##gff-version 3
##sequence-region BBa_K227015 1 1875
BBa_K227015	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K227015	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K227015	.	CDS	1019	1738	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K227015	.	CDS	1019	1738	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K227015	.	terminator	747	826	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K227015	.	engineered_region	747	826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K227015	.	stem_loop	758	801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K227015	.	terminator	835	875	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K227015	.	stem_loop	842	861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K227015	.	engineered_region	835	875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K227015	.	stop_codon	868	868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K227015	.	polyA_site	862	875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K227015	.	ribosome_entry_site	1000	1012	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K227015	.	ribosome_entry_site	1000	1012	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K227015	.	ribosome_entry_site	1006	1009	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K227015	.	engineered_region	1000	1012	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K227015	.	terminator	1835	1875	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K227015	.	stem_loop	1842	1861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K227015	.	engineered_region	1835	1875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K227015	.	stop_codon	1868	1868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K227015	.	polyA_site	1862	1875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K227015	.	promoter	884	991	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K227015	.	promoter	958	963	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K227015	.	non_covalent_binding_site	937	954	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K227015	.	promoter	981	986	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K227015	.	non_covalent_binding_site	917	934	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K227015	.	non_covalent_binding_site	896	913	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K227015	.	CDS	19	738	.	+	0	ID=BBa_K227010;Name=BBa_K227010
BBa_K227015	.	CDS	19	738	.	+	0	ID=annotation2056947;Name=ompR(sph);Parent=BBa_K227010
BBa_K227015	.	terminator	1747	1826	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K227015	.	engineered_region	1747	1826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K227015	.	stem_loop	1758	1801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K227015
aaagaggagaaatactagatgcaggagaactataagatcctggtggtggacgacgacatgcgcctgcgcgccctgctgg
agcgctacctgaccgagcagggcttccaggtgcgctcggtggccaacgccgagcagatggaccgcctgctgacccgcga
gtcgttccatctgatggtgctggacctgatgctgccgggcgaggacggcctgtcgatctgccgccgcctgcgctcgcag
tcgaacccgatgccgatcatcatggtgaccgccaagggcgaggaggtggaccgcatcgtgggcctggagatcggcgccg
acgactacatcccgaagccgttcaacccgcgcgagctgctggcccgcatccgcgccgtgctgcgccgccaggccaacga
gctgccgggcgccccgtcgcaggaggaggccgtgatcgccttcggcaagttcaagctgaacctgggcacccgcgagatg
ttccgcgaggacgagccgatgccgctgacctcgggcgagttcgccgtgctgaaggccctggtgtcgcatccgcgcgagc
cgctgtcgcgcgacaagctgatgaacctggcccgcggccgcgagtattcggccatggagcgctcgatcgacgtgcagat
ctcgcgcctgcgccgcatggtggaggaggacccggcccacccgcgctacatccagaccgtgtggggcctgggctacgtg
ttcgtgccggacggctcgaaggcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatc
atattcgtgttggattattctgcatttttggggagaatggacttactagagtcacacaggaaagtactagatgcgtaaa
ggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtca
gtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcc
atggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgac
tttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacac
gtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaa
cattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatc
aaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaa
ttggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaa
gagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata