BBa_K223062
type: ComponentDefinition
persistent identity: BBa K223062
display id: BBa_K223062
version: 1
was generated by: iGEM to SBOL conversion
description: TolC and Hemolysin B, C and D gene complex
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: Synthesized by Stanford iGEM 2009
owned by: james
owned by: myers
mutable description: This a composite part of BBa_K223055, BBa_K223056, BBa_K223057 that provides all necessary genes to create a channel protein in the extra cellular membrane of a prokaryotic cell. This channel can be used to export large organic molecules fused to a signal sequence, HlyAs
discontinued: false
dominant: false
group u list: _380_
m user id: 0
owner id: 4529
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Anusuya Ramasubramanian
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_K223055
component: BBa_K223056
component: BBa_B0010
component: BBa_K223057
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_K223055
sequence annotation: BBa_K223056
sequence annotation: BBa_K223057
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K223062_sequence/1

BBa_B0034
type: Component
persistent identity: component2051703
display id: component2051703
version: 1
top level: BBa_K223062
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component2051706
display id: component2051706
version: 1
top level: BBa_K223062
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component2051709
display id: component2051709
version: 1
top level: BBa_K223062
definition: BBa_B0034
access: public

BBa_K223055
type: Component
persistent identity: component2051704
display id: component2051704
version: 1
top level: BBa_K223062
definition: BBa_K223055
access: public

BBa_K223056
type: Component
persistent identity: component2051707
display id: component2051707
version: 1
top level: BBa_K223062
definition: BBa_K223056
access: public

BBa_B0010
type: Component
persistent identity: component2051711
display id: component2051711
version: 1
top level: BBa_K223062
definition: BBa_B0010
access: public

BBa_K223057
type: Component
persistent identity: component2051710
display id: component2051710
version: 1
top level: BBa_K223062
definition: BBa_K223057
access: public

BBa_B0012
type: Component
persistent identity: component2051713
display id: component2051713
version: 1
top level: BBa_K223062
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2051703
display id: annotation2051703
version: 1
top level: BBa_K223062
location: https://synbiohub.org/public/igem/BBa_K223062/annotation2051703/range2051703/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K223062/annotation2051703/range2051703/1
type: Range
persistent identity: range2051703
display id: range2051703
version: 1
top level: BBa_K223062
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K223055
type: SequenceAnnotation
persistent identity: annotation2051704
display id: annotation2051704
version: 1
top level: BBa_K223062
location: https://synbiohub.org/public/igem/BBa_K223062/annotation2051704/range2051704/1
component: BBa_K223055

https://synbiohub.org/public/igem/BBa_K223062/annotation2051704/range2051704/1
type: Range
persistent identity: range2051704
display id: range2051704
version: 1
top level: BBa_K223062
direction: direction/reverse
start: 21
end: 1543
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2051706
display id: annotation2051706
version: 1
top level: BBa_K223062
location: https://synbiohub.org/public/igem/BBa_K223062/annotation2051706/range2051706/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K223062/annotation2051706/range2051706/1
type: Range
persistent identity: range2051706
display id: range2051706
version: 1
top level: BBa_K223062
direction: direction/reverse
start: 1552
end: 1563
orientation: inline

BBa_K223056
type: SequenceAnnotation
persistent identity: annotation2051707
display id: annotation2051707
version: 1
top level: BBa_K223062
location: https://synbiohub.org/public/igem/BBa_K223062/annotation2051707/range2051707/1
component: BBa_K223056

https://synbiohub.org/public/igem/BBa_K223062/annotation2051707/range2051707/1
type: Range
persistent identity: range2051707
display id: range2051707
version: 1
top level: BBa_K223062
direction: direction/reverse
start: 1572
end: 4640
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2051709
display id: annotation2051709
version: 1
top level: BBa_K223062
location: https://synbiohub.org/public/igem/BBa_K223062/annotation2051709/range2051709/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K223062/annotation2051709/range2051709/1
type: Range
persistent identity: range2051709
display id: range2051709
version: 1
top level: BBa_K223062
direction: direction/reverse
start: 4649
end: 4660
orientation: inline

BBa_K223057
type: SequenceAnnotation
persistent identity: annotation2051710
display id: annotation2051710
version: 1
top level: BBa_K223062
location: https://synbiohub.org/public/igem/BBa_K223062/annotation2051710/range2051710/1
component: BBa_K223057

https://synbiohub.org/public/igem/BBa_K223062/annotation2051710/range2051710/1
type: Range
persistent identity: range2051710
display id: range2051710
version: 1
top level: BBa_K223062
direction: direction/reverse
start: 4669
end: 8361
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2051711
display id: annotation2051711
version: 1
top level: BBa_K223062
location: https://synbiohub.org/public/igem/BBa_K223062/annotation2051711/range2051711/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K223062/annotation2051711/range2051711/1
type: Range
persistent identity: range2051711
display id: range2051711
version: 1
top level: BBa_K223062
direction: direction/forward
start: 8370
end: 8449
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2051713
display id: annotation2051713
version: 1
top level: BBa_K223062
location: https://synbiohub.org/public/igem/BBa_K223062/annotation2051713/range2051713/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K223062/annotation2051713/range2051713/1
type: Range
persistent identity: range2051713
display id: range2051713
version: 1
top level: BBa_K223062
direction: direction/forward
start: 8458
end: 8498
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K223055
type: ComponentDefinition
persistent identity: BBa K223055
display id: BBa_K223055
version: 1
was generated by: iGEM to SBOL conversion
description: TolC gene
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: GenBank Escherichia coli str. K-12 substr. MG1655 http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.2/from=3176137&to=3177618&report=fasta Stanford iGEM Team 2009 altered the sequence restriction sites and unknown portions of the sequence.
owned by: james
owned by: myers
mutable description: TolC, along with HemolysinB, C, and D, for a channel in the cellular membrane to allow for the extracellular transport of HlyAs-fused protein sequences. The TolC receptor, in conjunction to the Hly genes, can serve as channel for transporting large proteins.
discontinued: false
dominant: false
group u list: _380_
m user id: 0
owner id: 4529
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Anusuya Ramasubramanian
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K223055_sequence/1

BBa_K223056
type: ComponentDefinition
persistent identity: BBa K223056
display id: BBa_K223056
version: 1
was generated by: iGEM to SBOL conversion
description: Front Flanking Sequence (HlyR, IS1, HlyC)
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: Compiled from several different papers: 1. Cloning of a cDNA Encoding an Aldehyde Dehydrogenase and Its Expression in Escherichia coli Xianshu Wang, Peter Penzes, and Joseph L. Napoli THE JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 271, No. 27, Issue of July 5, pp. 16288 ???16293, 1996 2. Escherichia coli CFT073 (GenBank) http://www.ncbi.nlm.nih.gov/nuccore/NC_004431.1/from=3418210&to=3418884&report=fasta
owned by: james
owned by: myers
mutable description: This front flanking sequence has three of the hemolysin parts - HlyR, Is1 and HlyC. In conjunction with rear flanking sequence, containing HlyB and HlyD, and TolC (part K223055), this part creates a channel protein to facilitate the extracellular export HlyAs-fused proteins.
discontinued: false
dominant: false
group u list: _380_
m user id: 0
owner id: 4529
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Anusuya Ramasubramanian
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K223056_sequence/1

BBa_K223057
type: ComponentDefinition
persistent identity: BBa K223057
display id: BBa_K223057
version: 1
was generated by: iGEM to SBOL conversion
description: Rear Flanking Sequence (HlyB and HlyD)
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:31Z
mutable provenance: Compiled from several different sources: 1. GenBank E. coli str. CFT073 http://www.ncbi.nlm.nih.gov/nuccore/NC_004431.1/from=3422041&to=3424164&report=fasta 2. GenBank E. coli str. CFT073 http://www.ncbi.nlm.nih.gov/nuccore/NC_004431.1/from=3424183&to=3425619&report=fasta
owned by: james
owned by: myers
mutable description: Along with parts BBa_K223055 and K223056, this part helps form the channel protein for the extracellular transport of HlyAs-fused protein.
discontinued: false
dominant: false
group u list: _380_
m user id: 0
owner id: 4529
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Stanford iGEM Team 2009 altered sequences for internal restriction sites
star: false
creator: Anusuya Ramasubramanian
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K223057_sequence/1

https://synbiohub.org/public/igem/BBa_K223062_sequence/1
type: Sequence
persistent identity: BBa K223062 sequence
display id: BBa_K223062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagaggaattcgcggccgcttctagatgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggccgagaacctgatgcaagtttatcagcaagcacgccttagtaacccggaattgcgtaagtctgccgccgatcgtgatgctgcctttgaaaaaattaatgaagcgcgcagtccattactgccacagctaggtttaggtgcagattacacctatagcaacggctaccgcgacgcgaacggcatcaactctaacgcgaccagtgcgtccttgcagttaactcaatccatttttgatatgtcgaaatggcgtgcgttaacgctgcaggaaaaagcagcagggattcaggacgtcacgtatcagaccgatcagcaaaccttgatcctcaacaccgcgaccgcttatttcaacgtgttgaatgctattgacgttctttcctatacacaggcacaaaaagaagcgatctaccgtcaattagatcaaaccacccaacgttttaacgtgggcctggtagcgatcaccgacgtgcagaacgcccgcgcacagtacgataccgtgctggcgaacgaagtgaccgcacgtaataaccttgataacgcggtagagcagctgcgccagatcaccggtaactactatccggaactggctgcgctgaatgtcgaaaactttaaaaccgacaaaccacagccggttaacgcgctgctgaaagaagccgaaaaacgcaacctgtcgctgttacaggcacgcttgagccaggacctggcgcgcgagcaaattcgccaggcgcaggatggtcacttaccgactctggatttaacggcttctaccgggatttctgacacctcttatagcggttcgaaaacccgtggtgccgctggtacccagtatgacgatagcaatatgggccagaacaaagttggcctgagcttctcgctgccgatttatcagggcggaatggttaactcgcaggtgaaacaggcacagtacaactttgtcggtgccagcgagcaactggaaagtgcccatcgtagcgtcgtgcagaccgtgcgttcctccttcaacaacattaatgcatctatcagtagcattaacgcctacaaacaagccgtagtttccgctcaaagctcattagacgcgatggaagcgggctactcggtcggtacgcgtaccattgttgatgtgttggatgcgaccaccacgttgtacaacgccaagcaagagctggcgaatgcgcgttataactacctgattaatcagctgaatattaagtcagctctgggtacgttgaacgagcaggatctgctggcactgaacaatgcgctgagcaaaccggtttccactaatccggaaaacgttgcaccgcaaacgccggaacagaatgctattgctgatggttatgcgcctgatagcccggcaccagtcgttcagcaaacatccgcacgcactaccaccagtaacggtcataaccctttccgtaactgatactagtagcggccgctgcagtactagagaaagaggagaaatactagaggaattcgcggccgcttctagaggaattccaagcgaagtccatccccctccctcttgattacaagggtgataattattattcgcatttgtgtggtaatgggatagaaaggaatggatagaaaaagaacaaaattagtatagcaatagatatgcccactgcattgaatacttacagggcattattttattatgtttaaattgaagtggtctctggtttgatttatttgttattcaagggggctgtttggagatcggaaaattctgtacgttaagtgtattatttaaccagtttcgatgcgtaacagattgattttgcgtcagcggttatcgcttttaagttgttgctcttgcgctatcgcgtttaggttatccgattaaagtcaaatttcctgaaaatgctgtatagcgcgggagtgcaccttatagctgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223055_sequence/1
type: Sequence
persistent identity: BBa K223055 sequence
display id: BBa_K223055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagatgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggccgagaacctgatgcaagtttatcagcaagcacgccttagtaacccggaattgcgtaagtctgccgccgatcgtgatgctgcctttgaaaaaattaatgaagcgcgcagtccattactgccacagctaggtttaggtgcagattacacctatagcaacggctaccgcgacgcgaacggcatcaactctaacgcgaccagtgcgtccttgcagttaactcaatccatttttgatatgtcgaaatggcgtgcgttaacgctgcaggaaaaagcagcagggattcaggacgtcacgtatcagaccgatcagcaaaccttgatcctcaacaccgcgaccgcttatttcaacgtgttgaatgctattgacgttctttcctatacacaggcacaaaaagaagcgatctaccgtcaattagatcaaaccacccaacgttttaacgtgggcctggtagcgatcaccgacgtgcagaacgcccgcgcacagtacgataccgtgctggcgaacgaagtgaccgcacgtaataaccttgataacgcggtagagcagctgcgccagatcaccggtaactactatccggaactggctgcgctgaatgtcgaaaactttaaaaccgacaaaccacagccggttaacgcgctgctgaaagaagccgaaaaacgcaacctgtcgctgttacaggcacgcttgagccaggacctggcgcgcgagcaaattcgccaggcgcaggatggtcacttaccgactctggatttaacggcttctaccgggatttctgacacctcttatagcggttcgaaaacccgtggtgccgctggtacccagtatgacgatagcaatatgggccagaacaaagttggcctgagcttctcgctgccgatttatcagggcggaatggttaactcgcaggtgaaacaggcacagtacaactttgtcggtgccagcgagcaactggaaagtgcccatcgtagcgtcgtgcagaccgtgcgttcctccttcaacaacattaatgcatctatcagtagcattaacgcctacaaacaagccgtagtttccgctcaaagctcattagacgcgatggaagcgggctactcggtcggtacgcgtaccattgttgatgtgttggatgcgaccaccacgttgtacaacgccaagcaagagctggcgaatgcgcgttataactacctgattaatcagctgaatattaagtcagctctgggtacgttgaacgagcaggatctgctggcactgaacaatgcgctgagcaaaccggtttccactaatccggaaaacgttgcaccgcaaacgccggaacagaatgctattgctgatggttatgcgcctgatagcccggcaccagtcgttcagcaaacatccgcacgcactaccaccagtaacggtcataaccctttccgtaactgatactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223056_sequence/1
type: Sequence
persistent identity: BBa K223056 sequence
display id: BBa_K223056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagaggaattccaagcgaagtccatccccctccctcttgattacaagggtgataattattattcgcatttgtgtggtaatgggatagaaaggaatggatagaaaaagaacaaaattagtatagcaatagatatgcccactgcattgaatacttacagggcattattttattatgtttaaattgaagtggtctctggtttgatttatttgttattcaagggggctgtttggagatcggaaaattctgtacgttaagtgtattatttaaccagtttcgatgcgtaacagattgattttgcgtcagcggttatcgcttttaagttgttgctcttgcgctatcgcgtttaggttatccgattaaagtcaaatttcctgaaaatgctgtatagcgcgggagtgcaccttatagctgtaggtaagtatgttcaaaaaatagtcttgccgtacaataattttccatatccaaactcactccttcaagattctggtcccggtttacgggtagtttccggaagggcggtagcatgctgattcaaactgcaagatgaaacattgtcggagttggatggaattaagtcatggctatagcatttgggcgtgcataacaaaattggtcctcatattttagagtatgattgcatattcactaatatttttactttctgatgcgtggtggcatcatgctttatgagataaacaatcctggtagactagccccctgaatctccagacaaccaatatcacttatttaagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttaggatcggagaaacgcagacggcgtactacacaggaaaagatcgctatcgttcagcagagctttgaaccgggaatgacggtctcccttgttgcccggcaacatggtgtggcagccagccagctatttctctggcgcaagcaataccaggagggaagtcttactgctgtggctgccggagaacaggtcgttcctgcctctgaacttgctgccgccatgaagcagattaaagagctccagcgcctgctcggcaaaaaaacgatggaaaatgaactccttaaagaagccgttgaatatgggcgagcaaaaaagtggatagcgcacgcgcccttattgcccggggatggggagtaagcttcgtcagccgttgtctccgggtgtcgcgtgcgcagttgcacgtcattctcagacgaaccgatgactggaaggacggccgccgcagccgtcacacggatgatacggatgtgcttcgccgtatacatcatgttatcggagagctgcccacatatggttatcgtcgggtatgggcgctgcttcgcagacaaacagaacttgatggtatgcctgcgatcaatgccaaatgtgtttaccggatcatgtgccagaatgcgctgttgcttgagcgaaaacccgctgtaccgccatcgaaacgggcacataccggcagagtggctgtgaaagaaagtaatcagcgatggtgctctgacgggtttgagttccgctgtgataacggagaaaaactgcgggtcacgttcgcgctggactgctgtgatcgtgaggcactgcactgggcggtcacaacgggtggcttcaacagtgaaacagtacaggacgtcatgctgggagcagtggaacgccgctttggcagcgagcttccggcgtctccagtggagtggctgacggataatggttcatgctaccgggcgaatgaaacacgtcagttcgccaggatgttgggacttgaaccgaagaacacggcagtgcggagtccggagagtaacggaataacagagagcttcgtgaaaacgataaagcgtgattacataagtatcatgcccaaaccagacgggttaacggcagcaaagaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K223057_sequence/1
type: Sequence
persistent identity: BBa K223057 sequence
display id: BBa_K223057_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagagtatattaatttaaatgatagcaatcttactgggctgtgccacataagattgctattttttttggagtcataatggattcttgtcataaaattgattatgggttatacgccctggagattttagcccaataccataacgtctctgttaacccggaagaaattaaacatagatttgatacagacgggacaggtctgggattaacgtcatggttgcttgctgcgaaatctttagaactaaaggtaaaacaggtaaaaaaaacaattgatcgattaaactttatttttctgcccgcattagtctggagagaggatggacgtcattttattctgactaaaatcagcaaagaagtaaacagatatcttatttttgatttggagcagcgaaatccccgtgttctcgaacagtctgagtttgaggcgttatatcaggggcatattattcttattacttcccgttcttctgttaccgggaaactggcaaaatttgactttacctggtttattcctgccattataaaatacaggagaatatttattgaaacccttgttgtatctgtttttttacaattatttgcattaataacccccctttttttccaggtggttatggacaaagtattagtgcacagggggttttcaacccttaatgttattactgttgcattatctgttgtagtggtgtttgagattatactcagcggtttaagaacttacatttttgcacatagtacaagtcggattgatgttgagttgggtgccaaactcttccggcatttactggcgctaccgatctcttattttgagagtcgtcgtgttggtgatactgttgcgagggtaagagaattagaccagatccgtaattttctgacaggacaggcattaacatctgttttggacttattattttcactcatattttttgcggtaatgtggtattacagcccaaagcttactctggtgatcttattttcgctgccttgttatgctgcatggtctgtttttattagccccattttgcgacgtcgccttgatgataagttttcacggaatgcggataatcaatctttcctggtggaatcagtaacggcgattaacactataaaagctatggcagtctcacctcagatgacgaacatatgggacaaacaattggcaggatatgttgctgcaggctttaaagtgacagtattagcaaccattggtcaacaaggaatacagttaatacaaaagactgttatgatcatcaacctatggttgggagcacacctggttatttccggggatttaagtattggtcagttaattgcttttaatatgcttgctggtcagattgttgcaccggttattcgccttgcacaaatctggcaggatttccagcaggttggtatatcagttacccgccttggtgatgtgcttaactctccaactgaaagttatcatgggaaactgacattgccggaaattaatggtgatatcacttttcgtaatatccggtttcgctataaacctgattctccggttattttggacaatatcaatcttagtattaagcagggggaggttattggtattgtcggacgttctggttcaggaaaaagcacattaactaaattaattcaacgtttttatattcctgaaaatggccaggtattaattgatggacatgatcttgcgttggctgatcctaactggttacgtcgtcaggtgggggttgtgttgcaggacaatgtgctgcttaatcgcagtattattgataatatttcactggctaatcctggcatgtccgtcgaaaaagttatttatgcagcgaaattagcaggcgctcatgattttatttctgatttgcgtgaggggtataacaccattgtcggggaacagggggcaggattatccggaggtcaacgtcaacgcatcgcaattgcaagggcgctggtgaacaaccctaaaatactcatttttgatgaagcaaccagtgctct…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z