LOCUS       BBa_K228098              930 bp    DNA     linear       22-Dec-2024
DEFINITION  T7 promoter+medium RBS+GFP
ACCESSION   BBa_K228098
VERSION     BBa_K228098.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     RBS             55..67
                     /label=RBS-3\Weak
     RBS             61..64
                     /label=RBS
     misc_feature    55..67
                     /label=BBa_B0032
     RBS             55..67
                     /label=BBa_B0032
     CDS             74..793
                     /label=GFP protein
     CDS             74..793
                     /label=BBa_E0040
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtcacac
       61 aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
      121 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      181 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      241 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
      301 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      361 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      421 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      481 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      541 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      601 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      661 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      721 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
      781 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata
//