LOCUS       BBa_K227012             1375 bp    DNA     linear       22-Nov-2024
DEFINITION  RBS34+OmpR(sphaeroides)+Term+ompC+pucBA
ACCESSION   BBa_K227012
VERSION     BBa_K227012.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=ompR(sph)
     CDS             19..738
                     /label=BBa_K227010
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     misc_feature    884..991
                     /label=BBa_R0082
     misc_feature    896..913
                     /label=C1 OmpR
     misc_feature    917..934
                     /label=C2 OmpR
     misc_feature    937..954
                     /label=C3 OmpR
     promoter        958..963
                     /label=-35
     promoter        981..986
                     /label=-10
     misc_feature    1027..1031
                     /label=RBS
     CDS             1040..1194
                     /label=PucB
     misc_feature    1040..1375
                     /label=BBa_K227006
     misc_feature    1198..1202
                     /label=RBS
     CDS             1211..1375
                     /label=pucA
     misc_feature    884..1375
                     /label=BBa_K227008
ORIGIN
        1 aaagaggaga aatactagat gcaggagaac tataagatcc tggtggtgga cgacgacatg
       61 cgcctgcgcg ccctgctgga gcgctacctg accgagcagg gcttccaggt gcgctcggtg
      121 gccaacgccg agcagatgga ccgcctgctg acccgcgagt cgttccatct gatggtgctg
      181 gacctgatgc tgccgggcga ggacggcctg tcgatctgcc gccgcctgcg ctcgcagtcg
      241 aacccgatgc cgatcatcat ggtgaccgcc aagggcgagg aggtggaccg catcgtgggc
      301 ctggagatcg gcgccgacga ctacatcccg aagccgttca acccgcgcga gctgctggcc
      361 cgcatccgcg ccgtgctgcg ccgccaggcc aacgagctgc cgggcgcccc gtcgcaggag
      421 gaggccgtga tcgccttcgg caagttcaag ctgaacctgg gcacccgcga gatgttccgc
      481 gaggacgagc cgatgccgct gacctcgggc gagttcgccg tgctgaaggc cctggtgtcg
      541 catccgcgcg agccgctgtc gcgcgacaag ctgatgaacc tggcccgcgg ccgcgagtat
      601 tcggccatgg agcgctcgat cgacgtgcag atctcgcgcc tgcgccgcat ggtggaggag
      661 gacccggccc acccgcgcta catccagacc gtgtggggcc tgggctacgt gttcgtgccg
      721 gacggctcga aggcctgata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtcccttg catttacatt
      901 ttgaaacatc tatagcgata aatgaaacat cttaaaagtt ttagtatcat attcgtgttg
      961 gattattctg catttttggg gagaatggac ttactagagt acacgcaaac cgtcgattta
     1021 ccagttggga gacgacacag tgactgacga tctgaacaaa gtctggccga gcggcctgac
     1081 cgttgccgaa gccgaagaag ttcataagca actcatcctc ggcacccgcg tcttcggtgg
     1141 catggcgctc atcgcgcact tcctcgccgc cgctgcgacc ccgtggctcg gctgatagga
     1201 gaagactgac atgaccaacg gcaaaatctg gctcgtggtg aaaccgaccg tcggcgttcc
     1261 gctgttcctc agcgctgccg tcatcgcctc cgtcgttatc cacgctgctg tgctgacgac
     1321 caccacctgg ctgcccgcct actaccaagg ctcggctgcg gtcgcggccg agtaa
//