LOCUS       BBa_K228103             3036 bp    DNA     linear       22-Dec-2024
DEFINITION  LacP(R0010) + RBS(B0034) + T7ptag(K228000) + terminator(B0015)
ACCESSION   BBa_K228103
VERSION     BBa_K228103.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..2899
                     /label=cds
     CDS             227..2899
                     /label=BBa_K228000
     stem_loop       2919..2962
                     /label=stem_loop
     misc_feature    2908..2987
                     /label=BBa_B0010
     terminator      2908..2987
                     /label=BBa_B0010
     stem_loop       3003..3022
                     /label=T7 TE
     misc_feature    3029..3029
                     /label=stop
     polyA_site      3023..3036
                     /label=polya
     misc_feature    2996..3036
                     /label=BBa_B0012
     terminator      2996..3036
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ccatgattac
      241 cgtgcactag aataccatta acattgctaa gaacgacttc tctgacatcg aactggctgc
      301 tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc
      361 ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagcgtca
      421 acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact
      481 ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa
      541 gcgcccgaca gccttccagt tcctgtagga aatcaagccg gaagccgtag cgtacatcac
      601 cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc
      661 aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttga
      721 agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta
      781 caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg
      841 cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga
      901 gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg
      961 tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc
     1021 aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg
     1081 gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg
     1141 tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa
     1201 agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc
     1261 caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga
     1321 agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa
     1381 acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct
     1441 tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta
     1501 caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga
     1561 tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta
     1621 ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg
     1681 catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga
     1741 gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta
     1801 cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg
     1861 gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc
     1921 ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt
     1981 caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac
     2041 cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg
     2101 tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc
     2161 ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc
     2221 tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc
     2281 taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg
     2341 gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat
     2401 tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga
     2461 atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca
     2521 gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat
     2581 cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc
     2641 acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc
     2701 ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc
     2761 ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt
     2821 ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc
     2881 ggacttcgcg ttcgcataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     2941 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     3001 ctggctcacc ttcgggtggg cctttctgcg tttata
//