LOCUS BBa_K228045 5311 bp DNA linear 26-Dec-2024 DEFINITION AND Gate LuxR-rbs(J61107)-T7ptag-AraC-SupD ACCESSION BBa_K228045 VERSION BBa_K228045.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 RBS 1053..1064 /label=BBa_J61107 CDS 1071..3743 /label=cds CDS 1071..3743 /label=BBa_K228000 stem_loop 3763..3806 /label=stem_loop misc_feature 3752..3831 /label=BBa_B0010 terminator 3752..3831 /label=BBa_B0010 stem_loop 3847..3866 /label=T7 TE misc_feature 3873..3873 /label=stop polyA_site 3867..3880 /label=polya misc_feature 3840..3880 /label=BBa_B0012 terminator 3840..3880 /label=BBa_B0012 misc_feature 1053..3880 /label=BBa_K228207 misc_feature 1..3880 /label=BBa_K228225 misc_feature 3939..3942 /label=conserved RBS 3935..3946 /label=BBa_B0034 stem_loop 4912..4955 /label=stem_loop misc_feature 4901..4980 /label=BBa_B0010 terminator 4901..4980 /label=BBa_B0010 stem_loop 4996..5015 /label=T7 TE misc_feature 5022..5022 /label=stop polyA_site 5016..5029 /label=polya misc_feature 4989..5029 /label=BBa_B0012 terminator 4989..5029 /label=BBa_B0012 promoter 5038..5167 /label=BBa_I13453 misc_feature 3889..3926 /label=P(Cat) promoter 3892..3897 /label=-35 promoter 3915..3920 /label=-10 misc_feature 3889..3926 /label=BBa_I14033 misc_feature 3935..3946 /label=BBa_B0034 misc_feature 3939..3942 /label=conserved misc_feature 3953..4867 /label=BBa_C0080 CDS 3953..4828 /label=araC misc_feature 4829..4861 /label=LVA misc_feature 4868..4892 /label=barcode misc_feature 4901..4980 /label=BBa_B0010 stem_loop 4912..4955 /label=stem_loop misc_feature 4989..5029 /label=BBa_B0012 stem_loop 4996..5015 /label=T7 TE polyA_site 5016..5029 /label=polya misc_feature 5022..5022 /label=stop misc_feature 3935..5029 /label=BBa_K228006 misc_feature 5038..5167 /label=BBa_I13453 misc_feature 3889..5029 /label=BBa_K228008 promoter 5038..5167 /label=BBa_I13453 promoter 3889..5167 /label=BBa_K228009 misc_feature 5176..5311 /label=BBa_K228001 misc_feature 3889..5311 /label=BBa_K228012 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agaaagaaga gacttactag atgaccatga 1081 ttaccgtgca ctagaatacc attaacattg ctaagaacga cttctctgac atcgaactgg 1141 ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct cgcgaacagt 1201 tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag atgtttgagc 1261 gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc atcactaccc 1321 tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg 1381 gcaagcgccc gacagccttc cagttcctgt aggaaatcaa gccggaagcc gtagcgtaca 1441 tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc gttcaggctg 1501 tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt atccgtgacc 1561 ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc gtagggcacg 1621 tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag ggtctactcg 1681 gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga gtacgctgca 1741 tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat gctggcgtag 1801 taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct atcgcaaccc 1861 gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt cctcctaagc 1921 cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg 1981 tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg cctgaggtgt 2041 acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa gtcctagcgg 2101 tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct gcgattgagc 2161 gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt 2221 ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca 2281 gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc atctggttcc 2341 cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta 2401 acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt 2461 actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg 2521 agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct aagtctccac 2581 tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg ttctgctttg 2641 agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg ctggcgtttg 2701 acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag gtaggtggtc 2761 gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt gttgctaaga 2821 aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa gtagttaccg 2881 tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact aaggcactgg 2941 ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca gtcatgacgc 3001 tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat accattcagc 3061 cagctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct gctggataca 3121 tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt gaagcaatga 3181 actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag aagactggag 3241 agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc cctgtgtggc 3301 aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt cagttccgct 3361 tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa caggagtctg 3421 gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag actgtagtgt 3481 gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc ttcggtacca 3541 ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt gacacatatg 3601 agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg cacgagtctc 3661 aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt gacatcttag 3721 agtcggactt cgcgttcgca taatactaga gccaggcatc aaataaaacg aaaggctcag 3781 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 3841 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagta taaacgcaga 3901 aaggcccacc cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac 3961 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggctc 4021 tagtagtgat ctacactagc actatcagtg ttattaagct actaaagcgt agttttcgtc 4081 gtttgcagct gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa 4141 ctcgctcggg ctggccccgg tgcatttttt aaatactcgc gagaaataga gttgatcgtc 4201 aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtagtgctca aaagcagctt 4261 cgcctggctg atgcgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg 4321 gaaaagatgt gacagacgcg acggcgacag gcaaacatgc tgtgcgacgc tggcgatatc 4381 aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc 4441 catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag 4501 cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg 4561 cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga aacccgtatt 4621 ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac 4681 ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg 4741 cgggaacagc aaaatatcac ccggtcggca gacaaattct cgtccctgat ttttcaccac 4801 cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat 4861 aaaaaaatcg agataaccat tggcctcaat cggcgttaaa cccgccacca gatgggcgtt 4921 aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatctagt atttctcctc 4981 tttctctagt atgtttcatt atggtgaaag ttggaacctc ttacgtgcct actagagaca 5041 ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag 5101 cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac cgtttttttg 5161 ggctagctac tagagcaatt cggagagatg ccggagcggc tgaacggacc ggtctctaaa 5221 accggagtag gggcaactct accgggggtt caaatccccc tctctccgcc actacagatc 5281 cttagcgaaa gctaaggatt ttttttaagc t //