtgaaagaaa gtaatcagcg atggtgctct gacgggtttg agttccgctg 3121 tgataacgga gaaaaactgc gggtcacgtt cgcgctggac tgctgtgatc gtgaggcact 3181 gcactgggcg gtcacaacgg gtggcttcaa cagtgaaaca gtacaggacg tcatgctggg 3241 agcagtggaa cgccgctttg gcagcgagct tccggcgtct ccagtggagt ggctgacgga 3301 taatggttca tgctaccggg cgaatgaaac acgtcagttc gccaggatgt tgggacttga 3361 accgaagaac acggcagtgc ggagtccgga gagtaacgga ataacagaga gcttcgtgaa 3421 aacgataaag cgtgattaca taagtatcat gcccaaacca gacgggttaa cggcagcaaa 3481 gaaccttgca gaggcgttcg agcattataa cgaatggcat ccgcatagtg cgctgggtta 3541 tcgctcgcca cgggaatatc tgcggcagcg ggccagtaat gggttaagtg ataacaggta 3601 tctggaaata taggggcaaa tccacctggt cattatctgg aatttgacga agtgtgataa 3661 ctggtatagc cagattaatc taaacctttg tctgacaaaa tcagataaag aagagtagtt 3721 caaaagacaa ctcgtggact ctcattcaga gagataggcg ttaccaaaat ttgtttggaa 3781 ctgaacaaga aaattgtatt tgtgtaacta taatcttaat gtaaaataaa agacaccagt 3841 tctgtagaat atgcttattg aagagagtgt aataataatt ttatatagat gttgtacaaa 3901 gaacaggaat gagtaattat ttatgcttga tgttttttga ctcttgcttt ttatagttat 3961 tatttttaag ttagtcagcg caataaaaac ttgcttttaa tattaatgcg agttatgaca 4021 ttaaacggaa gaaacataaa ggcatatttt tgccacaata tttaatcata taatttaagt 4081 tgtagtgagt ttattatgaa tataaacaaa ccattagaga ttcttgggca tgtatcctgg 4141 ctatgggcca gttctccact acacagaaac tggccagtat ctttgtttgc aataaatgta 4201 ttacccgcaa tacaggctaa ccaatatgtt ttattaaccc gggatgatta ccctgtcgcg 4261 tattgtagtt gggctaattt aagtttagaa aatgaaatta aatatcttaa tgatgttacc 4321 tcattagttg cagaagactg gacttcaggt gatcgtaaat ggttcattga ctggattgct 4381 cctttcgggg ataacggtgc cctgtacaaa tatatgcgaa aaaaattccc tgatgaacta 4441 ttcagagcca tcagggtgga tcccaaaact catgttggta aagtatcaga atttcatgga 4501 ggtaaaattg ataaacagtt agcgaataaa atttttaaac aatatcacca cgagttaata 4561 actgaagtaa aaagaaagtc agattttaat ttttcattaa ctggttaaga ggtaattaat 4621 actagtagcg gccgctgcag tactagagaa agaggagaaa tactagagga attcgcggcc 4681 gcttctagag tatattaatt taaatgatag caatcttact gggctgtgcc acataagatt 4741 gctatttttt ttggagtcat aatggattct tgtcataaaa ttgattatgg gttatacgcc 4801 ctggagattt tagcccaata ccataacgtc tctgttaacc cggaagaaat taaacataga 4861 tttgatacag acgggacagg tctgggatta acgtcatggt tgcttgctgc gaaatcttta 4921 gaactaaagg taaaacaggt aaaaaaaaca attgatcgat taaactttat ttttctgccc 4981 gcattagtct ggagagagga tggacgtcat tttattctga ctaaaatcag caaagaagta 5041 aacagatatc ttatttttga tttggagcag cgaaatcccc gtgttctcga acagtctgag 5101 tttgaggcgt tatatcaggg gcatattatt cttattactt cccgttcttc tgttaccggg 5161 aaactggcaa aatttgactt tacctggttt attcctgcca ttataaaata caggagaata 5221 tttattgaaa cccttgttgt atctgttttt ttacaattat ttgcattaat aacccccctt 5281 tttttccagg tggttatgga caaagtatta gtgcacaggg ggttttcaac ccttaatgtt 5341 attactgttg cattatctgt tgtagtggtg tttgagatta tactcagcgg tttaagaact 5401 tacatttttg cacatagtac aagtcggatt gatgttgagt tgggtgccaa actcttccgg 5461 catttactgg cgctaccgat ctcttatttt gagagtcgtc gtgttggtga tactgttgcg 5521 agggtaagag aattagacca gatccgtaat tttctgacag gacaggcatt aacatctgtt 5581 ttggacttat tattttcact catatttttt gcggtaatgt ggtattacag cccaaagctt 5641 actctggtga tcttattttc gctgccttgt tatgctgcat ggtctgtttt tattagcccc 5701 attttgcgac gtcgccttga tgataagttt tcacggaatg cggataatca atctttcctg 5761 gtggaatcag taacggcgat taacactata aaagctatgg cagtctcacc tcagatgacg 5821 aacatatggg acaaacaatt ggcaggatat gttgctgcag gctttaaagt gacagtatta 5881 gcaaccattg gtcaacaagg aatacagtta atacaaaaga ctgttatgat catcaaccta 5941 tggttgggag cacacctggt tatttccggg gatttaagta ttggtcagtt aattgctttt 6001 aatatgcttg ctggtcagat tgttgcaccg gttattcgcc ttgcacaaat ctggcaggat 6061 ttccagcagg ttggtatatc agttacccgc cttggtgatg tgcttaactc tccaactgaa 6121 agttatcatg ggaaactgac attgccggaa attaatggtg atatcacttt tcgtaatatc 6181 cggtttcgct ataaacctga ttctccggtt attttggaca atatcaatct tagtattaag 6241 cagggggagg ttattggtat tgtcggacgt tctggttcag gaaaaagcac attaactaaa 6301 ttaattcaac gtttttatat tcctgaaaat ggccaggtat taattgatgg acatgatctt 6361 gcgttggctg atcctaactg gttacgtcgt caggtggggg ttgtgttgca ggacaatgtg 6421 ctgcttaatc gcagtattat tgataatatt tcactggcta atcctggcat gtccgtcgaa 6481 aaagttattt atgcagcgaa attagcaggc gctcatgatt ttatttctga tttgcgtgag 6541 gggtataaca ccattgtcgg ggaacagggg gcaggattat ccggaggtca acgtcaacgc 6601 atcgcaattg caagggcgct ggtgaacaac cctaaaatac tcatttttga tgaagcaacc 6661 agtgctctgg attatgagtc ggagcatgtc atcatgcgca atatgcacaa aatatgtaag 6721 ggcagaacgg ttataatcat tgctcatcgt ctgtctacag taaaaaatgc agaccgcatt 6781 attgtcatgg aaaaagggaa aattgttgaa cagggtaaac ataaggagct gctttctgaa 6841 ccggaaagtt tatacagtta cttatatcag ttacagtcag actaacagaa agaacagaag 6901 aatatgaaaa catggttaat ggggttcagc gagttcctgt tgcgctataa acttgtctgg 6961 agtgaaacat ggaaaatccg gaagcaatta gatactccgg tacgtgaaaa ggacgaaaat 7021 gagttcttac ccgctcatct ggaattaatt gaaacgccag tatccagacg gccgcgtctg 7081 gttgcttatt ttattatggg gtttctggtt attgctttta ttttatctgt tttaggccaa 7141 gtggaaattg ttgccactgc aaatgggaaa ttaacacaca gtgggcgtag taaagaaatt 7201 aaacctattg aaaactcaat agttaaagaa attatcgtaa aagaaggaga gtcagtccgg 7261 aaaggggatg tgttattaaa gcttacagca ctgggagctg aagctgatac gttaaaaaca 7321 cagtcatcac tgttacaggc caggctggaa caaactcggt atcaaattct gagcaggtca 7381 attgaattaa ataaactacc tgaactaaag cttcctgatg agccttattt tcagaatgta 7441 tctgaagagg aagtactgcg tttaacttct ttgataaaag aacagttttc cacatggcaa 7501 aatcagaagt atcaaaaaga actgaatttg gataagaaaa gagcagagcg attaacagta 7561 cttgcccgta taaaccgtta tgaaaattta tcaagggttg aaaaaagccg tctggatgat 7621 ttcagtagtt tattgcataa acaggcaatt gcaaaacatg ctgtacttga gcaggagaat 7681 aaatatgtcg aagcagtaaa tgaattacga gtttataaat cacaactgga gcaaattgag 7741 agtgagatat tgtctgcaaa agaagaatat cagcttgtta cgcagctttt taaaaatgaa 7801 attttagata agctaagaca aacaacagac aacattgggt tattaactct ggaattagcg 7861 aaaaatgaag agcgtcaaca ggcttcagta atcagggccc cagtttcggg aaaagttcag 7921 caactgaagg ttcatactga aggtggggtt gttacaacag cggaaacact gatggtcatc 7981 gttccggaag atgacacgct ggaggttact gctctggtac aaaataaaga tattggtttt 8041 attaacgtcg ggcagaatgc catcattaaa gtggaggcat ttccttatac acgatatggt 8101 tatctggtgg gtaaggtgaa aaatataaat ttagatgcaa tagaagacca gagactggga 8161 cttgttttta atgttattat ttctattgaa gagaattgtt tgtcaaccgg gaataaaaac 8221 attccattaa gctcgggtat ggcagtcact gcagaaataa agacaggtat gcgaagtgta 8281 atcagttatc ttcttagtcc tttagaagag tcagtaacag aaagtttacg tgagcgttaa 8341 tactagtagc ggccgctgca gtactagagc caggcatcaa ataaaacgaa aggctcagtc 8401 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 8461 cactggctca ccttcgggtg ggcctttctg cgtttata //