LOCUS       BBa_K228003             2938 bp    DNA     linear       05-Nov-2024
DEFINITION  Bistable part
ACCESSION   BBa_K228003
VERSION     BBa_K228003.1  
FEATURES             Location/Qualifiers
     CDS             1..681
                     /label=mRFP
     CDS             2219..2938
                     /label=eGFP
     CDS             1561..2196
                     /label=CI434 repressor
     RBS             2203..2211
                     /label=rbs
     CDS             707..1420
                     /label=lamda CI repressor
     RBS             688..700
                     /label=rbs
     RBS             1426..1432
                     /label=rbs
     promoter        1491..1539
                     /label=CI regulated promoter
     RBS             1546..1554
                     /label=rbs
     misc_feature    1439..1454
                     /label=CI434 OR1
     promoter        1439..1490
                     /label=CI434 regulated promoter
ORIGIN
        1 ttattaagca ccggtggagt gacgaccttc agcacgttcg tactgttcaa cgatggtgta
       61 gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg
      121 aaccggtttt ttagccatgt aggtggtttt aacttcagcg tcgtagtgac caccgtcttt
      181 cagtttcaga cgcattttga tttcaccttt cagagcaccg tcttccgggt acatacgttc
      241 ggtggaagct tcccaaccca tggttttttt ctgcataacc ggaccgtcgg acgggaagtt
      301 ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt
      361 aacggtaaca acaccaccgt cttcgaagtt cataacacgt tcccatttga aaccttccgg
      421 gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc
      481 gtactggaac tgcggggaca ggatgtccca agcgaacggc agcggaccac ctttggtaac
      541 tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac cttcgatttc
      601 gaactcgtga ccgttaacgg aaccttccat acgaactttg aaacgcatga actctttgat
      661 aacgtcttcg gaggaagcca tctagtattt ctcctctttc actagatcag ccaaacgtct
      721 cttcaggcca ctgactagcg ataactttcc ccacaacgga acaactctca ttgcatggga
      781 tcattgggta ctgtgggttt agtggttgta aaaacacctg accgctatcc ctgatcagtt
      841 tcttgaaggt aaactcatca cccccaagtc tggctatgca gaaatcacct ggctcaacag
      901 cctgctcagg gtcaacgaga attaacattc cgtcaggaaa gcttggcttg gagcctgttg
      961 gtgcggtcat ggaattacct tcaacctcaa gccagaatgc agaatcactg gcttttttgg
     1021 ttgtgcttac ccatctctcc gcatcacctt tggtaaaggt tctaagctca ggtgagaaca
     1081 tccctgcctg aacatgagaa aaaacagggt actcatactc acttctaagt gacggctgca
     1141 tactaaccgc ttcatacatc tcgtagattt ctctggcgat tgaagggcta aattcttcaa
     1201 cgctaacttt gagaattttt gcaagcaatg cggcgttata agcatttaat gcattgatgc
     1261 cattaaataa agcaccaacg cctgactgcc ccatccccat cttgtctgcg acagattcct
     1321 gggataagcc aagttcattt ttcttttttt cataaattgc tttaaggcga cgtgcgtcct
     1381 caagctgctc ttgtgttaat ggtttctttt ttgtgctcat ttttttcctc ctactagaaa
     1441 caaactttct tgtacatacg ttaaatctaa caaactttct tgtaaatatc taacaccgtg
     1501 cgtgttgact attttacctc tggcggtgat aatggttgca ctagatacta aggaggttgt
     1561 atgagtattt cttccagggt aaaaagcaaa agaatccagc ttggacttaa ccaggctgaa
     1621 cttgctcaaa aggtggggac tacccagcag tctatagagc agctcgaaaa cggtaaaact
     1681 aagcgaccac gctttttacc agaacttgcg tcagctcttg gcgtaagtgt tgactggctg
     1741 ctcaatggca cctctgattc gaatgttaga tttgttgggc acgttgagcc caaagggaaa
     1801 tatccattga ttagcatggt tagagctggt tcgtggtgtg aagcttgtga accctacgat
     1861 atcaaggaca ttgatgaatg gtatgacagt gacgttaact tattaggcaa tggattctgg
     1921 ctgaaggttg aaggtgattc catgacctca cctgtaggtc aaagcatccc tgaaggtcat
     1981 atggtgttag tagatactgg acgggagcca gtgaatggaa gccttgttgt agccaaactg
     2041 actgacgcga acgaagcaac attcaagaaa ctggtcatag atggcggtca gaagtacctg
     2101 aaaggcctga atccttcatg gcctatgact cctatcaacg gaaactgcaa gattatcggt
     2161 gttgtcgtgg aagcgagggt aaaattcgta taataaacta gaagaggaga aattaagcat
     2221 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2281 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     2341 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2401 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     2461 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2521 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2581 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2641 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2701 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2761 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2821 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2881 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaa
//