LOCUS       BBa_K228101             3039 bp    DNA     linear       25-Nov-2024
DEFINITION  LacP(R0010) + RBS(B0030) + T7ptag(K228000) + terminator(B0015)
ACCESSION   BBa_K228101
VERSION     BBa_K228101.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..2902
                     /label=cds
     CDS             230..2902
                     /label=BBa_K228000
     stem_loop       2922..2965
                     /label=stem_loop
     misc_feature    2911..2990
                     /label=BBa_B0010
     terminator      2911..2990
                     /label=BBa_B0010
     stem_loop       3006..3025
                     /label=T7 TE
     misc_feature    3032..3032
                     /label=stop
     polyA_site      3026..3039
                     /label=polya
     misc_feature    2999..3039
                     /label=BBa_B0012
     terminator      2999..3039
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgaccatgat
      241 taccgtgcac tagaatacca ttaacattgc taagaacgac ttctctgaca tcgaactggc
      301 tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt
      361 ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg
      421 tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct
      481 actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg
      541 caagcgcccg acagccttcc agttcctgta ggaaatcaag ccggaagccg tagcgtacat
      601 caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt
      661 agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct
      721 tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt
      781 ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg
      841 tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat
      901 cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt
      961 aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg
     1021 tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc
     1081 gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt
     1141 gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta
     1201 caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt
     1261 cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg
     1321 tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg
     1381 gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag
     1441 ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc
     1501 ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa
     1561 cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta
     1621 ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga
     1681 gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact
     1741 ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga
     1801 gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga
     1861 cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg
     1921 cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa
     1981 agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt
     2041 gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta aggcactggc
     2101 tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag tcatgacgct
     2161 ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata ccattcagcc
     2221 agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg ctggatacat
     2281 ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa
     2341 ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga agactggaga
     2401 gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca
     2461 ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc agttccgctt
     2521 acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac aggagtctgg
     2581 tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga ctgtagtgtg
     2641 ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct tcggtaccat
     2701 tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg acacatatga
     2761 gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc acgagtctca
     2821 attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg acatcttaga
     2881 gtcggacttc gcgttcgcat aatactagag ccaggcatca aataaaacga aaggctcagt
     2941 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     3001 acactggctc accttcgggt gggcctttct gcgtttata
//