BBa_K227015
type: ComponentDefinition
persistent identity: BBa K227015
display id: BBa_K227015
version: 1
was generated by: iGEM to SBOL conversion
description: RBS34+OmpR(sph)+Term+ompC+GFP
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: composite part
owned by: james
owned by: myers
mutable description: RBS34 and OmpR (sphaeroides codon-optimized), along with ompC controlled expression of GFP. combined with Cph8 in Rhodobacter sphaeroides, this system will produce GFP in response to varying light intensity.
discontinued: false
dominant: false
experience: experience/None
group u list: _326_
m user id: 0
owner id: 4645
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: OmpR (sph) is codon-optimized for use in R. Sphaeroides.
star: false
creator: WashU iGEM 2009
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_B0032
component: BBa_R0082
component: BBa_E0040
component: BBa_K227010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K227010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0082
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K227015_sequence/1

BBa_B0010
type: Component
persistent identity: component2057701
display id: component2057701
version: 1
top level: BBa_K227015
definition: BBa_B0010
access: public

BBa_B0012
type: Component
persistent identity: component2057720
display id: component2057720
version: 1
top level: BBa_K227015
definition: BBa_B0012
access: public

BBa_B0032
type: Component
persistent identity: component2057714
display id: component2057714
version: 1
top level: BBa_K227015
definition: BBa_B0032
access: public

BBa_R0082
type: Component
persistent identity: component2057712
display id: component2057712
version: 1
top level: BBa_K227015
definition: OmpR
access: public

BBa_B0010
type: Component
persistent identity: component2057718
display id: component2057718
version: 1
top level: BBa_K227015
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component2057717
display id: component2057717
version: 1
top level: BBa_K227015
definition: GFP
access: public

BBa_K227010
type: Component
persistent identity: component2057700
display id: component2057700
version: 1
top level: BBa_K227015
definition: BBa_K227010
access: public

BBa_B0034
type: Component
persistent identity: component2057698
display id: component2057698
version: 1
top level: BBa_K227015
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2057703
display id: component2057703
version: 1
top level: BBa_K227015
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2057698
display id: annotation2057698
version: 1
top level: BBa_K227015
location: https://synbiohub.org/public/igem/BBa_K227015/annotation2057698/range2057698/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K227015/annotation2057698/range2057698/1
type: Range
persistent identity: range2057698
display id: range2057698
version: 1
top level: BBa_K227015
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K227010
type: SequenceAnnotation
persistent identity: annotation2057700
display id: annotation2057700
version: 1
top level: BBa_K227015
location: https://synbiohub.org/public/igem/BBa_K227015/annotation2057700/range2057700/1
component: BBa_K227010

https://synbiohub.org/public/igem/BBa_K227015/annotation2057700/range2057700/1
type: Range
persistent identity: range2057700
display id: range2057700
version: 1
top level: BBa_K227015
direction: direction/reverse
start: 19
end: 738
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2057701
display id: annotation2057701
version: 1
top level: BBa_K227015
location: https://synbiohub.org/public/igem/BBa_K227015/annotation2057701/range2057701/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K227015/annotation2057701/range2057701/1
type: Range
persistent identity: range2057701
display id: range2057701
version: 1
top level: BBa_K227015
direction: direction/forward
start: 747
end: 826
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2057703
display id: annotation2057703
version: 1
top level: BBa_K227015
location: https://synbiohub.org/public/igem/BBa_K227015/annotation2057703/range2057703/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K227015/annotation2057703/range2057703/1
type: Range
persistent identity: range2057703
display id: range2057703
version: 1
top level: BBa_K227015
direction: direction/forward
start: 835
end: 875
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2057712
display id: annotation2057712
version: 1
top level: BBa_K227015
location: https://synbiohub.org/public/igem/BBa_K227015/annotation2057712/range2057712/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K227015/annotation2057712/range2057712/1
type: Range
persistent identity: range2057712
display id: range2057712
version: 1
top level: BBa_K227015
direction: direction/reverse
start: 884
end: 991
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2057714
display id: annotation2057714
version: 1
top level: BBa_K227015
location: https://synbiohub.org/public/igem/BBa_K227015/annotation2057714/range2057714/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K227015/annotation2057714/range2057714/1
type: Range
persistent identity: range2057714
display id: range2057714
version: 1
top level: BBa_K227015
direction: direction/forward
start: 1000
end: 1012
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2057717
display id: annotation2057717
version: 1
top level: BBa_K227015
location: https://synbiohub.org/public/igem/BBa_K227015/annotation2057717/range2057717/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K227015/annotation2057717/range2057717/1
type: Range
persistent identity: range2057717
display id: range2057717
version: 1
top level: BBa_K227015
direction: direction/reverse
start: 1019
end: 1738
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2057718
display id: annotation2057718
version: 1
top level: BBa_K227015
location: https://synbiohub.org/public/igem/BBa_K227015/annotation2057718/range2057718/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K227015/annotation2057718/range2057718/1
type: Range
persistent identity: range2057718
display id: range2057718
version: 1
top level: BBa_K227015
direction: direction/forward
start: 1747
end: 1826
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2057720
display id: annotation2057720
version: 1
top level: BBa_K227015
location: https://synbiohub.org/public/igem/BBa_K227015/annotation2057720/range2057720/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K227015/annotation2057720/range2057720/1
type: Range
persistent identity: range2057720
display id: range2057720
version: 1
top level: BBa_K227015
direction: direction/forward
start: 1835
end: 1875
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K227010
type: ComponentDefinition
persistent identity: BBa K227010
display id: BBa_K227010
version: 1
was generated by: iGEM to SBOL conversion
description: OmpR (sphaeroides)
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: sequence modified from part BBa_(((()))))
owned by: james
owned by: myers
mutable description: OmpR promotor from E.coli, with DNA codon usage optimized for use in Rhodobacter sphaeroides.
discontinued: false
dominant: false
experience: experience/None
group u list: _326_
m user id: 0
owner id: 4645
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: codon usage optimized for Rhodobacter sphaeroides using GeneDesigner.
star: false
creator: WashU iGEM 2009
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ompR(sph)
sequence: https://synbiohub.org/public/igem/BBa_K227010_sequence/1

ompR(sph)
type: SequenceAnnotation
persistent identity: annotation2056947
display id: annotation2056947
version: 1
top level: BBa_K227010
location: https://synbiohub.org/public/igem/BBa_K227010/annotation2056947/range2056947/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K227010/annotation2056947/range2056947/1
type: Range
persistent identity: range2056947
display id: range2056947
version: 1
top level: BBa_K227010
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_K227015_sequence/1
type: Sequence
persistent identity: BBa K227015 sequence
display id: BBa_K227015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcaggagaactataagatcctggtggtggacgacgacatgcgcctgcgcgccctgctggagcgctacctgaccgagcagggcttccaggtgcgctcggtggccaacgccgagcagatggaccgcctgctgacccgcgagtcgttccatctgatggtgctggacctgatgctgccgggcgaggacggcctgtcgatctgccgccgcctgcgctcgcagtcgaacccgatgccgatcatcatggtgaccgccaagggcgaggaggtggaccgcatcgtgggcctggagatcggcgccgacgactacatcccgaagccgttcaacccgcgcgagctgctggcccgcatccgcgccgtgctgcgccgccaggccaacgagctgccgggcgccccgtcgcaggaggaggccgtgatcgccttcggcaagttcaagctgaacctgggcacccgcgagatgttccgcgaggacgagccgatgccgctgacctcgggcgagttcgccgtgctgaaggccctggtgtcgcatccgcgcgagccgctgtcgcgcgacaagctgatgaacctggcccgcggccgcgagtattcggccatggagcgctcgatcgacgtgcagatctcgcgcctgcgccgcatggtggaggaggacccggcccacccgcgctacatccagaccgtgtggggcctgggctacgtgttcgtgccggacggctcgaaggcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K227010_sequence/1
type: Sequence
persistent identity: BBa K227010 sequence
display id: BBa_K227010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaggagaactataagatcctggtggtggacgacgacatgcgcctgcgcgccctgctggagcgctacctgaccgagcagggcttccaggtgcgctcggtggccaacgccgagcagatggaccgcctgctgacccgcgagtcgttccatctgatggtgctggacctgatgctgccgggcgaggacggcctgtcgatctgccgccgcctgcgctcgcagtcgaacccgatgccgatcatcatggtgaccgccaagggcgaggaggtggaccgcatcgtgggcctggagatcggcgccgacgactacatcccgaagccgttcaacccgcgcgagctgctggcccgcatccgcgccgtgctgcgccgccaggccaacgagctgccgggcgccccgtcgcaggaggaggccgtgatcgccttcggcaagttcaagctgaacctgggcacccgcgagatgttccgcgaggacgagccgatgccgctgacctcgggcgagttcgccgtgctgaaggccctggtgtcgcatccgcgcgagccgctgtcgcgcgacaagctgatgaacctggcccgcggccgcgagtattcggccatggagcgctcgatcgacgtgcagatctcgcgcctgcgccgcatggtggaggaggacccggcccacccgcgctacatccagaccgtgtggggcctgggctacgtgttcgtgccggacggctcgaaggcctga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z