##gff-version 3
##sequence-region BBa_K228043 1 5314
BBa_K228043	.	engineered_region	1	3883	.	+	0	ID=BBa_K228223;Name=BBa_K228223
BBa_K228043	.	engineered_region	1	1044	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_K228223
BBa_K228043	.	engineered_region	46	981	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_K228043	.	terminator	941	981	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K228043	.	stop_codon	974	974	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228043	.	polyA_site	968	981	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228043	.	stem_loop	948	967	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228043	.	engineered_region	941	981	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228043	.	CDS	64	819	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K228043	.	engineered_tag	820	844	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K228043	.	engineered_region	64	819	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K228043	.	engineered_region	64	65	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K228043	.	point_mutation	237	237	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K228043	.	CDS	64	813	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K228043	.	point_mutation	555	555	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K228043	.	terminator	853	932	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K228043	.	engineered_region	853	932	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228043	.	stem_loop	864	907	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228043	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K228043	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228043	.	promoter	990	1044	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_K228043	.	start_codon	1042	1042	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K228043	.	promoter	1031	1036	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K228043	.	promoter	1009	1014	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K228043	.	engineered_region	990	1044	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K228043	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K228043	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_K228043	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K228043	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K228043	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K228043	.	engineered_region	1053	3883	.	+	0	ID=BBa_K228205;Name=BBa_K228205;Parent=BBa_K228223
BBa_K228043	.	terminator	3843	3883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228205
BBa_K228043	.	stop_codon	3876	3876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228043	.	polyA_site	3870	3883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228043	.	stem_loop	3850	3869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228043	.	engineered_region	3843	3883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228043	.	terminator	3755	3834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228205
BBa_K228043	.	engineered_region	3755	3834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228043	.	stem_loop	3766	3809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228043	.	ribosome_entry_site	1053	1067	.	+	0	ID=BBa_J44001;Name=BBa_J44001;Parent=BBa_K228205
BBa_K228043	.	ribosome_entry_site	1053	1067	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_K228043	.	CDS	1074	3746	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228205
BBa_K228043	.	CDS	1074	3746	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228043	.	terminator	4904	4983	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228043	.	engineered_region	4904	4983	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228043	.	stem_loop	4915	4958	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228043	.	engineered_region	3892	5314	.	+	0	ID=BBa_K228012;Name=BBa_K228012
BBa_K228043	.	promoter	3892	5170	.	+	0	ID=BBa_K228009;Name=BBa_K228009;Parent=BBa_K228012
BBa_K228043	.	engineered_region	3892	5032	.	+	0	ID=BBa_K228008;Name=BBa_K228008;Parent=BBa_K228009
BBa_K228043	.	polyA_site	5019	5032	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K228043	.	promoter	3918	3923	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K228043	.	engineered_region	3938	3949	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K228043	.	engineered_region	3938	5032	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K228043	.	conserved_region	3942	3945	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K228043	.	engineered_region	4904	4983	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K228043	.	stop_codon	5025	5025	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K228043	.	engineered_region	5041	5170	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K228043	.	stem_loop	4915	4958	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K228043	.	tag	4832	4864	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K228043	.	stem_loop	4999	5018	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K228043	.	operator	3892	3929	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K228043	.	engineered_region	4992	5032	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K228043	.	engineered_region	3956	4870	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K228043	.	promoter	3895	3900	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K228043	.	engineered_region	3892	3929	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K228043	.	CDS	3956	4831	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K228043	.	engineered_region	4871	4895	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K228043	.	promoter	5041	5170	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228043	.	promoter	5041	5170	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228043	.	mature_transcript_region	5179	5314	.	+	0	ID=BBa_K228001;Name=BBa_K228001;Parent=BBa_K228012
BBa_K228043	.	ribosome_entry_site	3938	3949	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K228043	.	conserved_region	3942	3945	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228043	.	terminator	4992	5032	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228043	.	stop_codon	5025	5025	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228043	.	polyA_site	5019	5032	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228043	.	stem_loop	4999	5018	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228043	.	engineered_region	4992	5032	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K228043
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagtttctcctctttaattactagatgaccatgattaccgtgcactagaataccatt
aacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagc
gtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcg
tcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgca
cgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatca
agccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggc
tgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttc
aagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctg
acatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacg
ctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactct
gagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgt
tccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctct
ggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgatt
aacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcatt
gtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggc
tctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgag
ttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtg
tttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaat
cggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgc
atcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagc
aagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctc
ccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgc
gcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaag
cagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaa
gctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacg
ctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggca
agggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggt
ggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagact
ggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagc
ctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcga
gattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagact
gtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacg
ctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacga
ccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgt
gacatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttc
accgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtag
tgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggct
acatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgaga
aatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgc
ctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgac
ggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaag
cctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaag
cagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaa
tgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagt
aggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcc
tggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatg
gtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcg
ttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtattt
ctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgca
cggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactc
tctactgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccg
gtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaa
gctaaggattttttttaagct