BBa_K228044
type: ComponentDefinition
persistent identity: BBa K228044
display id: BBa_K228044
version: 1
was generated by: iGEM to SBOL conversion
description: AND Gate LuxR-rbs(J61100)-T7ptag-AraC-SupD
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: PKU_Beijing iGEM 2009 Team
owned by: james
owned by: myers
mutable description: {{PKU_Beijing2009_AND_GATE_FAMILY}} This is a AND GATE in the Ecoli stain DH5a. Use arabinose to induce the transcription of SupD and use IPTG to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter. This gate is only one of nine gates(K228038-K228045), which are different in the RBS of T7ptag. For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
discontinued: false
dominant: false
experience: experience/Issues
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Gao Rencheng
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K228224
component: BBa_B0010
component: BBa_B0034
component: BBa_K228012
component: BBa_B0012
sequence annotation: BBa_K228224
sequence annotation: BBa_K228012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K228044_sequence/1

BBa_K228224
type: Component
persistent identity: component2300069
display id: component2300069
version: 1
top level: BBa_K228044
definition: BBa_K228224
access: public

BBa_B0010
type: Component
persistent identity: component2300083
display id: component2300083
version: 1
top level: BBa_K228044
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component2300076
display id: component2300076
version: 1
top level: BBa_K228044
definition: BBa_B0034
access: public

BBa_K228012
type: Component
persistent identity: component2300092
display id: component2300092
version: 1
top level: BBa_K228044
definition: BBa_K228012
access: public

BBa_B0012
type: Component
persistent identity: component2300085
display id: component2300085
version: 1
top level: BBa_K228044
definition: BBa_B0012
access: public

BBa_K228224
type: SequenceAnnotation
persistent identity: annotation2300069
display id: annotation2300069
version: 1
top level: BBa_K228044
location: https://synbiohub.org/public/igem/BBa_K228044/annotation2300069/range2300069/1
component: BBa_K228224

https://synbiohub.org/public/igem/BBa_K228044/annotation2300069/range2300069/1
type: Range
persistent identity: range2300069
display id: range2300069
version: 1
top level: BBa_K228044
direction: direction/reverse
start: 1
end: 3880
orientation: inline

BBa_K228012
type: SequenceAnnotation
persistent identity: annotation2300092
display id: annotation2300092
version: 1
top level: BBa_K228044
location: https://synbiohub.org/public/igem/BBa_K228044/annotation2300092/range2300092/1
component: BBa_K228012

https://synbiohub.org/public/igem/BBa_K228044/annotation2300092/range2300092/1
type: Range
persistent identity: range2300092
display id: range2300092
version: 1
top level: BBa_K228044
direction: direction/reverse
start: 3889
end: 5311
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2300076
display id: annotation2300076
version: 1
top level: BBa_K228044
location: https://synbiohub.org/public/igem/BBa_K228044/annotation2300076/range2300076/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K228044/annotation2300076/range2300076/1
type: Range
persistent identity: range2300076
display id: range2300076
version: 1
top level: BBa_K228044
direction: direction/reverse
start: 3935
end: 3946
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2300083
display id: annotation2300083
version: 1
top level: BBa_K228044
location: https://synbiohub.org/public/igem/BBa_K228044/annotation2300083/range2300083/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K228044/annotation2300083/range2300083/1
type: Range
persistent identity: range2300083
display id: range2300083
version: 1
top level: BBa_K228044
direction: direction/forward
start: 4901
end: 4980
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2300085
display id: annotation2300085
version: 1
top level: BBa_K228044
location: https://synbiohub.org/public/igem/BBa_K228044/annotation2300085/range2300085/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K228044/annotation2300085/range2300085/1
type: Range
persistent identity: range2300085
display id: range2300085
version: 1
top level: BBa_K228044
direction: direction/forward
start: 4989
end: 5029
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K228224
type: ComponentDefinition
persistent identity: BBa K228224
display id: BBa_K228224
version: 1
was generated by: iGEM to SBOL conversion
description: LuxR->rbs->T7ptag->Terminator
created: 2009-09-19T11:00:00Z
modified: 2015-05-08T01:11:33Z
mutable provenance: Built by joining different standardized biobricks together
owned by: james
owned by: myers
mutable description: This is a branch of the AND GATE built by Peking University 2009 iGEM Team. The addition of homoserine lactone can induce the transcription of T7ptag. However, if the SupD tRNA is absent, T7ptag might not be translated into functional T7 polymerase normally because of some amber stop codon mutations in it. We also have constructed a few similar parts with various RBS, which would make the expression regulation of T7ptag gene more convenient by choosing different parts.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4413
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Guosheng Zhang
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K228206
component: BBa_J09855
sequence annotation: BBa_J09855
sequence annotation: BBa_K228206
sequence: https://synbiohub.org/public/igem/BBa_K228224_sequence/1

BBa_K228206
type: Component
persistent identity: component2285589
display id: component2285589
version: 1
top level: BBa_K228224
definition: BBa_K228206
access: public

BBa_J09855
type: Component
persistent identity: component2285579
display id: component2285579
version: 1
top level: BBa_K228224
definition: BBa_J09855
access: public

BBa_J09855
type: SequenceAnnotation
persistent identity: annotation2285579
display id: annotation2285579
version: 1
top level: BBa_K228224
location: https://synbiohub.org/public/igem/BBa_K228224/annotation2285579/range2285579/1
component: BBa_J09855

https://synbiohub.org/public/igem/BBa_K228224/annotation2285579/range2285579/1
type: Range
persistent identity: range2285579
display id: range2285579
version: 1
top level: BBa_K228224
direction: direction/reverse
start: 1
end: 1044
orientation: inline

BBa_K228206
type: SequenceAnnotation
persistent identity: annotation2285589
display id: annotation2285589
version: 1
top level: BBa_K228224
location: https://synbiohub.org/public/igem/BBa_K228224/annotation2285589/range2285589/1
component: BBa_K228206

https://synbiohub.org/public/igem/BBa_K228224/annotation2285589/range2285589/1
type: Range
persistent identity: range2285589
display id: range2285589
version: 1
top level: BBa_K228224
direction: direction/reverse
start: 1053
end: 3880
orientation: inline

BBa_K228012
type: ComponentDefinition
persistent identity: BBa K228012
display id: BBa_K228012
version: 1
was generated by: iGEM to SBOL conversion
description: AraC-Pbad-SupD
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: PKU_Beijing iGEM 2009 team
owned by: james
owned by: myers
mutable description: The transcription of SupD-tRNA is regulated by the Pbad promoter(<partinfo>BBa_I13453</partinfo>). Arabinose can activate the transciprtion by binding to AraC regulatory protein.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Gao Rencheng
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K228001
component: BBa_K228009
sequence annotation: BBa_K228009
sequence annotation: BBa_K228001
sequence: https://synbiohub.org/public/igem/BBa_K228012_sequence/1

BBa_K228001
type: Component
persistent identity: component2246597
display id: component2246597
version: 1
top level: BBa_K228012
definition: BBa_K228001
access: public

BBa_K228009
type: Component
persistent identity: component2246596
display id: component2246596
version: 1
top level: BBa_K228012
definition: BBa_K228009
access: public

BBa_K228009
type: SequenceAnnotation
persistent identity: annotation2246596
display id: annotation2246596
version: 1
top level: BBa_K228012
location: https://synbiohub.org/public/igem/BBa_K228012/annotation2246596/range2246596/1
component: BBa_K228009

https://synbiohub.org/public/igem/BBa_K228012/annotation2246596/range2246596/1
type: Range
persistent identity: range2246596
display id: range2246596
version: 1
top level: BBa_K228012
direction: direction/reverse
start: 1
end: 1279
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2246597
display id: annotation2246597
version: 1
top level: BBa_K228012
location: https://synbiohub.org/public/igem/BBa_K228012/annotation2246597/range2246597/1
component: BBa_K228001

https://synbiohub.org/public/igem/BBa_K228012/annotation2246597/range2246597/1
type: Range
persistent identity: range2246597
display id: range2246597
version: 1
top level: BBa_K228012
direction: direction/reverse
start: 1288
end: 1423
orientation: inline

BBa_J09855
type: ComponentDefinition
persistent identity: BBa J09855
display id: BBa_J09855
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive luxR device with pLuxR
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:21Z
mutable provenance: This part was constructed from registry biobricks.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: I14032.I0462.R0062
discontinued: false
dominant: true
experience: experience/None
group u list: _72_40_
m user id: 0
owner id: 475
owning group id: 72
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This is a universal part designed to allow induction by HSL of whatever is assembled downstream of the part.
star: true
creator: Jon Badalamenti
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0062
component: BBa_I0462
component: BBa_I14032
sequence annotation: BBa_I14032
sequence annotation: BBa_I0462
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_J09855_sequence/1

BBa_R0062
type: Component
persistent identity: component2260447
display id: component2260447
version: 1
top level: BBa_J09855
definition: lux pR
access: public

BBa_I0462
type: Component
persistent identity: component2260445
display id: component2260445
version: 1
top level: BBa_J09855
definition: LuxR
access: public

BBa_I14032
type: Component
persistent identity: component2260430
display id: component2260430
version: 1
top level: BBa_J09855
definition: P(Lac) IQ
access: public

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2260430
display id: annotation2260430
version: 1
top level: BBa_J09855
location: https://synbiohub.org/public/igem/BBa_J09855/annotation2260430/range2260430/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_J09855/annotation2260430/range2260430/1
type: Range
persistent identity: range2260430
display id: range2260430
version: 1
top level: BBa_J09855
direction: direction/reverse
start: 1
end: 37
orientation: inline

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2260445
display id: annotation2260445
version: 1
top level: BBa_J09855
location: https://synbiohub.org/public/igem/BBa_J09855/annotation2260445/range2260445/1
component: BBa_I0462

https://synbiohub.org/public/igem/BBa_J09855/annotation2260445/range2260445/1
type: Range
persistent identity: range2260445
display id: range2260445
version: 1
top level: BBa_J09855
direction: direction/reverse
start: 46
end: 981
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2260447
display id: annotation2260447
version: 1
top level: BBa_J09855
location: https://synbiohub.org/public/igem/BBa_J09855/annotation2260447/range2260447/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_J09855/annotation2260447/range2260447/1
type: Range
persistent identity: range2260447
display id: range2260447
version: 1
top level: BBa_J09855
direction: direction/forward
start: 990
end: 1044
orientation: inline

BBa_K228001
type: ComponentDefinition
persistent identity: BBa K228001
display id: BBa_K228001
version: 1
was generated by: iGEM to SBOL conversion
description: SupD-tRNA
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated. This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K228001_sequence/1

BBa_K228009
type: ComponentDefinition
persistent identity: BBa K228009
display id: BBa_K228009
version: 1
was generated by: iGEM to SBOL conversion
description: AraC protein(reversed sequence) and Pbad promoter
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
owned by: james
owned by: myers
mutable description: This part contains AraC protein generator(BBa_K228008) and Pbad promoter(BBa_I13453). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The maximum concentration of L-arabinose we tested is 10^-4 M.
star: false
creator: Gao Rencheng
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_K228008
component: BBa_I13453
sequence annotation: BBa_K228008
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K228009_sequence/1

BBa_K228008
type: Component
persistent identity: component2244233
display id: component2244233
version: 1
top level: BBa_K228009
definition: BBa_K228008
access: public

BBa_I13453
type: Component
persistent identity: component2244232
display id: component2244232
version: 1
top level: BBa_K228009
definition: BBa_I13453
access: public

BBa_I13453
type: Component
persistent identity: component2244234
display id: component2244234
version: 1
top level: BBa_K228009
definition: BBa_I13453
access: public

BBa_K228008
type: SequenceAnnotation
persistent identity: annotation2244233
display id: annotation2244233
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244233/range2244233/1
component: BBa_K228008

https://synbiohub.org/public/igem/BBa_K228009/annotation2244233/range2244233/1
type: Range
persistent identity: range2244233
display id: range2244233
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1
end: 1141
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2244232
display id: annotation2244232
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244232/range2244232/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228009/annotation2244232/range2244232/1
type: Range
persistent identity: range2244232
display id: range2244232
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2244234
display id: annotation2244234
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244234/range2244234/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228009/annotation2244234/range2244234/1
type: Range
persistent identity: range2244234
display id: range2244234
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_K228206
type: ComponentDefinition
persistent identity: BBa K228206
display id: BBa_K228206
version: 1
was generated by: iGEM to SBOL conversion
description: RBS->T7ptag->Terminator
created: 2009-09-15T11:00:00Z
modified: 2015-05-08T01:11:33Z
mutable provenance: Built from standardized parts
owned by: james
owned by: myers
mutable description: The part is RBS->T7ptag->Terminator. K228002 is added to the RBS BBa_J61100 to construct this part. We also have constructed a few similar parts with various RBS, which would make the expression regulation of T7ptag gene more convenient by choosing different parts.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4413
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Because the RBS is only about 10bp, so let the BBa_K228002 be the insert and the backbone with RBS be the vector would be more convenient.
star: false
creator: Guosheng Zhang
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0010
component: BBa_K228000
component: BBa_B0012
component: BBa_J61100
sequence annotation: BBa_J61100
sequence annotation: BBa_K228000
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K228206_sequence/1

BBa_B0010
type: Component
persistent identity: component2021285
display id: component2021285
version: 1
top level: BBa_K228206
definition: BBa_B0010
access: public

BBa_K228000
type: Component
persistent identity: component2021284
display id: component2021284
version: 1
top level: BBa_K228206
definition: BBa_K228000
access: public

BBa_B0012
type: Component
persistent identity: component2021287
display id: component2021287
version: 1
top level: BBa_K228206
definition: BBa_B0012
access: public

BBa_J61100
type: Component
persistent identity: component2021282
display id: component2021282
version: 1
top level: BBa_K228206
definition: BBa_J61100
access: public

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2021282
display id: annotation2021282
version: 1
top level: BBa_K228206
location: https://synbiohub.org/public/igem/BBa_K228206/annotation2021282/range2021282/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K228206/annotation2021282/range2021282/1
type: Range
persistent identity: range2021282
display id: range2021282
version: 1
top level: BBa_K228206
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K228000
type: SequenceAnnotation
persistent identity: annotation2021284
display id: annotation2021284
version: 1
top level: BBa_K228206
location: https://synbiohub.org/public/igem/BBa_K228206/annotation2021284/range2021284/1
component: BBa_K228000

https://synbiohub.org/public/igem/BBa_K228206/annotation2021284/range2021284/1
type: Range
persistent identity: range2021284
display id: range2021284
version: 1
top level: BBa_K228206
direction: direction/reverse
start: 19
end: 2691
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2021285
display id: annotation2021285
version: 1
top level: BBa_K228206
location: https://synbiohub.org/public/igem/BBa_K228206/annotation2021285/range2021285/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K228206/annotation2021285/range2021285/1
type: Range
persistent identity: range2021285
display id: range2021285
version: 1
top level: BBa_K228206
direction: direction/forward
start: 2700
end: 2779
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2021287
display id: annotation2021287
version: 1
top level: BBa_K228206
location: https://synbiohub.org/public/igem/BBa_K228206/annotation2021287/range2021287/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K228206/annotation2021287/range2021287/1
type: Range
persistent identity: range2021287
display id: range2021287
version: 1
top level: BBa_K228206
direction: direction/forward
start: 2788
end: 2828
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: iGEM to SBOL conversion
description: luxR Protein Generator
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0062
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
definition: luxr
access: public

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
top level: LuxR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
top level: LuxR
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
top level: LuxR
direction: direction/forward
start: 808
end: 887
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
top level: LuxR
direction: direction/forward
start: 896
end: 936
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: iGEM to SBOL conversion
description: promoter P(Lac) IQ
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, High Transcription
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 26
end: 31
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

BBa_K228000
type: ComponentDefinition
persistent identity: BBa K228000
display id: BBa_K228000
version: 1
was generated by: iGEM to SBOL conversion
description: T7ptag(T7polymerase with amber mutation)
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: The T7ptag is standardlized from the plasmid kindly provided by Voigt Lab.
owned by: james
owned by: myers
mutable description: T7ptag is a coding sequence of T7 polymerase with 2 Amber mutations. The transcription of T7ptag gene can only lead to the generation of its mRNA, further translation into T7 RNA polymerase is blocked because of the amber mutation. Only in the presence of the Amber mutation suppressor BBa_K228001 SupD-tRNA, it can be translated into functional T7 polymerase. T7ptag is a component of the AND Gate constructed by the Voigt Lab(UCSF)
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K228000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2017714
display id: annotation2017714
version: 1
top level: BBa_K228000
location: https://synbiohub.org/public/igem/BBa_K228000/annotation2017714/range2017714/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228000/annotation2017714/range2017714/1
type: Range
persistent identity: range2017714
display id: range2017714
version: 1
top level: BBa_K228000
direction: direction/forward
start: 1
end: 2673
orientation: inline

BBa_K228008
type: ComponentDefinition
persistent identity: BBa K228008
display id: BBa_K228008
version: 1
was generated by: iGEM to SBOL conversion
description: AraC regulatory protein
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
owned by: james
owned by: myers
mutable description: This part could be connected to a protein coding sequence so that the expression could be induced by L-arabinose
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Gao Rencheng
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_I14033
sequence annotation: P(Cat)
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_B0034
sequence annotation: BBa_K228006
sequence annotation: conserved
sequence annotation: araC
sequence annotation: BBa_C0080
sequence annotation: LVA
sequence annotation: barcode
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K228008_sequence/1

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation2020002
display id: annotation2020002
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020002/range2020002/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020002/range2020002/1
type: Range
persistent identity: range2020002
display id: range2020002
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 1
end: 38
orientation: inline

P(Cat)
type: SequenceAnnotation
persistent identity: annotation2019999
display id: annotation2019999
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2019999/range2019999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K228008/annotation2019999/range2019999/1
type: Range
persistent identity: range2019999
display id: range2019999
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2020000
display id: annotation2020000
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020000/range2020000/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228008/annotation2020000/range2020000/1
type: Range
persistent identity: range2020000
display id: range2020000
version: 1
top level: BBa_K228008
direction: direction/forward
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2020001
display id: annotation2020001
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020001/range2020001/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228008/annotation2020001/range2020001/1
type: Range
persistent identity: range2020001
display id: range2020001
version: 1
top level: BBa_K228008
direction: direction/forward
start: 27
end: 32
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2020003
display id: annotation2020003
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020003/range2020003/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020003/range2020003/1
type: Range
persistent identity: range2020003
display id: range2020003
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 47
end: 58
orientation: inline

BBa_K228006
type: SequenceAnnotation
persistent identity: annotation2020015
display id: annotation2020015
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020015/range2020015/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020015/range2020015/1
type: Range
persistent identity: range2020015
display id: range2020015
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 47
end: 1141
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2020004
display id: annotation2020004
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020004/range2020004/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K228008/annotation2020004/range2020004/1
type: Range
persistent identity: range2020004
display id: range2020004
version: 1
top level: BBa_K228008
direction: direction/forward
start: 51
end: 54
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2020006
display id: annotation2020006
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020006/range2020006/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228008/annotation2020006/range2020006/1
type: Range
persistent identity: range2020006
display id: range2020006
version: 1
top level: BBa_K228008
direction: direction/forward
start: 65
end: 940
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2020005
display id: annotation2020005
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020005/range2020005/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020005/range2020005/1
type: Range
persistent identity: range2020005
display id: range2020005
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 65
end: 979
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2020007
display id: annotation2020007
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020007/range2020007/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K228008/annotation2020007/range2020007/1
type: Range
persistent identity: range2020007
display id: range2020007
version: 1
top level: BBa_K228008
direction: direction/forward
start: 941
end: 973
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation2020008
display id: annotation2020008
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020008/range2020008/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020008/range2020008/1
type: Range
persistent identity: range2020008
display id: range2020008
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 980
end: 1004
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2020009
display id: annotation2020009
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020009/range2020009/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020009/range2020009/1
type: Range
persistent identity: range2020009
display id: range2020009
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1013
end: 1092
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2020010
display id: annotation2020010
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020010/range2020010/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K228008/annotation2020010/range2020010/1
type: Range
persistent identity: range2020010
display id: range2020010
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1024
end: 1067
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2020011
display id: annotation2020011
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020011/range2020011/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020011/range2020011/1
type: Range
persistent identity: range2020011
display id: range2020011
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1101
end: 1141
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2020012
display id: annotation2020012
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020012/range2020012/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K228008/annotation2020012/range2020012/1
type: Range
persistent identity: range2020012
display id: range2020012
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1108
end: 1127
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2020013
display id: annotation2020013
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020013/range2020013/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K228008/annotation2020013/range2020013/1
type: Range
persistent identity: range2020013
display id: range2020013
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1128
end: 1141
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2020014
display id: annotation2020014
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020014/range2020014/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K228008/annotation2020014/range2020014/1
type: Range
persistent identity: range2020014
display id: range2020014
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1134
end: 1134
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2020016
display id: annotation2020016
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020016/range2020016/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020016/range2020016/1
type: Range
persistent identity: range2020016
display id: range2020016
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

https://synbiohub.org/public/igem/BBa_K228044_sequence/1
type: Sequence
persistent identity: BBa K228044 sequence
display id: BBa_K228044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggggacatactagatgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228224_sequence/1
type: Sequence
persistent identity: BBa K228224 sequence
display id: BBa_K228224_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggggacatactagatgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228012_sequence/1
type: Sequence
persistent identity: BBa K228012 sequence
display id: BBa_K228012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J09855_sequence/1
type: Sequence
persistent identity: BBa J09855 sequence
display id: BBa_J09855_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228001_sequence/1
type: Sequence
persistent identity: BBa K228001 sequence
display id: BBa_K228001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228009_sequence/1
type: Sequence
persistent identity: BBa K228009 sequence
display id: BBa_K228009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228206_sequence/1
type: Sequence
persistent identity: BBa K228206 sequence
display id: BBa_K228206_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggacatactagatgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagatac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0462_sequence/1
type: Sequence
persistent identity: BBa I0462 sequence
display id: BBa_I0462_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14032_sequence/1
type: Sequence
persistent identity: BBa I14032 sequence
display id: BBa_I14032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61100_sequence/1
type: Sequence
persistent identity: BBa J61100 sequence
display id: BBa_J61100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228000_sequence/1
type: Sequence
persistent identity: BBa K228000 sequence
display id: BBa_K228000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228008_sequence/1
type: Sequence
persistent identity: BBa K228008 sequence
display id: BBa_K228008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z