LOCUS       BBa_K228009             1279 bp    DNA     linear       25-Dec-2024
DEFINITION  AraC protein(reversed sequence) and Pbad promoter
ACCESSION   BBa_K228009
VERSION     BBa_K228009.1  
FEATURES             Location/Qualifiers
     promoter        1150..1279
                     /label=BBa_I13453
     misc_feature    1..38
                     /label=P(Cat)
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=BBa_I14033
     misc_feature    47..58
                     /label=BBa_B0034
     misc_feature    51..54
                     /label=conserved
     misc_feature    65..979
                     /label=BBa_C0080
     CDS             65..940
                     /label=araC
     misc_feature    941..973
                     /label=LVA
     misc_feature    980..1004
                     /label=barcode
     misc_feature    1013..1092
                     /label=BBa_B0010
     stem_loop       1024..1067
                     /label=stem_loop
     misc_feature    1101..1141
                     /label=BBa_B0012
     stem_loop       1108..1127
                     /label=T7 TE
     polyA_site      1128..1141
                     /label=polya
     misc_feature    1134..1134
                     /label=stop
     misc_feature    47..1141
                     /label=BBa_K228006
     misc_feature    1150..1279
                     /label=BBa_I13453
     misc_feature    1..1141
                     /label=BBa_K228008
     promoter        1150..1279
                     /label=BBa_I13453
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca
      241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata
      301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct
      361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc
      421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac
      481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
      541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa
      601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
      661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
      721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
      781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
      841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg
      901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
      961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac
     1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta
     1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc
     1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     1261 accgtttttt tgggctagc
//