BBa_K223057 1 BBa_K223057 Rear Flanking Sequence (HlyB and HlyD) 2009-10-17T11:00:00Z 2015-05-08T01:11:31Z Compiled from several different sources: 1. GenBank E. coli str. CFT073 http://www.ncbi.nlm.nih.gov/nuccore/NC_004431.1/from=3422041&to=3424164&report=fasta 2. GenBank E. coli str. CFT073 http://www.ncbi.nlm.nih.gov/nuccore/NC_004431.1/from=3424183&to=3425619&report=fasta Along with parts BBa_K223055 and K223056, this part helps form the channel protein for the extracellular transport of HlyAs-fused protein. false false _380_ 0 4529 9 Not in stock false Stanford iGEM Team 2009 altered sequences for internal restriction sites false Anusuya Ramasubramanian BBa_K223056 1 BBa_K223056 Front Flanking Sequence (HlyR, IS1, HlyC) 2009-10-17T11:00:00Z 2015-05-08T01:11:31Z Compiled from several different papers: 1. Cloning of a cDNA Encoding an Aldehyde Dehydrogenase and Its Expression in Escherichia coli Xianshu Wang, Peter Penzes, and Joseph L. Napoli THE JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 271, No. 27, Issue of July 5, pp. 16288 ???16293, 1996 2. Escherichia coli CFT073 (GenBank) http://www.ncbi.nlm.nih.gov/nuccore/NC_004431.1/from=3418210&to=3418884&report=fasta This front flanking sequence has three of the hemolysin parts - HlyR, Is1 and HlyC. In conjunction with rear flanking sequence, containing HlyB and HlyD, and TolC (part K223055), this part creates a channel protein to facilitate the extracellular export HlyAs-fused proteins. false false _380_ 0 4529 9 Not in stock false n/a false Anusuya Ramasubramanian BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K223062 1 BBa_K223062 TolC and Hemolysin B, C and D gene complex 2009-10-17T11:00:00Z 2015-05-08T01:11:31Z Synthesized by Stanford iGEM 2009 This a composite part of BBa_K223055, BBa_K223056, BBa_K223057 that provides all necessary genes to create a channel protein in the extra cellular membrane of a prokaryotic cell. This channel can be used to export large organic molecules fused to a signal sequence, HlyAs false false _380_ 0 4529 9 Not in stock false n/a false Anusuya Ramasubramanian component2051703 1 BBa_B0034 component2051704 1 BBa_K223055 component2051713 1 BBa_B0012 component2051710 1 BBa_K223057 component2051707 1 BBa_K223056 component2051706 1 BBa_B0034 component2051711 1 BBa_B0010 component2051709 1 BBa_B0034 annotation2051706 1 BBa_B0034 range2051706 1 1552 1563 annotation2051707 1 BBa_K223056 range2051707 1 1572 4640 annotation2051704 1 BBa_K223055 range2051704 1 21 1543 annotation2051713 1 BBa_B0012 range2051713 1 8458 8498 annotation2051709 1 BBa_B0034 range2051709 1 4649 4660 annotation2051710 1 BBa_K223057 range2051710 1 4669 8361 annotation2051711 1 BBa_B0010 range2051711 1 8370 8449 annotation2051703 1 BBa_B0034 range2051703 1 1 12 BBa_K223055 1 BBa_K223055 TolC gene 2009-10-17T11:00:00Z 2015-05-08T01:11:31Z GenBank Escherichia coli str. K-12 substr. MG1655 http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.2/from=3176137&to=3177618&report=fasta Stanford iGEM Team 2009 altered the sequence restriction sites and unknown portions of the sequence. TolC, along with HemolysinB, C, and D, for a channel in the cellular membrane to allow for the extracellular transport of HlyAs-fused protein sequences. The TolC receptor, in conjunction to the Hly genes, can serve as channel for transporting large proteins. false false _380_ 0 4529 9 Not in stock false n/a false Anusuya Ramasubramanian BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K223057_sequence 1 gaattcgcggccgcttctagagtatattaatttaaatgatagcaatcttactgggctgtgccacataagattgctattttttttggagtcataatggattcttgtcataaaattgattatgggttatacgccctggagattttagcccaataccataacgtctctgttaacccggaagaaattaaacatagatttgatacagacgggacaggtctgggattaacgtcatggttgcttgctgcgaaatctttagaactaaaggtaaaacaggtaaaaaaaacaattgatcgattaaactttatttttctgcccgcattagtctggagagaggatggacgtcattttattctgactaaaatcagcaaagaagtaaacagatatcttatttttgatttggagcagcgaaatccccgtgttctcgaacagtctgagtttgaggcgttatatcaggggcatattattcttattacttcccgttcttctgttaccgggaaactggcaaaatttgactttacctggtttattcctgccattataaaatacaggagaatatttattgaaacccttgttgtatctgtttttttacaattatttgcattaataacccccctttttttccaggtggttatggacaaagtattagtgcacagggggttttcaacccttaatgttattactgttgcattatctgttgtagtggtgtttgagattatactcagcggtttaagaacttacatttttgcacatagtacaagtcggattgatgttgagttgggtgccaaactcttccggcatttactggcgctaccgatctcttattttgagagtcgtcgtgttggtgatactgttgcgagggtaagagaattagaccagatccgtaattttctgacaggacaggcattaacatctgttttggacttattattttcactcatattttttgcggtaatgtggtattacagcccaaagcttactctggtgatcttattttcgctgccttgttatgctgcatggtctgtttttattagccccattttgcgacgtcgccttgatgataagttttcacggaatgcggataatcaatctttcctggtggaatcagtaacggcgattaacactataaaagctatggcagtctcacctcagatgacgaacatatgggacaaacaattggcaggatatgttgctgcaggctttaaagtgacagtattagcaaccattggtcaacaaggaatacagttaatacaaaagactgttatgatcatcaacctatggttgggagcacacctggttatttccggggatttaagtattggtcagttaattgcttttaatatgcttgctggtcagattgttgcaccggttattcgccttgcacaaatctggcaggatttccagcaggttggtatatcagttacccgccttggtgatgtgcttaactctccaactgaaagttatcatgggaaactgacattgccggaaattaatggtgatatcacttttcgtaatatccggtttcgctataaacctgattctccggttattttggacaatatcaatcttagtattaagcagggggaggttattggtattgtcggacgttctggttcaggaaaaagcacattaactaaattaattcaacgtttttatattcctgaaaatggccaggtattaattgatggacatgatcttgcgttggctgatcctaactggttacgtcgtcaggtgggggttgtgttgcaggacaatgtgctgcttaatcgcagtattattgataatatttcactggctaatcctggcatgtccgtcgaaaaagttatttatgcagcgaaattagcaggcgctcatgattttatttctgatttgcgtgaggggtataacaccattgtcggggaacagggggcaggattatccggaggtcaacgtcaacgcatcgcaattgcaagggcgctggtgaacaaccctaaaatactcatttttgatgaagcaaccagtgctctggattatgagtcggagcatgtcatcatgcgcaatatgcacaaaatatgtaagggcagaacggttataatcattgctcatcgtctgtctacagtaaaaaatgcagaccgcattattgtcatggaaaaagggaaaattgttgaacagggtaaacataaggagctgctttctgaaccggaaagtttatacagttacttatatcagttacagtcagactaacagaaagaacagaagaatatgaaaacatggttaatggggttcagcgagttcctgttgcgctataaacttgtctggagtgaaacatggaaaatccggaagcaattagatactccggtacgtgaaaaggacgaaaatgagttcttacccgctcatctggaattaattgaaacgccagtatccagacggccgcgtctggttgcttattttattatggggtttctggttattgcttttattttatctgttttaggccaagtggaaattgttgccactgcaaatgggaaattaacacacagtgggcgtagtaaagaaattaaacctattgaaaactcaatagttaaagaaattatcgtaaaagaaggagagtcagtccggaaaggggatgtgttattaaagcttacagcactgggagctgaagctgatacgttaaaaacacagtcatcactgttacaggccaggctggaacaaactcggtatcaaattctgagcaggtcaattgaattaaataaactacctgaactaaagcttcctgatgagccttattttcagaatgtatctgaagaggaagtactgcgtttaacttctttgataaaagaacagttttccacatggcaaaatcagaagtatcaaaaagaactgaatttggataagaaaagagcagagcgattaacagtacttgcccgtataaaccgttatgaaaatttatcaagggttgaaaaaagccgtctggatgatttcagtagtttattgcataaacaggcaattgcaaaacatgctgtacttgagcaggagaataaatatgtcgaagcagtaaatgaattacgagtttataaatcacaactggagcaaattgagagtgagatattgtctgcaaaagaagaatatcagcttgttacgcagctttttaaaaatgaaattttagataagctaagacaaacaacagacaacattgggttattaactctggaattagcgaaaaatgaagagcgtcaacaggcttcagtaatcagggccccagtttcgggaaaagttcagcaactgaaggttcatactgaaggtggggttgttacaacagcggaaacactgatggtcatcgttccggaagatgacacgctggaggttactgctctggtacaaaataaagatattggttttattaacgtcgggcagaatgccatcattaaagtggaggcatttccttatacacgatatggttatctggtgggtaaggtgaaaaatataaatttagatgcaatagaagaccagagactgggacttgtttttaatgttattatttctattgaagagaattgtttgtcaaccgggaataaaaacattccattaagctcgggtatggcagtcactgcagaaataaagacaggtatgcgaagtgtaatcagttatcttcttagtcctttagaagagtcagtaacagaaagtttacgtgagcgttaatactagtagcggccgctgcag BBa_K223056_sequence 1 gaattcgcggccgcttctagaggaattccaagcgaagtccatccccctccctcttgattacaagggtgataattattattcgcatttgtgtggtaatgggatagaaaggaatggatagaaaaagaacaaaattagtatagcaatagatatgcccactgcattgaatacttacagggcattattttattatgtttaaattgaagtggtctctggtttgatttatttgttattcaagggggctgtttggagatcggaaaattctgtacgttaagtgtattatttaaccagtttcgatgcgtaacagattgattttgcgtcagcggttatcgcttttaagttgttgctcttgcgctatcgcgtttaggttatccgattaaagtcaaatttcctgaaaatgctgtatagcgcgggagtgcaccttatagctgtaggtaagtatgttcaaaaaatagtcttgccgtacaataattttccatatccaaactcactccttcaagattctggtcccggtttacgggtagtttccggaagggcggtagcatgctgattcaaactgcaagatgaaacattgtcggagttggatggaattaagtcatggctatagcatttgggcgtgcataacaaaattggtcctcatattttagagtatgattgcatattcactaatatttttactttctgatgcgtggtggcatcatgctttatgagataaacaatcctggtagactagccccctgaatctccagacaaccaatatcacttatttaagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttaggatcggagaaacgcagacggcgtactacacaggaaaagatcgctatcgttcagcagagctttgaaccgggaatgacggtctcccttgttgcccggcaacatggtgtggcagccagccagctatttctctggcgcaagcaataccaggagggaagtcttactgctgtggctgccggagaacaggtcgttcctgcctctgaacttgctgccgccatgaagcagattaaagagctccagcgcctgctcggcaaaaaaacgatggaaaatgaactccttaaagaagccgttgaatatgggcgagcaaaaaagtggatagcgcacgcgcccttattgcccggggatggggagtaagcttcgtcagccgttgtctccgggtgtcgcgtgcgcagttgcacgtcattctcagacgaaccgatgactggaaggacggccgccgcagccgtcacacggatgatacggatgtgcttcgccgtatacatcatgttatcggagagctgcccacatatggttatcgtcgggtatgggcgctgcttcgcagacaaacagaacttgatggtatgcctgcgatcaatgccaaatgtgtttaccggatcatgtgccagaatgcgctgttgcttgagcgaaaacccgctgtaccgccatcgaaacgggcacataccggcagagtggctgtgaaagaaagtaatcagcgatggtgctctgacgggtttgagttccgctgtgataacggagaaaaactgcgggtcacgttcgcgctggactgctgtgatcgtgaggcactgcactgggcggtcacaacgggtggcttcaacagtgaaacagtacaggacgtcatgctgggagcagtggaacgccgctttggcagcgagcttccggcgtctccagtggagtggctgacggataatggttcatgctaccgggcgaatgaaacacgtcagttcgccaggatgttgggacttgaaccgaagaacacggcagtgcggagtccggagagtaacggaataacagagagcttcgtgaaaacgataaagcgtgattacataagtatcatgcccaaaccagacgggttaacggcagcaaagaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggccagtaatgggttaagtgataacaggtatctggaaatataggggcaaatccacctggtcattatctggaatttgacgaagtgtgataactggtatagccagattaatctaaacctttgtctgacaaaatcagataaagaagagtagttcaaaagacaactcgtggactctcattcagagagataggcgttaccaaaatttgtttggaactgaacaagaaaattgtatttgtgtaactataatcttaatgtaaaataaaagacaccagttctgtagaatatgcttattgaagagagtgtaataataattttatatagatgttgtacaaagaacaggaatgagtaattatttatgcttgatgttttttgactcttgctttttatagttattatttttaagttagtcagcgcaataaaaacttgcttttaatattaatgcgagttatgacattaaacggaagaaacataaaggcatatttttgccacaatatttaatcatataatttaagttgtagtgagtttattatgaatataaacaaaccattagagattcttgggcatgtatcctggctatgggccagttctccactacacagaaactggccagtatctttgtttgcaataaatgtattacccgcaatacaggctaaccaatatgttttattaacccgggatgattaccctgtcgcgtattgtagttgggctaatttaagtttagaaaatgaaattaaatatcttaatgatgttacctcattagttgcagaagactggacttcaggtgatcgtaaatggttcattgactggattgctcctttcggggataacggtgccctgtacaaatatatgcgaaaaaaattccctgatgaactattcagagccatcagggtggatcccaaaactcatgttggtaaagtatcagaatttcatggaggtaaaattgataaacagttagcgaataaaatttttaaacaatatcaccacgagttaataactgaagtaaaaagaaagtcagattttaatttttcattaactggttaagaggtaattaatactagtagcggccgctgcag BBa_B0034_sequence 1 aaagaggagaaa BBa_K223062_sequence 1 aaagaggagaaatactagaggaattcgcggccgcttctagatgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggccgagaacctgatgcaagtttatcagcaagcacgccttagtaacccggaattgcgtaagtctgccgccgatcgtgatgctgcctttgaaaaaattaatgaagcgcgcagtccattactgccacagctaggtttaggtgcagattacacctatagcaacggctaccgcgacgcgaacggcatcaactctaacgcgaccagtgcgtccttgcagttaactcaatccatttttgatatgtcgaaatggcgtgcgttaacgctgcaggaaaaagcagcagggattcaggacgtcacgtatcagaccgatcagcaaaccttgatcctcaacaccgcgaccgcttatttcaacgtgttgaatgctattgacgttctttcctatacacaggcacaaaaagaagcgatctaccgtcaattagatcaaaccacccaacgttttaacgtgggcctggtagcgatcaccgacgtgcagaacgcccgcgcacagtacgataccgtgctggcgaacgaagtgaccgcacgtaataaccttgataacgcggtagagcagctgcgccagatcaccggtaactactatccggaactggctgcgctgaatgtcgaaaactttaaaaccgacaaaccacagccggttaacgcgctgctgaaagaagccgaaaaacgcaacctgtcgctgttacaggcacgcttgagccaggacctggcgcgcgagcaaattcgccaggcgcaggatggtcacttaccgactctggatttaacggcttctaccgggatttctgacacctcttatagcggttcgaaaacccgtggtgccgctggtacccagtatgacgatagcaatatgggccagaacaaagttggcctgagcttctcgctgccgatttatcagggcggaatggttaactcgcaggtgaaacaggcacagtacaactttgtcggtgccagcgagcaactggaaagtgcccatcgtagcgtcgtgcagaccgtgcgttcctccttcaacaacattaatgcatctatcagtagcattaacgcctacaaacaagccgtagtttccgctcaaagctcattagacgcgatggaagcgggctactcggtcggtacgcgtaccattgttgatgtgttggatgcgaccaccacgttgtacaacgccaagcaagagctggcgaatgcgcgttataactacctgattaatcagctgaatattaagtcagctctgggtacgttgaacgagcaggatctgctggcactgaacaatgcgctgagcaaaccggtttccactaatccggaaaacgttgcaccgcaaacgccggaacagaatgctattgctgatggttatgcgcctgatagcccggcaccagtcgttcagcaaacatccgcacgcactaccaccagtaacggtcataaccctttccgtaactgatactagtagcggccgctgcagtactagagaaagaggagaaatactagaggaattcgcggccgcttctagaggaattccaagcgaagtccatccccctccctcttgattacaagggtgataattattattcgcatttgtgtggtaatgggatagaaaggaatggatagaaaaagaacaaaattagtatagcaatagatatgcccactgcattgaatacttacagggcattattttattatgtttaaattgaagtggtctctggtttgatttatttgttattcaagggggctgtttggagatcggaaaattctgtacgttaagtgtattatttaaccagtttcgatgcgtaacagattgattttgcgtcagcggttatcgcttttaagttgttgctcttgcgctatcgcgtttaggttatccgattaaagtcaaatttcctgaaaatgctgtatagcgcgggagtgcaccttatagctgtaggtaagtatgttcaaaaaatagtcttgccgtacaataattttccatatccaaactcactccttcaagattctggtcccggtttacgggtagtttccggaagggcggtagcatgctgattcaaactgcaagatgaaacattgtcggagttggatggaattaagtcatggctatagcatttgggcgtgcataacaaaattggtcctcatattttagagtatgattgcatattcactaatatttttactttctgatgcgtggtggcatcatgctttatgagataaacaatcctggtagactagccccctgaatctccagacaaccaatatcacttatttaagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttaggatcggagaaacgcagacggcgtactacacaggaaaagatcgctatcgttcagcagagctttgaaccgggaatgacggtctcccttgttgcccggcaacatggtgtggcagccagccagctatttctctggcgcaagcaataccaggagggaagtcttactgctgtggctgccggagaacaggtcgttcctgcctctgaacttgctgccgccatgaagcagattaaagagctccagcgcctgctcggcaaaaaaacgatggaaaatgaactccttaaagaagccgttgaatatgggcgagcaaaaaagtggatagcgcacgcgcccttattgcccggggatggggagtaagcttcgtcagccgttgtctccgggtgtcgcgtgcgcagttgcacgtcattctcagacgaaccgatgactggaaggacggccgccgcagccgtcacacggatgatacggatgtgcttcgccgtatacatcatgttatcggagagctgcccacatatggttatcgtcgggtatgggcgctgcttcgcagacaaacagaacttgatggtatgcctgcgatcaatgccaaatgtgtttaccggatcatgtgccagaatgcgctgttgcttgagcgaaaacccgctgtaccgccatcgaaacgggcacataccggcagagtggctgtgaaagaaagtaatcagcgatggtgctctgacgggtttgagttccgctgtgataacggagaaaaactgcgggtcacgttcgcgctggactgctgtgatcgtgaggcactgcactgggcggtcacaacgggtggcttcaacagtgaaacagtacaggacgtcatgctgggagcagtggaacgccgctttggcagcgagcttccggcgtctccagtggagtggctgacggataatggttcatgctaccgggcgaatgaaacacgtcagttcgccaggatgttgggacttgaaccgaagaacacggcagtgcggagtccggagagtaacggaataacagagagcttcgtgaaaacgataaagcgtgattacataagtatcatgcccaaaccagacgggttaacggcagcaaagaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggccagtaatgggttaagtgataacaggtatctggaaatataggggcaaatccacctggtcattatctggaatttgacgaagtgtgataactggtatagccagattaatctaaacctttgtctgacaaaatcagataaagaagagtagttcaaaagacaactcgtggactctcattcagagagataggcgttaccaaaatttgtttggaactgaacaagaaaattgtatttgtgtaactataatcttaatgtaaaataaaagacaccagttctgtagaatatgcttattgaagagagtgtaataataattttatatagatgttgtacaaagaacaggaatgagtaattatttatgcttgatgttttttgactcttgctttttatagttattatttttaagttagtcagcgcaataaaaacttgcttttaatattaatgcgagttatgacattaaacggaagaaacataaaggcatatttttgccacaatatttaatcatataatttaagttgtagtgagtttattatgaatataaacaaaccattagagattcttgggcatgtatcctggctatgggccagttctccactacacagaaactggccagtatctttgtttgcaataaatgtattacccgcaatacaggctaaccaatatgttttattaacccgggatgattaccctgtcgcgtattgtagttgggctaatttaagtttagaaaatgaaattaaatatcttaatgatgttacctcattagttgcagaagactggacttcaggtgatcgtaaatggttcattgactggattgctcctttcggggataacggtgccctgtacaaatatatgcgaaaaaaattccctgatgaactattcagagccatcagggtggatcccaaaactcatgttggtaaagtatcagaatttcatggaggtaaaattgataaacagttagcgaataaaatttttaaacaatatcaccacgagttaataactgaagtaaaaagaaagtcagattttaatttttcattaactggttaagaggtaattaatactagtagcggccgctgcagtactagagaaagaggagaaatactagaggaattcgcggccgcttctagagtatattaatttaaatgatagcaatcttactgggctgtgccacataagattgctattttttttggagtcataatggattcttgtcataaaattgattatgggttatacgccctggagattttagcccaataccataacgtctctgttaacccggaagaaattaaacatagatttgatacagacgggacaggtctgggattaacgtcatggttgcttgctgcgaaatctttagaactaaaggtaaaacaggtaaaaaaaacaattgatcgattaaactttatttttctgcccgcattagtctggagagaggatggacgtcattttattctgactaaaatcagcaaagaagtaaacagatatcttatttttgatttggagcagcgaaatccccgtgttctcgaacagtctgagtttgaggcgttatatcaggggcatattattcttattacttcccgttcttctgttaccgggaaactggcaaaatttgactttacctggtttattcctgccattataaaatacaggagaatatttattgaaacccttgttgtatctgtttttttacaattatttgcattaataacccccctttttttccaggtggttatggacaaagtattagtgcacagggggttttcaacccttaatgttattactgttgcattatctgttgtagtggtgtttgagattatactcagcggtttaagaacttacatttttgcacatagtacaagtcggattgatgttgagttgggtgccaaactcttccggcatttactggcgctaccgatctcttattttgagagtcgtcgtgttggtgatactgttgcgagggtaagagaattagaccagatccgtaattttctgacaggacaggcattaacatctgttttggacttattattttcactcatattttttgcggtaatgtggtattacagcccaaagcttactctggtgatcttattttcgctgccttgttatgctgcatggtctgtttttattagccccattttgcgacgtcgccttgatgataagttttcacggaatgcggataatcaatctttcctggtggaatcagtaacggcgattaacactataaaagctatggcagtctcacctcagatgacgaacatatgggacaaacaattggcaggatatgttgctgcaggctttaaagtgacagtattagcaaccattggtcaacaaggaatacagttaatacaaaagactgttatgatcatcaacctatggttgggagcacacctggttatttccggggatttaagtattggtcagttaattgcttttaatatgcttgctggtcagattgttgcaccggttattcgccttgcacaaatctggcaggatttccagcaggttggtatatcagttacccgccttggtgatgtgcttaactctccaactgaaagttatcatgggaaactgacattgccggaaattaatggtgatatcacttttcgtaatatccggtttcgctataaacctgattctccggttattttggacaatatcaatcttagtattaagcagggggaggttattggtattgtcggacgttctggttcaggaaaaagcacattaactaaattaattcaacgtttttatattcctgaaaatggccaggtattaattgatggacatgatcttgcgttggctgatcctaactggttacgtcgtcaggtgggggttgtgttgcaggacaatgtgctgcttaatcgcagtattattgataatatttcactggctaatcctggcatgtccgtcgaaaaagttatttatgcagcgaaattagcaggcgctcatgattttatttctgatttgcgtgaggggtataacaccattgtcggggaacagggggcaggattatccggaggtcaacgtcaacgcatcgcaattgcaagggcgctggtgaacaaccctaaaatactcatttttgatgaagcaaccagtgctctggattatgagtcggagcatgtcatcatgcgcaatatgcacaaaatatgtaagggcagaacggttataatcattgctcatcgtctgtctacagtaaaaaatgcagaccgcattattgtcatggaaaaagggaaaattgttgaacagggtaaacataaggagctgctttctgaaccggaaagtttatacagttacttatatcagttacagtcagactaacagaaagaacagaagaatatgaaaacatggttaatggggttcagcgagttcctgttgcgctataaacttgtctggagtgaaacatggaaaatccggaagcaattagatactccggtacgtgaaaaggacgaaaatgagttcttacccgctcatctggaattaattgaaacgccagtatccagacggccgcgtctggttgcttattttattatggggtttctggttattgcttttattttatctgttttaggccaagtggaaattgttgccactgcaaatgggaaattaacacacagtgggcgtagtaaagaaattaaacctattgaaaactcaatagttaaagaaattatcgtaaaagaaggagagtcagtccggaaaggggatgtgttattaaagcttacagcactgggagctgaagctgatacgttaaaaacacagtcatcactgttacaggccaggctggaacaaactcggtatcaaattctgagcaggtcaattgaattaaataaactacctgaactaaagcttcctgatgagccttattttcagaatgtatctgaagaggaagtactgcgtttaacttctttgataaaagaacagttttccacatggcaaaatcagaagtatcaaaaagaactgaatttggataagaaaagagcagagcgattaacagtacttgcccgtataaaccgttatgaaaatttatcaagggttgaaaaaagccgtctggatgatttcagtagtttattgcataaacaggcaattgcaaaacatgctgtacttgagcaggagaataaatatgtcgaagcagtaaatgaattacgagtttataaatcacaactggagcaaattgagagtgagatattgtctgcaaaagaagaatatcagcttgttacgcagctttttaaaaatgaaattttagataagctaagacaaacaacagacaacattgggttattaactctggaattagcgaaaaatgaagagcgtcaacaggcttcagtaatcagggccccagtttcgggaaaagttcagcaactgaaggttcatactgaaggtggggttgttacaacagcggaaacactgatggtcatcgttccggaagatgacacgctggaggttactgctctggtacaaaataaagatattggttttattaacgtcgggcagaatgccatcattaaagtggaggcatttccttatacacgatatggttatctggtgggtaaggtgaaaaatataaatttagatgcaatagaagaccagagactgggacttgtttttaatgttattatttctattgaagagaattgtttgtcaaccgggaataaaaacattccattaagctcgggtatggcagtcactgcagaaataaagacaggtatgcgaagtgtaatcagttatcttcttagtcctttagaagagtcagtaacagaaagtttacgtgagcgttaatactagtagcggccgctgcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K223055_sequence 1 gaattcgcggccgcttctagatgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggccgagaacctgatgcaagtttatcagcaagcacgccttagtaacccggaattgcgtaagtctgccgccgatcgtgatgctgcctttgaaaaaattaatgaagcgcgcagtccattactgccacagctaggtttaggtgcagattacacctatagcaacggctaccgcgacgcgaacggcatcaactctaacgcgaccagtgcgtccttgcagttaactcaatccatttttgatatgtcgaaatggcgtgcgttaacgctgcaggaaaaagcagcagggattcaggacgtcacgtatcagaccgatcagcaaaccttgatcctcaacaccgcgaccgcttatttcaacgtgttgaatgctattgacgttctttcctatacacaggcacaaaaagaagcgatctaccgtcaattagatcaaaccacccaacgttttaacgtgggcctggtagcgatcaccgacgtgcagaacgcccgcgcacagtacgataccgtgctggcgaacgaagtgaccgcacgtaataaccttgataacgcggtagagcagctgcgccagatcaccggtaactactatccggaactggctgcgctgaatgtcgaaaactttaaaaccgacaaaccacagccggttaacgcgctgctgaaagaagccgaaaaacgcaacctgtcgctgttacaggcacgcttgagccaggacctggcgcgcgagcaaattcgccaggcgcaggatggtcacttaccgactctggatttaacggcttctaccgggatttctgacacctcttatagcggttcgaaaacccgtggtgccgctggtacccagtatgacgatagcaatatgggccagaacaaagttggcctgagcttctcgctgccgatttatcagggcggaatggttaactcgcaggtgaaacaggcacagtacaactttgtcggtgccagcgagcaactggaaagtgcccatcgtagcgtcgtgcagaccgtgcgttcctccttcaacaacattaatgcatctatcagtagcattaacgcctacaaacaagccgtagtttccgctcaaagctcattagacgcgatggaagcgggctactcggtcggtacgcgtaccattgttgatgtgttggatgcgaccaccacgttgtacaacgccaagcaagagctggcgaatgcgcgttataactacctgattaatcagctgaatattaagtcagctctgggtacgttgaacgagcaggatctgctggcactgaacaatgcgctgagcaaaccggtttccactaatccggaaaacgttgcaccgcaaacgccggaacagaatgctattgctgatggttatgcgcctgatagcccggcaccagtcgttcagcaaacatccgcacgcactaccaccagtaacggtcataaccctttccgtaactgatactagtagcggccgctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z