It generates AraC regulatory protein which binds to and repress the pBad promoter(such as BBa_I13453). L-arabinose, which acts as inducer, could efficiently prevent the protein binding to pBad.
Constitutive AraC regulatory protein generator
This part constitutively generates AraC protein because of the upstream promoter P(cat)(BBa_I14033).
Constitutive Promoter, Medium Transcription
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
The reverse sequence of AraC generator part (BBa_K228005) but of the same efficiency. It generates AraC regulatory protein which binds to and repress the pBad promoter(such as BBa_I13453). L-arabinose, which acts as inducer, could efficiently prevent the protein binding to pBad.
Double terminator consisting of BBa_B0010 and BBa_B0012
araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1