##gff-version 3
##sequence-region BBa_K228045 1 5311
BBa_K228045	.	engineered_region	3889	5311	.	+	0	ID=BBa_K228012;Name=BBa_K228012
BBa_K228045	.	mature_transcript_region	5176	5311	.	+	0	ID=BBa_K228001;Name=BBa_K228001;Parent=BBa_K228012
BBa_K228045	.	promoter	3889	5167	.	+	0	ID=BBa_K228009;Name=BBa_K228009;Parent=BBa_K228012
BBa_K228045	.	promoter	5038	5167	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228045	.	promoter	5038	5167	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228045	.	engineered_region	3889	5029	.	+	0	ID=BBa_K228008;Name=BBa_K228008;Parent=BBa_K228009
BBa_K228045	.	engineered_region	3889	3926	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K228045	.	engineered_region	3935	5029	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K228045	.	engineered_region	3935	3946	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K228045	.	polyA_site	5016	5029	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K228045	.	stop_codon	5022	5022	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K228045	.	stem_loop	4912	4955	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K228045	.	engineered_region	4989	5029	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K228045	.	engineered_region	5038	5167	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K228045	.	engineered_region	4901	4980	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K228045	.	promoter	3915	3920	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K228045	.	conserved_region	3939	3942	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K228045	.	tag	4829	4861	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K228045	.	engineered_region	3953	4867	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K228045	.	promoter	3892	3897	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K228045	.	CDS	3953	4828	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K228045	.	stem_loop	4996	5015	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K228045	.	engineered_region	4868	4892	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K228045	.	operator	3889	3926	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K228045	.	terminator	4989	5029	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228045	.	polyA_site	5016	5029	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228045	.	stem_loop	4996	5015	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228045	.	stop_codon	5022	5022	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228045	.	engineered_region	4989	5029	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228045	.	engineered_region	1	3880	.	+	0	ID=BBa_K228225;Name=BBa_K228225
BBa_K228045	.	engineered_region	1053	3880	.	+	0	ID=BBa_K228207;Name=BBa_K228207;Parent=BBa_K228225
BBa_K228045	.	terminator	3840	3880	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228207
BBa_K228045	.	polyA_site	3867	3880	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228045	.	stem_loop	3847	3866	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228045	.	stop_codon	3873	3873	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228045	.	engineered_region	3840	3880	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228045	.	CDS	1071	3743	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228207
BBa_K228045	.	CDS	1071	3743	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228045	.	terminator	3752	3831	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228207
BBa_K228045	.	stem_loop	3763	3806	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228045	.	engineered_region	3752	3831	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228045	.	ribosome_entry_site	1053	1064	.	+	0	ID=BBa_J61107;Name=BBa_J61107;Parent=BBa_K228207
BBa_K228045	.	engineered_region	1	1044	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_K228225
BBa_K228045	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_K228045	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K228045	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K228045	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K228045	.	promoter	990	1044	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_K228045	.	promoter	1009	1014	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K228045	.	promoter	1031	1036	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K228045	.	engineered_region	990	1044	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K228045	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K228045	.	start_codon	1042	1042	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K228045	.	engineered_region	46	981	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_K228045	.	terminator	853	932	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K228045	.	stem_loop	864	907	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228045	.	engineered_region	853	932	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228045	.	CDS	64	819	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K228045	.	engineered_tag	820	844	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K228045	.	engineered_region	64	819	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K228045	.	CDS	64	813	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K228045	.	point_mutation	237	237	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K228045	.	engineered_region	64	65	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K228045	.	point_mutation	555	555	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K228045	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K228045	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228045	.	terminator	941	981	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K228045	.	polyA_site	968	981	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228045	.	stem_loop	948	967	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228045	.	stop_codon	974	974	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228045	.	engineered_region	941	981	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228045	.	terminator	4901	4980	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228045	.	stem_loop	4912	4955	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228045	.	engineered_region	4901	4980	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228045	.	ribosome_entry_site	3935	3946	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K228045	.	conserved_region	3939	3942	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K228045
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagaaagaagagacttactagatgaccatgattaccgtgcactagaataccattaac
attgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtt
tagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtca
acttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgc
atcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagc
cggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgt
agcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaag
aaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgaca
tgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctg
catcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgag
actatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttcc
aaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggc
gctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaac
attgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtc
cggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctct
caccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttc
atgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgttt
acgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcgg
taaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatc
aagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaag
attctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctccct
tccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcg
gttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcag
acgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagct
gggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctg
gcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagg
gtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggt
agctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactgga
gagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagccta
ttcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagat
tgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgta
gtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctg
cgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgacca
gttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgac
atcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcacc
gacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtga
tctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctaca
tcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaat
agagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctg
gctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggc
gacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcct
cgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcag
atttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgc
ggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtagg
cgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctgg
cgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtg
agattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgtta
aacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctc
ctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacgg
cgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctct
actgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggtc
tctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagct
aaggattttttttaagct