LOCUS       BBa_K228108             3035 bp    DNA     linear       25-Nov-2024
DEFINITION  LacP(R0010) + RBS(B0033) + T7ptag(K228000) + terminator(B0015)
ACCESSION   BBa_K228108
VERSION     BBa_K228108.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..219
                     /label=RBS-4\Weaker
     RBS             215..218
                     /label=RBS
     misc_feature    209..219
                     /label=BBa_B0033
     RBS             209..219
                     /label=BBa_B0033
     CDS             226..2898
                     /label=cds
     CDS             226..2898
                     /label=BBa_K228000
     stem_loop       2918..2961
                     /label=stem_loop
     misc_feature    2907..2986
                     /label=BBa_B0010
     terminator      2907..2986
                     /label=BBa_B0010
     stem_loop       3002..3021
                     /label=T7 TE
     misc_feature    3028..3028
                     /label=stop
     polyA_site      3022..3035
                     /label=polya
     misc_feature    2995..3035
                     /label=BBa_B0012
     terminator      2995..3035
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggact actagatgac catgattacc
      241 gtgcactaga ataccattaa cattgctaag aacgacttct ctgacatcga actggctgct
      301 atcccgttca acactctggc tgaccattac ggtgagcgtt tagctcgcga acagttggcc
      361 cttgagcatg agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt tgagcgtcaa
      421 cttaaagctg gtgaggttgc ggataacgct gccgccaagc ctctcatcac taccctactc
      481 cctaagatga ttgcacgcat caacgactgg tttgaggaag tgaaagctaa gcgcggcaag
      541 cgcccgacag ccttccagtt cctgtaggaa atcaagccgg aagccgtagc gtacatcacc
      601 attaagacca ctctggcttg cctaaccagt gctgacaata caaccgttca ggctgtagca
      661 agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg tgaccttgaa
      721 gctaagcact tcaagaaaaa cgttgaggaa caactcaaca agcgcgtagg gcacgtctac
      781 aagaaagcat ttatgcaagt tgtcgaggct gacatgctct ctaagggtct actcggtggc
      841 gaggcgtggt cttcgtggca taaggaagac tctattcatg taggagtacg ctgcatcgag
      901 atgctcattg agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt
      961 caagactctg agactatcga actcgcacct gaatacgctg aggctatcgc aacccgtgca
     1021 ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg tagttcctcc taagccgtgg
     1081 actggcatta ctggtggtgg ctattgggct aacggtcgtc gtcctctggc gctggtgcgt
     1141 actcacagta agaaagcact gatgcgctac gaagacgttt acatgcctga ggtgtacaaa
     1201 gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct agcggtcgcc
     1261 aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat tgagcgtgaa
     1321 gaactcccga tgaaaccgga agacatcgac atgaatcctg aggctctcac cgcgtggaaa
     1381 cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg tatcagcctt
     1441 gagttcatgc ttgagcaagc caataagttt gctaaccata aggccatctg gttcccttac
     1501 aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca aggtaacgat
     1561 atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga aggttactac
     1621 tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt ccctgagcgc
     1681 atcaagttca ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc tccactggag
     1741 aacacttggt gggctgagca agattctccg ttctgcttcc ttgcgttctg ctttgagtac
     1801 gctggggtac agcaccacgg cctgagctat aactgctccc ttccgctggc gtttgacggg
     1861 tcttgctctg gcatccagca cttctccgcg atgctccgag atgaggtagg tggtcgcgcg
     1921 gttaacttgc ttcctagtga aaccgttcag gacatctacg ggattgttgc taagaaagtc
     1981 aacgagattc tacaagcaga cgcaatcaat gggaccgata acgaagtagt taccgtgacc
     2041 gatgagaaca ctggtgaaat ctctgagaaa gtcaagctgg gcactaaggc actggctggt
     2101 caatggctgg cttacggtgt tactcgcagt gtgactaagc gttcagtcat gacgctggct
     2161 tacgggtcca aagagttcgg cttccgtcaa caagtgctgg aagataccat tcagccagct
     2221 attgattccg gcaagggtct gatgttcact cagccgaatc aggctgctgg atacatggct
     2281 aagctgattt gggaatctgt gagcgtgacg gtggtagctg cggttgaagc aatgaactgg
     2341 cttaagtctg ctgctaagct gctggctgct gaggtcaaag ataagaagac tggagagatt
     2401 cttcgcaagc gttgcgctgt gcattgggta actcctgatg gtttccctgt gtggcaggaa
     2461 tacaagaagc ctattcagac gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag
     2521 cctaccatta acaccaacaa agatagcgag attgatgcac acaaacagga gtctggtatc
     2581 gctcctaact ttgtacacag ccaagacggt agccaccttc gtaagactgt agtgtgggca
     2641 cacgagaagt acggaatcga atcttttgca ctgattcacg actccttcgg taccattccg
     2701 gctgacgctg cgaacctgtt caaagcagtg cgcgaaacta tggttgacac atatgagtct
     2761 tgtgatgtac tggctgattt ctacgaccag ttcgctgacc agttgcacga gtctcaattg
     2821 gacaaaatgc cagcacttcc ggctaaaggt aacttgaacc tccgtgacat cttagagtcg
     2881 gacttcgcgt tcgcataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     2941 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     3001 tggctcacct tcgggtgggc ctttctgcgt ttata
//