##gff-version 3
##sequence-region BBa_K228040 1 5312
BBa_K228040	.	engineered_region	1	3881	.	+	0	ID=BBa_K228220;Name=BBa_K228220
BBa_K228040	.	engineered_region	1	1044	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_K228220
BBa_K228040	.	engineered_region	46	981	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_K228040	.	CDS	64	819	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K228040	.	CDS	64	813	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K228040	.	engineered_region	64	819	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K228040	.	engineered_region	64	65	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K228040	.	point_mutation	237	237	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K228040	.	engineered_tag	820	844	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K228040	.	point_mutation	555	555	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K228040	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K228040	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228040	.	terminator	941	981	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K228040	.	stem_loop	948	967	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228040	.	stop_codon	974	974	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228040	.	engineered_region	941	981	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228040	.	polyA_site	968	981	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228040	.	terminator	853	932	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K228040	.	stem_loop	864	907	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228040	.	engineered_region	853	932	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228040	.	promoter	990	1044	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_K228040	.	promoter	1009	1014	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K228040	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K228040	.	engineered_region	990	1044	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K228040	.	start_codon	1042	1042	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K228040	.	promoter	1031	1036	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K228040	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_K228040	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K228040	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K228040	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K228040	.	engineered_region	1053	3881	.	+	0	ID=BBa_K228202;Name=BBa_K228202;Parent=BBa_K228220
BBa_K228040	.	CDS	1072	3744	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228202
BBa_K228040	.	CDS	1072	3744	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228040	.	ribosome_entry_site	1053	1065	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K228202
BBa_K228040	.	ribosome_entry_site	1053	1065	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K228040	.	ribosome_entry_site	1059	1062	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K228040	.	engineered_region	1053	1065	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K228040	.	terminator	3753	3832	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228202
BBa_K228040	.	stem_loop	3764	3807	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228040	.	engineered_region	3753	3832	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228040	.	terminator	3841	3881	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228202
BBa_K228040	.	stem_loop	3848	3867	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228040	.	stop_codon	3874	3874	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228040	.	engineered_region	3841	3881	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228040	.	polyA_site	3868	3881	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228040	.	engineered_region	3890	5312	.	+	0	ID=BBa_K228012;Name=BBa_K228012
BBa_K228040	.	promoter	3890	5168	.	+	0	ID=BBa_K228009;Name=BBa_K228009;Parent=BBa_K228012
BBa_K228040	.	promoter	5039	5168	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228040	.	engineered_region	3890	5030	.	+	0	ID=BBa_K228008;Name=BBa_K228008;Parent=BBa_K228009
BBa_K228040	.	operator	3890	3927	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K228040	.	CDS	3954	4829	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K228040	.	engineered_region	3936	3947	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K228040	.	engineered_region	4869	4893	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K228040	.	engineered_region	3890	3927	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K228040	.	engineered_region	4990	5030	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K228040	.	polyA_site	5017	5030	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K228040	.	stop_codon	5023	5023	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K228040	.	promoter	3916	3921	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K228040	.	engineered_region	3954	4868	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K228040	.	engineered_region	3936	5030	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K228040	.	engineered_region	5039	5168	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K228040	.	promoter	3893	3898	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K228040	.	tag	4830	4862	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K228040	.	engineered_region	4902	4981	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K228040	.	stem_loop	4997	5016	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K228040	.	conserved_region	3940	3943	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K228040	.	stem_loop	4913	4956	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K228040	.	promoter	5039	5168	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228040	.	mature_transcript_region	5177	5312	.	+	0	ID=BBa_K228001;Name=BBa_K228001;Parent=BBa_K228012
BBa_K228040	.	terminator	4902	4981	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228040	.	stem_loop	4913	4956	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228040	.	engineered_region	4902	4981	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228040	.	ribosome_entry_site	3936	3947	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K228040	.	conserved_region	3940	3943	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228040	.	terminator	4990	5030	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228040	.	stem_loop	4997	5016	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228040	.	stop_codon	5023	5023	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228040	.	engineered_region	4990	5030	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228040	.	polyA_site	5017	5030	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K228040
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagtcacacaggaaagtactagatgaccatgattaccgtgcactagaataccattaa
cattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgt
ttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtc
aacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacg
catcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaag
ccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctg
tagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaa
gaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgac
atgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgct
gcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctga
gactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttc
caaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctgg
cgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaa
cattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgt
ccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctc
tcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagtt
catgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtt
tacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcg
gtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcat
caagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaa
gattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctccc
ttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgc
ggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagca
gacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagc
tgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgct
ggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaag
ggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtgg
tagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactgg
agagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcct
attcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgaga
ttgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgt
agtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgct
gcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgacc
agttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtga
catcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcac
cgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtg
atctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctac
atcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaa
tagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcct
ggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacgg
cgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcc
tcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagca
gatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatg
cggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtag
gcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctg
gcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggt
gagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgtt
aaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttct
cctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacg
gcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctc
tactgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggt
ctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagc
taaggattttttttaagct