BBa_K225000 1 GlrN Green light receptor 2009-10-14T11:00:00Z 2015-05-08T01:11:31Z Synechocystis sp. PCC 6803 a green-light responsive fusion protein (ScaS/EnvZ fusion) false false _324_ 0 4689 9 It's complicated true The fusion of a green-ight responsive domain of ScaS and EnvZ false Nasa SAVORY BBa_I15009 1 BBa_I15009 phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis 2004-09-19T11:00:00Z 2015-08-31T04:07:38Z Voigt Lab via Anselm Levskaya Released HQ 2013 Second of two required phycocyanobilin biosynthetic genes. false false _5_ 0 88 7 In stock true true Jeff Tabor annotation1891612 1 PcyA range1891612 1 1 750 annotation2214032 1 Help:Barcodes range2214032 1 751 775 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K225001 1 BBa_K225001 Green light activated actuator 2009-10-14T11:00:00Z 2015-05-08T01:11:31Z ScaS from Synechocysitis sp. PCC 6803 RBS-ho1-RBS-pcyA-RBS-GlrN-Trem false false _324_ 0 4689 9 It's complicated true ScaS/EnvZ fusion was designed by combining the light receptor domain of ScaS (phytochrome from Synechocystis sp PCC 6803) and EnvZ. I mean that I changed the kinase domain of ScaS to EnvZ to make it work in E coli. false Nasa SAVORY component2272049 1 BBa_B0034 component2272059 1 BBa_K225000 component2272051 1 BBa_I15008 component2272053 1 BBa_B0034 component2272056 1 BBa_I15009 component2272058 1 BBa_B0034 component2272066 1 BBa_B0015 annotation2272051 1 BBa_I15008 range2272051 1 19 769 annotation2272056 1 BBa_I15009 range2272056 1 796 1570 annotation2272053 1 BBa_B0034 range2272053 1 778 789 annotation2272066 1 BBa_B0015 range2272066 1 3801 3929 annotation2272059 1 BBa_K225000 range2272059 1 1597 3792 annotation2272049 1 BBa_B0034 range2272049 1 1 12 annotation2272058 1 BBa_B0034 range2272058 1 1579 1590 BBa_I15008 1 BBa_I15008 heme oxygenase (ho1) from Synechocystis 2004-09-19T11:00:00Z 2015-08-31T04:07:38Z Voigt Lab via Anselm Levskaya Released HQ 2013 One of two requisite genes required for the biosynthesis of phycocyanobilin from heme. false false _5_ 0 88 7 In stock true true Jeff Tabor annotation2214026 1 Help:Barcodes range2214026 1 727 751 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K225000_sequence 1 atgggaaaatttctgatcccgatcgagtttgtgttcctggcaatcgccatgacctgttatctgtggcatcgtcaaaaccaagaacgtcgccgtattgagattagcattaaacagcagacccagcgtgagcgctttatcaaccagatcactcagcatatccgtcagtccctgaacctggaaaccgtcctgaataccaccgttgccgaagtgaaaacactgctgcaagtggaccgtgtcctgatttatcgtatttggcaagatggcaccggaagtgctattaccgaatccgtgaacgccaattatccgagcattctgggccgtaccttttctgatgaggtgttcccggtcgaatatcaccaggcctataccaaaggcaaagttcgtgctatcaacgacattgaccaagacgacatcgaaatctgtctggccgattttgtaaaacagttcggggtgaaatccaaactggtggtgcctattctgcaacacaatcgtgcctcaagcctggataacgaaagcgagttcccgtatctgtggggactgctgatcacacatcagtgtgccttcacacgtccatggcaaccatgggaggttgagctgatgaaacagctagccaatcaggttgccattgccattcaacagtccgagctgtatgaacaactgcaacaactgaacaaagacctggaaaaccgtgtggagaaacgtacacaacaactggcagccaccaatcaatctctgcgcatggaaatcagcgaacgccagaaaactgaggcagcactgcgccatactaaccataccctgcaaagtctgatcgccgcttcacctcgtggcatttttactctgaatctggctgaccaaatccagatctggaatccgacagcagagcgtatctttggttggaccgagacagaaatcattgctcacccggaactgctgacaagcaacatcctgctggaggactatcagcaattcaaacaaaaagtgctgtccggcatggtttcaccaagtctggagctgaaatgtcagaaaaaagacggctcgtggatcgaaattgttctgagcgctgccccgctgctggactctgaagaaaacattgccggtctggttgctgtggtagccgatattaccgagcagaaacgtcaggctgaacaaatccgcctgctgcaaagtgtggttgtgaacaccaacgatgctgtagtgatcaccgaagctgaaccgatcgatgaccccgggcctcgtatcctgtatgtgaacgaagccttcacgaaaatcacgggctataccgccgaagaaatgctgggcaaaacacctcgtgtcctgcaagggcccaaaacatcgcgaaccgaactggaccgtgtccgtcaagccattagtcagtggcagtctgtgacagtggaagtcatcaactatcgtaaagatgggagcgagttttgggtggaattttccctggttccagtggccaacaaaacgggcttttatacccattggattgccgtacaacgtgatcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataa BBa_K225001_sequence 1 aaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgggaaaatttctgatcccgatcgagtttgtgttcctggcaatcgccatgacctgttatctgtggcatcgtcaaaaccaagaacgtcgccgtattgagattagcattaaacagcagacccagcgtgagcgctttatcaaccagatcactcagcatatccgtcagtccctgaacctggaaaccgtcctgaataccaccgttgccgaagtgaaaacactgctgcaagtggaccgtgtcctgatttatcgtatttggcaagatggcaccggaagtgctattaccgaatccgtgaacgccaattatccgagcattctgggccgtaccttttctgatgaggtgttcccggtcgaatatcaccaggcctataccaaaggcaaagttcgtgctatcaacgacattgaccaagacgacatcgaaatctgtctggccgattttgtaaaacagttcggggtgaaatccaaactggtggtgcctattctgcaacacaatcgtgcctcaagcctggataacgaaagcgagttcccgtatctgtggggactgctgatcacacatcagtgtgccttcacacgtccatggcaaccatgggaggttgagctgatgaaacagctagccaatcaggttgccattgccattcaacagtccgagctgtatgaacaactgcaacaactgaacaaagacctggaaaaccgtgtggagaaacgtacacaacaactggcagccaccaatcaatctctgcgcatggaaatcagcgaacgccagaaaactgaggcagcactgcgccatactaaccataccctgcaaagtctgatcgccgcttcacctcgtggcatttttactctgaatctggctgaccaaatccagatctggaatccgacagcagagcgtatctttggttggaccgagacagaaatcattgctcacccggaactgctgacaagcaacatcctgctggaggactatcagcaattcaaacaaaaagtgctgtccggcatggtttcaccaagtctggagctgaaatgtcagaaaaaagacggctcgtggatcgaaattgttctgagcgctgccccgctgctggactctgaagaaaacattgccggtctggttgctgtggtagccgatattaccgagcagaaacgtcaggctgaacaaatccgcctgctgcaaagtgtggttgtgaacaccaacgatgctgtagtgatcaccgaagctgaaccgatcgatgaccccgggcctcgtatcctgtatgtgaacgaagccttcacgaaaatcacgggctataccgccgaagaaatgctgggcaaaacacctcgtgtcctgcaagggcccaaaacatcgcgaaccgaactggaccgtgtccgtcaagccattagtcagtggcagtctgtgacagtggaagtcatcaactatcgtaaagatgggagcgagttttgggtggaattttccctggttccagtggccaacaaaacgggcttttatacccattggattgccgtacaacgtgatcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_I15009_sequence 1 atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctc BBa_I15008_sequence 1 atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z