LOCUS       BBa_K227015             1875 bp    DNA     linear       22-Dec-2024
DEFINITION  RBS34+OmpR(sph)+Term+ompC+GFP
ACCESSION   BBa_K227015
VERSION     BBa_K227015.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=ompR(sph)
     CDS             19..738
                     /label=BBa_K227010
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     misc_feature    896..913
                     /label=C1 OmpR
     misc_feature    917..934
                     /label=C2 OmpR
     misc_feature    937..954
                     /label=C3 OmpR
     promoter        958..963
                     /label=-35
     promoter        981..986
                     /label=-10
     promoter        884..991
                     /label=BBa_R0082
     RBS             1000..1012
                     /label=RBS-3\Weak
     RBS             1006..1009
                     /label=RBS
     misc_feature    1000..1012
                     /label=BBa_B0032
     RBS             1000..1012
                     /label=BBa_B0032
     CDS             1019..1738
                     /label=GFP protein
     CDS             1019..1738
                     /label=BBa_E0040
     stem_loop       1758..1801
                     /label=stem_loop
     misc_feature    1747..1826
                     /label=BBa_B0010
     terminator      1747..1826
                     /label=BBa_B0010
     stem_loop       1842..1861
                     /label=T7 TE
     misc_feature    1868..1868
                     /label=stop
     polyA_site      1862..1875
                     /label=polya
     misc_feature    1835..1875
                     /label=BBa_B0012
     terminator      1835..1875
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gcaggagaac tataagatcc tggtggtgga cgacgacatg
       61 cgcctgcgcg ccctgctgga gcgctacctg accgagcagg gcttccaggt gcgctcggtg
      121 gccaacgccg agcagatgga ccgcctgctg acccgcgagt cgttccatct gatggtgctg
      181 gacctgatgc tgccgggcga ggacggcctg tcgatctgcc gccgcctgcg ctcgcagtcg
      241 aacccgatgc cgatcatcat ggtgaccgcc aagggcgagg aggtggaccg catcgtgggc
      301 ctggagatcg gcgccgacga ctacatcccg aagccgttca acccgcgcga gctgctggcc
      361 cgcatccgcg ccgtgctgcg ccgccaggcc aacgagctgc cgggcgcccc gtcgcaggag
      421 gaggccgtga tcgccttcgg caagttcaag ctgaacctgg gcacccgcga gatgttccgc
      481 gaggacgagc cgatgccgct gacctcgggc gagttcgccg tgctgaaggc cctggtgtcg
      541 catccgcgcg agccgctgtc gcgcgacaag ctgatgaacc tggcccgcgg ccgcgagtat
      601 tcggccatgg agcgctcgat cgacgtgcag atctcgcgcc tgcgccgcat ggtggaggag
      661 gacccggccc acccgcgcta catccagacc gtgtggggcc tgggctacgt gttcgtgccg
      721 gacggctcga aggcctgata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtcccttg catttacatt
      901 ttgaaacatc tatagcgata aatgaaacat cttaaaagtt ttagtatcat attcgtgttg
      961 gattattctg catttttggg gagaatggac ttactagagt cacacaggaa agtactagat
     1021 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1081 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     1141 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     1201 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     1261 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     1321 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     1381 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     1441 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     1501 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     1561 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     1621 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     1681 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata
     1741 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1801 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1861 ctttctgcgt ttata
//