LOCUS       BBa_K225001             3929 bp    DNA     linear       16-Dec-2024
DEFINITION  Green light activated actuator
ACCESSION   BBa_K225001
VERSION     BBa_K225001.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    745..769
                     /label=Help:Barcodes
     CDS             19..769
                     /label=BBa_I15008
     misc_feature    782..785
                     /label=conserved
     RBS             778..789
                     /label=BBa_B0034
     CDS             796..1545
                     /label=PcyA
     misc_feature    1546..1570
                     /label=Help:Barcodes
     CDS             796..1570
                     /label=BBa_I15009
     misc_feature    1583..1586
                     /label=conserved
     RBS             1579..1590
                     /label=BBa_B0034
     CDS             1597..3792
                     /label=BBa_K225000
     stem_loop       3812..3855
                     /label=stem_loop
     misc_feature    3801..3880
                     /label=BBa_B0010
     terminator      3801..3880
                     /label=BBa_B0010
     stem_loop       3896..3915
                     /label=T7 TE
     misc_feature    3922..3922
                     /label=stop
     polyA_site      3916..3929
                     /label=polya
     misc_feature    3889..3929
                     /label=BBa_B0012
     terminator      3889..3929
                     /label=BBa_B0012
     terminator      3801..3929
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gagtgtcaac ttagcttccc agttgcggga agggacgaaa
       61 aaatcccact ccatggcgga gaacgtcggc tttgtcaaat gcttcctcaa gggcgttgtc
      121 gagaaaaatt cctaccgtaa gctggttggc aatctctact ttgtctacag tgccatggaa
      181 gaggaaatgg caaaatttaa ggaccatccc atcctcagcc acatttactt ccccgaactc
      241 aaccgcaaac aaagcctaga gcaagacctg caattctatt acggctccaa ctggcggcaa
      301 gaagtgaaaa tttctgccgc tggccaagcc tatgtggacc gagtccggca agtggccgct
      361 acggcccctg aattgttggt ggcccattcc tacacccgtt acctggggga tctttccggc
      421 ggtcaaattc tcaagaaaat tgcccaaaat gccatgaatc tccacgatgg tggcacagct
      481 ttctatgaat ttgccgacat tgatgacgaa aaggctttta aaaataccta ccgtcaagct
      541 atgaatgatc tgcccattga ccaagccacc gccgaacgga ttgtggatga agccaatgac
      601 gcctttgcca tgaacatgaa aatgttcaac gaacttgaag gcaacctgat caaggcgatc
      661 ggcattatgg tgttcaacag cctcacccgt cgccgcagtc aaggcagcac cgaagttggc
      721 ctcgccacct ccgaaggcta ataacgctga tagtgctagt gtagatcgct actagagaaa
      781 gaggagaaat actagatggc cgtcactgat ttaagtttga ccaattcttc cctgatgcct
      841 acgttgaacc cgatgattca acagttggcc ctggcgatcg ccgctagttg gcaaagttta
      901 cccctcaagc cctatcaatt gccggaggat ttgggctacg tagaaggccg cctggaaggg
      961 gaaaagttag tgattgaaaa tcggtgctac caaacgcccc agtttcgcaa aatgcatttg
     1021 gagttggcca aggtgggcaa agggttggat attctccact gtgtaatgtt tcctgagcct
     1081 ttatacggtc tacctttgtt tggctgtgac attgtggccg gccccggtgg agtaagtgcg
     1141 gctattgcgg atctatcccc cacccaaagc gatcgccaat tgcccgcagc gtaccaaaaa
     1201 tcattggcag agctaggcca gccagaattt gagcaacaac gggaattgcc cccctgggga
     1261 gaaatatttt ctgaatattg tttattcatc cgtcccagca atgtcactga agaagaaaga
     1321 tttgtacaaa gggtagtgga ctttttgcaa attcattgtc accaatccat cgttgccgaa
     1381 cccttgtctg aagctcaaac tttggagcac cgtcaggggc aaattcatta ctgccaacaa
     1441 caacagaaaa atgataaaac ccgtcgggta ctggaaaaag cttttgggga agcttgggcg
     1501 gaacggtata tgagccaagt cttatttgat gttatccaat aataactctg atagtgctag
     1561 tgtagatctc tactagagaa agaggagaaa tactagatgg gaaaatttct gatcccgatc
     1621 gagtttgtgt tcctggcaat cgccatgacc tgttatctgt ggcatcgtca aaaccaagaa
     1681 cgtcgccgta ttgagattag cattaaacag cagacccagc gtgagcgctt tatcaaccag
     1741 atcactcagc atatccgtca gtccctgaac ctggaaaccg tcctgaatac caccgttgcc
     1801 gaagtgaaaa cactgctgca agtggaccgt gtcctgattt atcgtatttg gcaagatggc
     1861 accggaagtg ctattaccga atccgtgaac gccaattatc cgagcattct gggccgtacc
     1921 ttttctgatg aggtgttccc ggtcgaatat caccaggcct ataccaaagg caaagttcgt
     1981 gctatcaacg acattgacca agacgacatc gaaatctgtc tggccgattt tgtaaaacag
     2041 ttcggggtga aatccaaact ggtggtgcct attctgcaac acaatcgtgc ctcaagcctg
     2101 gataacgaaa gcgagttccc gtatctgtgg ggactgctga tcacacatca gtgtgccttc
     2161 acacgtccat ggcaaccatg ggaggttgag ctgatgaaac agctagccaa tcaggttgcc
     2221 attgccattc aacagtccga gctgtatgaa caactgcaac aactgaacaa agacctggaa
     2281 aaccgtgtgg agaaacgtac acaacaactg gcagccacca atcaatctct gcgcatggaa
     2341 atcagcgaac gccagaaaac tgaggcagca ctgcgccata ctaaccatac cctgcaaagt
     2401 ctgatcgccg cttcacctcg tggcattttt actctgaatc tggctgacca aatccagatc
     2461 tggaatccga cagcagagcg tatctttggt tggaccgaga cagaaatcat tgctcacccg
     2521 gaactgctga caagcaacat cctgctggag gactatcagc aattcaaaca aaaagtgctg
     2581 tccggcatgg tttcaccaag tctggagctg aaatgtcaga aaaaagacgg ctcgtggatc
     2641 gaaattgttc tgagcgctgc cccgctgctg gactctgaag aaaacattgc cggtctggtt
     2701 gctgtggtag ccgatattac cgagcagaaa cgtcaggctg aacaaatccg cctgctgcaa
     2761 agtgtggttg tgaacaccaa cgatgctgta gtgatcaccg aagctgaacc gatcgatgac
     2821 cccgggcctc gtatcctgta tgtgaacgaa gccttcacga aaatcacggg ctataccgcc
     2881 gaagaaatgc tgggcaaaac acctcgtgtc ctgcaagggc ccaaaacatc gcgaaccgaa
     2941 ctggaccgtg tccgtcaagc cattagtcag tggcagtctg tgacagtgga agtcatcaac
     3001 tatcgtaaag atgggagcga gttttgggtg gaattttccc tggttccagt ggccaacaaa
     3061 acgggctttt atacccattg gattgccgta caacgtgatc atatggcggc tggtgttaag
     3121 caactggcgg atgaccgcac gctgctgatg gcgggggtaa gtcacgactt gcgcacgccg
     3181 ctgacgcgta ttcgcctggc gactgagatg atgagcgagc aggatggcta tctggcagaa
     3241 tcgatcaata aagatatcga agagtgcaac gccatcattg agcagtttat cgactacctg
     3301 cgcaccgggc aggagatgcc gatggaaatg gcggatctta atgcagtact cggtgaggtg
     3361 attgctgccg aaagtggcta tgagcgggaa attgaaaccg cgctttaccc cggcagcatt
     3421 gaagtgaaaa tgcacccgct gtcgatcaaa cgcgcggtgg cgaatatggt ggtcaacgcc
     3481 gcccgttatg gcaatggctg ggtcaaagtc agcagcggaa cggagccgaa tcgcgcctgg
     3541 ttccaggtgg aagatgacgg tccgggaatt gcgccggaac aacgtaagca cctgttccag
     3601 ccgtttgtcc gcggcgacag tgcgcgcacc attagcggca cgggattagg gctggcaatt
     3661 gtgcagcgta tcgtggataa ccataacggg atgctggagc ttggcaccag cgagcggggc
     3721 gggctttcca ttcgcgcctg gctgccagtg ccggtaacgc gggcgcaggg catgacaaaa
     3781 gaagggtaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     3841 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     3901 accttcgggt gggcctttct gcgtttata
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