##gff-version 3
##sequence-region BBa_K228041 1 5310
BBa_K228041	.	terminator	4900	4979	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228041	.	stem_loop	4911	4954	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228041	.	engineered_region	4900	4979	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228041	.	ribosome_entry_site	3934	3945	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K228041	.	conserved_region	3938	3941	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228041	.	engineered_region	1	3879	.	+	0	ID=BBa_K228221;Name=BBa_K228221
BBa_K228041	.	engineered_region	1	1044	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_K228221
BBa_K228041	.	engineered_region	46	981	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_K228041	.	CDS	64	819	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K228041	.	CDS	64	813	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K228041	.	engineered_region	64	819	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K228041	.	engineered_region	64	65	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K228041	.	point_mutation	237	237	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K228041	.	engineered_tag	820	844	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K228041	.	point_mutation	555	555	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K228041	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K228041	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228041	.	terminator	941	981	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K228041	.	stem_loop	948	967	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228041	.	stop_codon	974	974	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228041	.	engineered_region	941	981	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228041	.	polyA_site	968	981	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228041	.	terminator	853	932	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K228041	.	stem_loop	864	907	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228041	.	engineered_region	853	932	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228041	.	promoter	990	1044	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_K228041	.	promoter	1009	1014	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K228041	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K228041	.	engineered_region	990	1044	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K228041	.	start_codon	1042	1042	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K228041	.	promoter	1031	1036	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K228041	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_K228041	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K228041	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K228041	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K228041	.	engineered_region	1053	3879	.	+	0	ID=BBa_K228203;Name=BBa_K228203;Parent=BBa_K228221
BBa_K228041	.	CDS	1070	3742	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228203
BBa_K228041	.	CDS	1070	3742	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228041	.	terminator	3839	3879	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228203
BBa_K228041	.	stem_loop	3846	3865	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228041	.	stop_codon	3872	3872	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228041	.	engineered_region	3839	3879	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228041	.	polyA_site	3866	3879	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228041	.	terminator	3751	3830	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228203
BBa_K228041	.	stem_loop	3762	3805	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228041	.	engineered_region	3751	3830	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228041	.	ribosome_entry_site	1053	1063	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_K228203
BBa_K228041	.	ribosome_entry_site	1053	1063	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K228041	.	ribosome_entry_site	1059	1062	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K228041	.	engineered_region	1053	1063	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K228041	.	engineered_region	3888	5310	.	+	0	ID=BBa_K228012;Name=BBa_K228012
BBa_K228041	.	promoter	3888	5166	.	+	0	ID=BBa_K228009;Name=BBa_K228009;Parent=BBa_K228012
BBa_K228041	.	promoter	5037	5166	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228041	.	engineered_region	3888	5028	.	+	0	ID=BBa_K228008;Name=BBa_K228008;Parent=BBa_K228009
BBa_K228041	.	operator	3888	3925	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K228041	.	CDS	3952	4827	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K228041	.	engineered_region	3934	3945	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K228041	.	engineered_region	4867	4891	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K228041	.	engineered_region	3888	3925	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K228041	.	engineered_region	4988	5028	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K228041	.	polyA_site	5015	5028	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K228041	.	stop_codon	5021	5021	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K228041	.	promoter	3914	3919	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K228041	.	engineered_region	3952	4866	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K228041	.	engineered_region	3934	5028	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K228041	.	engineered_region	5037	5166	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K228041	.	promoter	3891	3896	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K228041	.	tag	4828	4860	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K228041	.	engineered_region	4900	4979	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K228041	.	stem_loop	4995	5014	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K228041	.	conserved_region	3938	3941	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K228041	.	stem_loop	4911	4954	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K228041	.	promoter	5037	5166	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228041	.	mature_transcript_region	5175	5310	.	+	0	ID=BBa_K228001;Name=BBa_K228001;Parent=BBa_K228012
BBa_K228041	.	terminator	4988	5028	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228041	.	stem_loop	4995	5014	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228041	.	stop_codon	5021	5021	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228041	.	engineered_region	4988	5028	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228041	.	polyA_site	5015	5028	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K228041
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagtcacacaggactactagatgaccatgattaccgtgcactagaataccattaaca
ttgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgttt
agctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaa
cttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgca
tcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagcc
ggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgta
gcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaaga
aaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacat
gctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgc
atcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgaga
ctatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttcca
accttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcg
ctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaaca
ttgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtcc
ggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctc
accgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttca
tgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgttta
cgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggt
aaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatca
agttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaaga
ttctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctccctt
ccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcgg
ttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcaga
cgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctg
ggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctgg
cttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaaggg
tctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggta
gctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggag
agattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctat
tcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagatt
gatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtag
tgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgc
gaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccag
ttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgaca
tcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccg
acaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgat
ctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacat
cattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaata
gagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctgg
ctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcg
acaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctc
gcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcaga
tttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcg
gctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggc
gcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggc
gggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtga
gattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaa
acccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcc
tctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggc
gtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctcta
ctgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggtct
ctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagcta
aggattttttttaagct