LOCUS       BBa_K228102             3037 bp    DNA     linear       25-Nov-2024
DEFINITION  LacP(R0010) + RBS(B0032) + T7ptag(K228000) + terminator(B0015)
ACCESSION   BBa_K228102
VERSION     BBa_K228102.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..2900
                     /label=cds
     CDS             228..2900
                     /label=BBa_K228000
     stem_loop       2920..2963
                     /label=stem_loop
     misc_feature    2909..2988
                     /label=BBa_B0010
     terminator      2909..2988
                     /label=BBa_B0010
     stem_loop       3004..3023
                     /label=T7 TE
     misc_feature    3030..3030
                     /label=stop
     polyA_site      3024..3037
                     /label=polya
     misc_feature    2997..3037
                     /label=BBa_B0012
     terminator      2997..3037
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg accatgatta
      241 ccgtgcacta gaataccatt aacattgcta agaacgactt ctctgacatc gaactggctg
      301 ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc gaacagttgg
      361 cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc
      421 aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac
      481 tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca
      541 agcgcccgac agccttccag ttcctgtagg aaatcaagcc ggaagccgta gcgtacatca
      601 ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag
      661 caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg
      721 aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct
      781 acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg
      841 gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg
      901 agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag
      961 gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg
     1021 caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct cctaagccgt
     1081 ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc
     1141 gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca
     1201 aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg
     1261 ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg
     1321 aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga
     1381 aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc
     1441 ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt
     1501 acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg
     1561 atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag gaaggttact
     1621 actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc
     1681 gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg
     1741 agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt
     1801 acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg
     1861 ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg
     1921 cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag
     1981 tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga
     2041 ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg
     2101 gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg
     2161 cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag
     2221 ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg
     2281 ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact
     2341 ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga
     2401 ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg
     2461 aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttac
     2521 agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta
     2581 tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg
     2641 cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc
     2701 cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt
     2761 cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat
     2821 tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttagagt
     2881 cggacttcgc gttcgcataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2941 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3001 actggctcac cttcgggtgg gcctttctgc gtttata
//