BBa_K227007 1 BBa_K227007 puc promoter 2009-10-11T11:00:00Z 2015-05-08T01:11:32Z PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter. The puc promoter is the primary promoter for the puc operon. The puc operon consists of puc A and B coding regions responsible for the LH II antenna complex essential for light harvesting in Rhodobacter sphaeroides. The puc promoter is ultimately controlled by oxygen tension, and is a very strong promoter under low oxygen concentration. false false _326_ 0 4645 9 It's complicated true - false WashU iGEM 2009 annotation2057660 1 PpsR range2057660 1 482 499 annotation2057661 1 PpsR range2057661 1 507 524 BBa_K227014 1 BBa_K227014 pucpro+pucBA 2009-10-16T11:00:00Z 2015-05-08T01:11:32Z registry part composite puc promotor and pucBA from Rhodobacter sphaeroides. This system is naturally controlled by oxygen tension in R. sphaeroides (high 02=suppressed). pucBA codes for the LHII light harvesting antennae complexes in R. sphaeroides. false false _326_ 0 4645 9 Not in stock false -- false WashU iGEM 2009 component2218777 1 BBa_K227006 component2218773 1 BBa_K227007 annotation2218777 1 BBa_K227006 range2218777 1 660 995 annotation2218773 1 BBa_K227007 range2218773 1 1 651 BBa_K227006 1 BBa_K227006 puc BA coding region of R. sphaeroides 2009-10-11T11:00:00Z 2015-05-08T01:11:32Z PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter. puc BA is the complete LHII transmembrane protein coding region found naturally in Rhodobacter Sphaeroides. LHII complexes form the light-harvesting antennae the funnel energy down to LHI and the reaction center. They are naturally found in the ratio of 3-6.7 LHII per LHI. Data suggests the precence of puc C,D, and E regions also contribute to the formation of a functional protein. false false _326_ 0 4645 9 It's complicated true puc A and puc B together create the antennae complex necessary to harvest varying intensities of light. A common problem in bioreactors is the uneven distribution of light between cells nearest the light source and cells farther away from the light source. One way of combating this problem is modify the antenna size of the organism. By truncating the light-harvesting antenna, cells at the exterior of the bioreactor waste less light through Non-Photochemical Quenching, thus allowing photons to penetrate deeper and reach cells in the interior of the bioreactor. This leads to an increase in photosynthetic efficiency and productivity for the entire culture. false WashU iGEM 2009 annotation2057320 1 RBS range2057320 1 159 163 annotation2057321 1 pucA range2057321 1 172 336 annotation2057319 1 pucB range2057319 1 1 156 BBa_K227006_sequence 1 gtgactgacgatctgaacaaagtctggccgagcggcctgaccgttgccgaagccgaagaagttcataagcaactcatcctcggcacccgcgtcttcggtggcatggcgctcatcgcgcacttcctcgccgccgctgcgaccccgtggctcggctgataggagaagactgacatgaccaacggcaaaatctggctcgtggtgaaaccgaccgtcggcgttccgctgttcctcagcgctgccgtcatcgcctccgtcgttatccacgctgctgtgctgacgaccaccacctggctgcccgcctactaccaaggctcggctgcggtcgcggccgagtaa BBa_K227014_sequence 1 gggcctctttctcggcgtcgaggtgctcgagcccgagtcgcgcgcgcccgatgcccggatggccgcgcgtatcgtgaacgggctgcgcgaggcccgcgtgctgatctcggccaccggaccgcacggccatgtgctgaagatccgcccgccgctcgtcttctcggacgcgaacgcggccttctttctcgagcggttcgagtcggtgctcgcggccctgcgctgagcggggcgtaccgggcagaggccgccaagccatcctgaaatctcgaaaaatccccgcaagatggggtccggaagggccgggcgcaaggcctcggacaccctcgtttttgcagcagcgagaggctgcgggacggccctgtggggccgggacaggcagcgtcaatttcccgcgcgcctgcggcaaaattgtcccttttcaagccgttacgcaggattcccggccgatctggcggccaataagtcgcacccaaaacggccttgtcagccaacactgacattgaatctgtcagcgcaatgtgacacccataatgcgagccggggcggatcagaaatcgccgacaaggtgatccaggtctctccggtctcgtcgaagcccgcgtgcaggccctacacgcaaaccgtcgatttaccagttgggagacgacacagtactagaggtgactgacgatctgaacaaagtctggccgagcggcctgaccgttgccgaagccgaagaagttcataagcaactcatcctcggcacccgcgtcttcggtggcatggcgctcatcgcgcacttcctcgccgccgctgcgaccccgtggctcggctgataggagaagactgacatgaccaacggcaaaatctggctcgtggtgaaaccgaccgtcggcgttccgctgttcctcagcgctgccgtcatcgcctccgtcgttatccacgctgctgtgctgacgaccaccacctggctgcccgcctactaccaaggctcggctgcggtcgcggccgagtaa BBa_K227007_sequence 1 gggcctctttctcggcgtcgaggtgctcgagcccgagtcgcgcgcgcccgatgcccggatggccgcgcgtatcgtgaacgggctgcgcgaggcccgcgtgctgatctcggccaccggaccgcacggccatgtgctgaagatccgcccgccgctcgtcttctcggacgcgaacgcggccttctttctcgagcggttcgagtcggtgctcgcggccctgcgctgagcggggcgtaccgggcagaggccgccaagccatcctgaaatctcgaaaaatccccgcaagatggggtccggaagggccgggcgcaaggcctcggacaccctcgtttttgcagcagcgagaggctgcgggacggccctgtggggccgggacaggcagcgtcaatttcccgcgcgcctgcggcaaaattgtcccttttcaagccgttacgcaggattcccggccgatctggcggccaataagtcgcacccaaaacggccttgtcagccaacactgacattgaatctgtcagcgcaatgtgacacccataatgcgagccggggcggatcagaaatcgccgacaaggtgatccaggtctctccggtctcgtcgaagcccgcgtgcaggccctacacgcaaaccgtcgatttaccagttgggagacgacacag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z