LOCUS BBa_K228040 5312 bp DNA linear 22-Nov-2024 DEFINITION AND Gate LuxR-rbs(B0032)-T7ptag-AraC-SupD ACCESSION BBa_K228040 VERSION BBa_K228040.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 RBS 1053..1065 /label=RBS-3\Weak RBS 1059..1062 /label=RBS misc_feature 1053..1065 /label=BBa_B0032 RBS 1053..1065 /label=BBa_B0032 CDS 1072..3744 /label=cds CDS 1072..3744 /label=BBa_K228000 stem_loop 3764..3807 /label=stem_loop misc_feature 3753..3832 /label=BBa_B0010 terminator 3753..3832 /label=BBa_B0010 stem_loop 3848..3867 /label=T7 TE misc_feature 3874..3874 /label=stop polyA_site 3868..3881 /label=polya misc_feature 3841..3881 /label=BBa_B0012 terminator 3841..3881 /label=BBa_B0012 misc_feature 1053..3881 /label=BBa_K228202 misc_feature 1..3881 /label=BBa_K228220 misc_feature 3940..3943 /label=conserved RBS 3936..3947 /label=BBa_B0034 stem_loop 4913..4956 /label=stem_loop misc_feature 4902..4981 /label=BBa_B0010 terminator 4902..4981 /label=BBa_B0010 stem_loop 4997..5016 /label=T7 TE misc_feature 5023..5023 /label=stop polyA_site 5017..5030 /label=polya misc_feature 4990..5030 /label=BBa_B0012 terminator 4990..5030 /label=BBa_B0012 promoter 5039..5168 /label=BBa_I13453 misc_feature 3890..3927 /label=P(Cat) promoter 3893..3898 /label=-35 promoter 3916..3921 /label=-10 misc_feature 3890..3927 /label=BBa_I14033 misc_feature 3936..3947 /label=BBa_B0034 misc_feature 3940..3943 /label=conserved misc_feature 3954..4868 /label=BBa_C0080 CDS 3954..4829 /label=araC misc_feature 4830..4862 /label=LVA misc_feature 4869..4893 /label=barcode misc_feature 4902..4981 /label=BBa_B0010 stem_loop 4913..4956 /label=stem_loop misc_feature 4990..5030 /label=BBa_B0012 stem_loop 4997..5016 /label=T7 TE polyA_site 5017..5030 /label=polya misc_feature 5023..5023 /label=stop misc_feature 3936..5030 /label=BBa_K228006 misc_feature 5039..5168 /label=BBa_I13453 misc_feature 3890..5030 /label=BBa_K228008 promoter 5039..5168 /label=BBa_I13453 promoter 3890..5168 /label=BBa_K228009 misc_feature 5177..5312 /label=BBa_K228001 misc_feature 3890..5312 /label=BBa_K228012 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agtcacacag gaaagtacta gatgaccatg 1081 attaccgtgc actagaatac cattaacatt gctaagaacg acttctctga catcgaactg 1141 gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag 1201 ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag 1261 cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc 1321 ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc 1381 ggcaagcgcc cgacagcctt ccagttcctg taggaaatca agccggaagc cgtagcgtac 1441 atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct 1501 gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac 1561 cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac 1621 gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc 1681 ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc 1741 atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta 1801 gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc 1861 cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag 1921 ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg 1981 gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg 2041 tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg 2101 gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag 2161 cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg 2221 tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc 2281 agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc 2341 ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt 2401 aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt 2461 tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct 2521 gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca 2581 ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt 2641 gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt 2701 gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt 2761 cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag 2821 aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga agtagttacc 2881 gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg 2941 gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg 3001 ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag 3061 ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac 3121 atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg 3181 aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga 3241 gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg 3301 caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc 3361 ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct 3421 ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg 3481 tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc 3541 attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat 3601 gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct 3661 caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta 3721 gagtcggact tcgcgttcgc ataatactag agccaggcat caaataaaac gaaaggctca 3781 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 3841 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt ataaacgcag 3901 aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa 3961 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggct 4021 ctagtagtga tctacactag cactatcagt gttattaagc tactaaagcg tagttttcgt 4081 cgtttgcagc tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga 4141 actcgctcgg gctggccccg gtgcattttt taaatactcg cgagaaatag agttgatcgt 4201 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtagtgctc aaaagcagct 4261 tcgcctggct gatgcgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc 4321 ggaaaagatg tgacagacgc gacggcgaca ggcaaacatg ctgtgcgacg ctggcgatat 4381 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat 4441 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa 4501 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt 4561 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaacccgtat 4621 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa 4681 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg 4741 gcgggaacag caaaatatca cccggtcggc agacaaattc tcgtccctga tttttcacca 4801 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga 4861 taaaaaaatc gagataacca ttggcctcaa tcggcgttaa acccgccacc agatgggcgt 4921 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatctag tatttctcct 4981 ctttctctag tatgtttcat tatggtgaaa gttggaacct cttacgtgcc tactagagac 5041 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta 5101 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata ccgttttttt 5161 gggctagcta ctagagcaat tcggagagat gccggagcgg ctgaacggac cggtctctaa 5221 aaccggagta ggggcaactc taccgggggt tcaaatcccc ctctctccgc cactacagat 5281 ccttagcgaa agctaaggat tttttttaag ct //