##gff-version 3
##sequence-region BBa_K228039 1 5313
BBa_K228039	.	ribosome_entry_site	3937	3948	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K228039	.	conserved_region	3941	3944	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228039	.	engineered_region	3891	5313	.	+	0	ID=BBa_K228012;Name=BBa_K228012
BBa_K228039	.	mature_transcript_region	5178	5313	.	+	0	ID=BBa_K228001;Name=BBa_K228001;Parent=BBa_K228012
BBa_K228039	.	promoter	3891	5169	.	+	0	ID=BBa_K228009;Name=BBa_K228009;Parent=BBa_K228012
BBa_K228039	.	promoter	5040	5169	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228039	.	promoter	5040	5169	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228039	.	engineered_region	3891	5031	.	+	0	ID=BBa_K228008;Name=BBa_K228008;Parent=BBa_K228009
BBa_K228039	.	operator	3891	3928	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K228039	.	engineered_region	3937	3948	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K228039	.	promoter	3917	3922	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K228039	.	promoter	3894	3899	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K228039	.	polyA_site	5018	5031	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K228039	.	engineered_region	4991	5031	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K228039	.	tag	4831	4863	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K228039	.	stem_loop	4998	5017	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K228039	.	CDS	3955	4830	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K228039	.	engineered_region	3891	3928	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K228039	.	engineered_region	3955	4869	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K228039	.	engineered_region	3937	5031	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K228039	.	stop_codon	5024	5024	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K228039	.	stem_loop	4914	4957	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K228039	.	conserved_region	3941	3944	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K228039	.	engineered_region	5040	5169	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K228039	.	engineered_region	4870	4894	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K228039	.	engineered_region	4903	4982	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K228039	.	engineered_region	1	3882	.	+	0	ID=BBa_K228219;Name=BBa_K228219
BBa_K228039	.	engineered_region	1053	3882	.	+	0	ID=BBa_K228201;Name=BBa_K228201;Parent=BBa_K228219
BBa_K228039	.	ribosome_entry_site	1053	1066	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K228201
BBa_K228039	.	conserved_region	1059	1062	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K228039	.	terminator	3754	3833	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228201
BBa_K228039	.	engineered_region	3754	3833	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228039	.	stem_loop	3765	3808	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228039	.	terminator	3842	3882	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228201
BBa_K228039	.	engineered_region	3842	3882	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228039	.	polyA_site	3869	3882	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228039	.	stem_loop	3849	3868	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228039	.	stop_codon	3875	3875	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228039	.	CDS	1073	3745	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228201
BBa_K228039	.	CDS	1073	3745	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228039	.	engineered_region	1	1044	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_K228219
BBa_K228039	.	promoter	990	1044	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_K228039	.	engineered_region	990	1044	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K228039	.	promoter	1031	1036	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K228039	.	start_codon	1042	1042	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K228039	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K228039	.	promoter	1009	1014	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K228039	.	engineered_region	46	981	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_K228039	.	terminator	853	932	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K228039	.	engineered_region	853	932	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228039	.	stem_loop	864	907	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228039	.	terminator	941	981	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K228039	.	engineered_region	941	981	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228039	.	polyA_site	968	981	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228039	.	stem_loop	948	967	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228039	.	stop_codon	974	974	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228039	.	CDS	64	819	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K228039	.	point_mutation	555	555	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K228039	.	engineered_tag	820	844	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K228039	.	engineered_region	64	819	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K228039	.	point_mutation	237	237	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K228039	.	engineered_region	64	65	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K228039	.	CDS	64	813	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K228039	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K228039	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228039	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_K228039	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K228039	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K228039	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K228039	.	terminator	4991	5031	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228039	.	engineered_region	4991	5031	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228039	.	polyA_site	5018	5031	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228039	.	stem_loop	4998	5017	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228039	.	stop_codon	5024	5024	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228039	.	terminator	4903	4982	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228039	.	engineered_region	4903	4982	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228039	.	stem_loop	4914	4957	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K228039
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagtcacacaggaaacctactagatgaccatgattaccgtgcactagaataccatta
acattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcg
tttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgt
caacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcac
gcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaa
gccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggct
gtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttca
agaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctga
catgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgc
tgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctg
agactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgtt
ccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctg
gcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgatta
acattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattg
tccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggct
ctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagt
tcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgt
ttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatc
ggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgca
tcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagca
agattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcc
cttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcg
cggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagc
agacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaag
ctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgc
tggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaa
gggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtg
gtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactg
gagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcc
tattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgag
attgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactg
tagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgc
tgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgac
cagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtg
acatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttca
ccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagt
gatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggcta
catcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaa
atagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcc
tggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacg
gcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagc
ctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagc
agatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaat
gcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagta
ggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcct
ggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatgg
tgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgt
taaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttc
tcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcac
ggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactct
ctactgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccgg
tctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaag
ctaaggattttttttaagct