##gff-version 3
##sequence-region BBa_K227012 1 1375
BBa_K227012	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K227012	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K227012	.	CDS	19	738	.	+	0	ID=BBa_K227010;Name=BBa_K227010
BBa_K227012	.	CDS	19	738	.	+	0	ID=annotation2056947;Name=ompR(sph);Parent=BBa_K227010
BBa_K227012	.	terminator	747	826	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K227012	.	engineered_region	747	826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K227012	.	stem_loop	758	801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K227012	.	terminator	835	875	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K227012	.	stem_loop	842	861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K227012	.	engineered_region	835	875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K227012	.	stop_codon	868	868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K227012	.	polyA_site	862	875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K227012	.	engineered_region	884	1375	.	+	0	ID=BBa_K227008;Name=BBa_K227008
BBa_K227012	.	non_covalent_binding_site	896	913	.	+	0	ID=annotation2057770;Name=C1 OmpR;Parent=BBa_K227008
BBa_K227012	.	engineered_region	1040	1375	.	+	0	ID=annotation2057777;Name=BBa_K227006;Parent=BBa_K227008
BBa_K227012	.	non_covalent_binding_site	917	934	.	+	0	ID=annotation2057771;Name=C2 OmpR;Parent=BBa_K227008
BBa_K227012	.	non_covalent_binding_site	1027	1031	.	+	0	ID=annotation2057775;Name=RBS;Parent=BBa_K227008
BBa_K227012	.	CDS	1211	1375	.	+	0	ID=annotation2057779;Name=pucA;Parent=BBa_K227008
BBa_K227012	.	promoter	958	963	.	+	0	ID=annotation2057773;Name=-35;Parent=BBa_K227008
BBa_K227012	.	non_covalent_binding_site	937	954	.	+	0	ID=annotation2057772;Name=C3 OmpR;Parent=BBa_K227008
BBa_K227012	.	engineered_region	884	991	.	+	0	ID=annotation2057769;Name=BBa_R0082;Parent=BBa_K227008
BBa_K227012	.	CDS	1040	1194	.	+	0	ID=annotation2057776;Name=PucB;Parent=BBa_K227008
BBa_K227012	.	promoter	981	986	.	+	0	ID=annotation2057774;Name=-10;Parent=BBa_K227008
BBa_K227012	.	non_covalent_binding_site	1198	1202	.	+	0	ID=annotation2057778;Name=RBS;Parent=BBa_K227008
>BBa_K227012
aaagaggagaaatactagatgcaggagaactataagatcctggtggtggacgacgacatgcgcctgcgcgccctgctgg
agcgctacctgaccgagcagggcttccaggtgcgctcggtggccaacgccgagcagatggaccgcctgctgacccgcga
gtcgttccatctgatggtgctggacctgatgctgccgggcgaggacggcctgtcgatctgccgccgcctgcgctcgcag
tcgaacccgatgccgatcatcatggtgaccgccaagggcgaggaggtggaccgcatcgtgggcctggagatcggcgccg
acgactacatcccgaagccgttcaacccgcgcgagctgctggcccgcatccgcgccgtgctgcgccgccaggccaacga
gctgccgggcgccccgtcgcaggaggaggccgtgatcgccttcggcaagttcaagctgaacctgggcacccgcgagatg
ttccgcgaggacgagccgatgccgctgacctcgggcgagttcgccgtgctgaaggccctggtgtcgcatccgcgcgagc
cgctgtcgcgcgacaagctgatgaacctggcccgcggccgcgagtattcggccatggagcgctcgatcgacgtgcagat
ctcgcgcctgcgccgcatggtggaggaggacccggcccacccgcgctacatccagaccgtgtggggcctgggctacgtg
ttcgtgccggacggctcgaaggcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatc
atattcgtgttggattattctgcatttttggggagaatggacttactagagtacacgcaaaccgtcgatttaccagttg
ggagacgacacagtgactgacgatctgaacaaagtctggccgagcggcctgaccgttgccgaagccgaagaagttcata
agcaactcatcctcggcacccgcgtcttcggtggcatggcgctcatcgcgcacttcctcgccgccgctgcgaccccgtg
gctcggctgataggagaagactgacatgaccaacggcaaaatctggctcgtggtgaaaccgaccgtcggcgttccgctg
ttcctcagcgctgccgtcatcgcctccgtcgttatccacgctgctgtgctgacgaccaccacctggctgcccgcctact
accaaggctcggctgcggtcgcggccgagtaa