BBa_K227007
1
BBa_K227007
puc promoter
2009-10-11T11:00:00Z
2015-05-08T01:11:32Z
PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter.
The puc promoter is the primary promoter for the puc operon. The puc operon consists of puc A and B coding regions responsible for the LH II antenna complex essential for light harvesting in Rhodobacter sphaeroides. The puc promoter is ultimately controlled by oxygen tension, and is a very strong promoter under low oxygen concentration.
false
false
_326_
0
4645
9
It's complicated
true
-
false
WashU iGEM 2009
annotation2057660
1
PpsR
range2057660
1
482
499
annotation2057661
1
PpsR
range2057661
1
507
524
BBa_K227014
1
BBa_K227014
pucpro+pucBA
2009-10-16T11:00:00Z
2015-05-08T01:11:32Z
registry part composite
puc promotor and pucBA from Rhodobacter sphaeroides. This system is naturally controlled by oxygen tension in R. sphaeroides (high 02=suppressed). pucBA codes for the LHII light harvesting antennae complexes in R. sphaeroides.
false
false
_326_
0
4645
9
Not in stock
false
--
false
WashU iGEM 2009
component2218777
1
BBa_K227006
component2218773
1
BBa_K227007
annotation2218777
1
BBa_K227006
range2218777
1
660
995
annotation2218773
1
BBa_K227007
range2218773
1
1
651
BBa_K227006
1
BBa_K227006
puc BA coding region of R. sphaeroides
2009-10-11T11:00:00Z
2015-05-08T01:11:32Z
PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter.
puc BA is the complete LHII transmembrane protein coding region found naturally in Rhodobacter Sphaeroides. LHII complexes form the light-harvesting antennae the funnel energy down to LHI and the reaction center. They are naturally found in the ratio of 3-6.7 LHII per LHI. Data suggests the precence of puc C,D, and E regions also contribute to the formation of a functional protein.
false
false
_326_
0
4645
9
It's complicated
true
puc A and puc B together create the antennae complex necessary to harvest varying intensities of light. A common problem in bioreactors is the uneven distribution of light between cells nearest the light source and cells farther away from the light source. One way of combating this problem is modify the antenna size of the organism. By truncating the light-harvesting antenna, cells at the exterior of the bioreactor waste less light through Non-Photochemical Quenching, thus allowing photons to penetrate deeper and reach cells in the interior of the bioreactor. This leads to an increase in photosynthetic efficiency and productivity for the entire culture.
false
WashU iGEM 2009
annotation2057320
1
RBS
range2057320
1
159
163
annotation2057321
1
pucA
range2057321
1
172
336
annotation2057319
1
pucB
range2057319
1
1
156
BBa_K227006_sequence
1
gtgactgacgatctgaacaaagtctggccgagcggcctgaccgttgccgaagccgaagaagttcataagcaactcatcctcggcacccgcgtcttcggtggcatggcgctcatcgcgcacttcctcgccgccgctgcgaccccgtggctcggctgataggagaagactgacatgaccaacggcaaaatctggctcgtggtgaaaccgaccgtcggcgttccgctgttcctcagcgctgccgtcatcgcctccgtcgttatccacgctgctgtgctgacgaccaccacctggctgcccgcctactaccaaggctcggctgcggtcgcggccgagtaa
BBa_K227014_sequence
1
gggcctctttctcggcgtcgaggtgctcgagcccgagtcgcgcgcgcccgatgcccggatggccgcgcgtatcgtgaacgggctgcgcgaggcccgcgtgctgatctcggccaccggaccgcacggccatgtgctgaagatccgcccgccgctcgtcttctcggacgcgaacgcggccttctttctcgagcggttcgagtcggtgctcgcggccctgcgctgagcggggcgtaccgggcagaggccgccaagccatcctgaaatctcgaaaaatccccgcaagatggggtccggaagggccgggcgcaaggcctcggacaccctcgtttttgcagcagcgagaggctgcgggacggccctgtggggccgggacaggcagcgtcaatttcccgcgcgcctgcggcaaaattgtcccttttcaagccgttacgcaggattcccggccgatctggcggccaataagtcgcacccaaaacggccttgtcagccaacactgacattgaatctgtcagcgcaatgtgacacccataatgcgagccggggcggatcagaaatcgccgacaaggtgatccaggtctctccggtctcgtcgaagcccgcgtgcaggccctacacgcaaaccgtcgatttaccagttgggagacgacacagtactagaggtgactgacgatctgaacaaagtctggccgagcggcctgaccgttgccgaagccgaagaagttcataagcaactcatcctcggcacccgcgtcttcggtggcatggcgctcatcgcgcacttcctcgccgccgctgcgaccccgtggctcggctgataggagaagactgacatgaccaacggcaaaatctggctcgtggtgaaaccgaccgtcggcgttccgctgttcctcagcgctgccgtcatcgcctccgtcgttatccacgctgctgtgctgacgaccaccacctggctgcccgcctactaccaaggctcggctgcggtcgcggccgagtaa
BBa_K227007_sequence
1
gggcctctttctcggcgtcgaggtgctcgagcccgagtcgcgcgcgcccgatgcccggatggccgcgcgtatcgtgaacgggctgcgcgaggcccgcgtgctgatctcggccaccggaccgcacggccatgtgctgaagatccgcccgccgctcgtcttctcggacgcgaacgcggccttctttctcgagcggttcgagtcggtgctcgcggccctgcgctgagcggggcgtaccgggcagaggccgccaagccatcctgaaatctcgaaaaatccccgcaagatggggtccggaagggccgggcgcaaggcctcggacaccctcgtttttgcagcagcgagaggctgcgggacggccctgtggggccgggacaggcagcgtcaatttcccgcgcgcctgcggcaaaattgtcccttttcaagccgttacgcaggattcccggccgatctggcggccaataagtcgcacccaaaacggccttgtcagccaacactgacattgaatctgtcagcgcaatgtgacacccataatgcgagccggggcggatcagaaatcgccgacaaggtgatccaggtctctccggtctcgtcgaagcccgcgtgcaggccctacacgcaaaccgtcgatttaccagttgggagacgacacag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z