LOCUS       BBa_K228013             2038 bp    DNA     linear       22-Nov-2024
DEFINITION  (pSal PO) OR Gate - GFP
ACCESSION   BBa_K228013
VERSION     BBa_K228013.1  
FEATURES             Location/Qualifiers
     CDS             1..903
                     /label=NahR
     promoter        904..993
                     /label=Pr promoter
     promoter        982..1050
                     /label=pSal
     promoter        1..1052
                     /label=BBa_K228004
     promoter        1061..1152
                     /label=PO
     promoter        1061..1152
                     /label=BBa_I746361
     RBS             1161..1175
                     /label=RBS-1\Strong
     RBS             1168..1172
                     /label=RBS
     misc_feature    1161..1175
                     /label=BBa_B0030
     RBS             1161..1175
                     /label=BBa_B0030
     CDS             1182..1901
                     /label=GFP protein
     CDS             1182..1901
                     /label=BBa_E0040
     stem_loop       1921..1964
                     /label=stem_loop
     misc_feature    1910..1989
                     /label=BBa_B0010
     terminator      1910..1989
                     /label=BBa_B0010
     stem_loop       2005..2024
                     /label=T7 TE
     misc_feature    2031..2031
                     /label=stop
     polyA_site      2025..2038
                     /label=polya
     misc_feature    1998..2038
                     /label=BBa_B0012
     terminator      1998..2038
                     /label=BBa_B0012
ORIGIN
        1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg
       61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa
      121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag
      181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat
      241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc
      301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt
      361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa
      421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa
      481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg
      541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc
      601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt
      661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg
      721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt
      781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg
      841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc
      901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga
      961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca
     1021 agtcatcaat aaagccatca cgagtaccat agtactagag cgcgccccgc gattcgctaa
     1081 ggtgctgttg tgtcagtgat aagccatccg ggactgatgg cggaggatgc gcatcgtcgg
     1141 gaaactgatg cctactagag attaaagagg agaaatacta gatgcgtaaa ggagaagaac
     1201 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     1261 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     1321 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
     1381 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
     1441 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     1501 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     1561 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     1621 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     1681 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     1741 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     1801 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     1861 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
     1921 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1981 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//