BBa_K227008 1 BBa_K227008 ompC Promoter+pucBA 2009-10-16T11:00:00Z 2015-05-08T01:11:32Z existing registry parts pucBA coding region from R. sphaeroides under regulation of ompC promotor (E.coli). false false _326_ 0 4645 9 It's complicated false no evidence of ompC region having a natural binding promotor in R. sphaeroides false WashU iGEM 2009 annotation2057770 1 C1 OmpR range2057770 1 13 30 annotation2057779 1 pucA range2057779 1 328 492 annotation2057769 1 BBa_R0082 range2057769 1 1 108 annotation2057773 1 -35 range2057773 1 75 80 annotation2057771 1 C2 OmpR range2057771 1 34 51 annotation2057772 1 C3 OmpR range2057772 1 54 71 annotation2057778 1 RBS range2057778 1 315 319 annotation2057775 1 RBS range2057775 1 144 148 annotation2057776 1 PucB range2057776 1 157 311 annotation2057777 1 BBa_K227006 range2057777 1 157 492 annotation2057774 1 -10 range2057774 1 98 103 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K227012 1 BBa_K227012 RBS34+OmpR(sphaeroides)+Term+ompC+pucBA 2009-10-16T11:00:00Z 2015-05-08T01:11:32Z composite part, synthesized in two fragments (RBS-Term, ompC+pucBA) from Geneart. Sequences from registry parts. intermediate part, used in conjunction with Cph8 to control LHII production in R. sphaeroides. false false _326_ 0 4645 9 It's complicated true ompR(sph) is codon-optimized for use in R. sphaeroides. false WashU iGEM 2009 component2057783 1 BBa_K227010 component2057781 1 BBa_B0034 component2057786 1 BBa_B0012 component2057784 1 BBa_B0010 component2057801 1 BBa_K227008 annotation2057786 1 BBa_B0012 range2057786 1 835 875 annotation2057781 1 BBa_B0034 range2057781 1 1 12 annotation2057784 1 BBa_B0010 range2057784 1 747 826 annotation2057783 1 BBa_K227010 range2057783 1 19 738 annotation2057801 1 BBa_K227008 range2057801 1 884 1375 BBa_K227010 1 BBa_K227010 OmpR (sphaeroides) 2009-10-16T11:00:00Z 2015-05-08T01:11:32Z sequence modified from part BBa_(((())))) OmpR promotor from E.coli, with DNA codon usage optimized for use in Rhodobacter sphaeroides. false false _326_ 0 4645 9 Not in stock false codon usage optimized for Rhodobacter sphaeroides using GeneDesigner. false WashU iGEM 2009 annotation2056947 1 ompR(sph) range2056947 1 1 720 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K227008_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagtacacgcaaaccgtcgatttaccagttgggagacgacacagtgactgacgatctgaacaaagtctggccgagcggcctgaccgttgccgaagccgaagaagttcataagcaactcatcctcggcacccgcgtcttcggtggcatggcgctcatcgcgcacttcctcgccgccgctgcgaccccgtggctcggctgataggagaagactgacatgaccaacggcaaaatctggctcgtggtgaaaccgaccgtcggcgttccgctgttcctcagcgctgccgtcatcgcctccgtcgttatccacgctgctgtgctgacgaccaccacctggctgcccgcctactaccaaggctcggctgcggtcgcggccgagtaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K227012_sequence 1 aaagaggagaaatactagatgcaggagaactataagatcctggtggtggacgacgacatgcgcctgcgcgccctgctggagcgctacctgaccgagcagggcttccaggtgcgctcggtggccaacgccgagcagatggaccgcctgctgacccgcgagtcgttccatctgatggtgctggacctgatgctgccgggcgaggacggcctgtcgatctgccgccgcctgcgctcgcagtcgaacccgatgccgatcatcatggtgaccgccaagggcgaggaggtggaccgcatcgtgggcctggagatcggcgccgacgactacatcccgaagccgttcaacccgcgcgagctgctggcccgcatccgcgccgtgctgcgccgccaggccaacgagctgccgggcgccccgtcgcaggaggaggccgtgatcgccttcggcaagttcaagctgaacctgggcacccgcgagatgttccgcgaggacgagccgatgccgctgacctcgggcgagttcgccgtgctgaaggccctggtgtcgcatccgcgcgagccgctgtcgcgcgacaagctgatgaacctggcccgcggccgcgagtattcggccatggagcgctcgatcgacgtgcagatctcgcgcctgcgccgcatggtggaggaggacccggcccacccgcgctacatccagaccgtgtggggcctgggctacgtgttcgtgccggacggctcgaaggcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagtacacgcaaaccgtcgatttaccagttgggagacgacacagtgactgacgatctgaacaaagtctggccgagcggcctgaccgttgccgaagccgaagaagttcataagcaactcatcctcggcacccgcgtcttcggtggcatggcgctcatcgcgcacttcctcgccgccgctgcgaccccgtggctcggctgataggagaagactgacatgaccaacggcaaaatctggctcgtggtgaaaccgaccgtcggcgttccgctgttcctcagcgctgccgtcatcgcctccgtcgttatccacgctgctgtgctgacgaccaccacctggctgcccgcctactaccaaggctcggctgcggtcgcggccgagtaa BBa_K227010_sequence 1 atgcaggagaactataagatcctggtggtggacgacgacatgcgcctgcgcgccctgctggagcgctacctgaccgagcagggcttccaggtgcgctcggtggccaacgccgagcagatggaccgcctgctgacccgcgagtcgttccatctgatggtgctggacctgatgctgccgggcgaggacggcctgtcgatctgccgccgcctgcgctcgcagtcgaacccgatgccgatcatcatggtgaccgccaagggcgaggaggtggaccgcatcgtgggcctggagatcggcgccgacgactacatcccgaagccgttcaacccgcgcgagctgctggcccgcatccgcgccgtgctgcgccgccaggccaacgagctgccgggcgccccgtcgcaggaggaggccgtgatcgccttcggcaagttcaagctgaacctgggcacccgcgagatgttccgcgaggacgagccgatgccgctgacctcgggcgagttcgccgtgctgaaggccctggtgtcgcatccgcgcgagccgctgtcgcgcgacaagctgatgaacctggcccgcggccgcgagtattcggccatggagcgctcgatcgacgtgcagatctcgcgcctgcgccgcatggtggaggaggacccggcccacccgcgctacatccagaccgtgtggggcctgggctacgtgttcgtgccggacggctcgaaggcctga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z