LOCUS       BBa_K228109             3038 bp    DNA     linear       17-May-2024
DEFINITION  LacP(R0010) + RBS(B0031) + T7ptag(K228000) + terminator(B0015)
ACCESSION   BBa_K228109
VERSION     BBa_K228109.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    215..218
                     /label=conserved
     RBS             209..222
                     /label=BBa_B0031
     CDS             229..2901
                     /label=cds
     CDS             229..2901
                     /label=BBa_K228000
     stem_loop       2921..2964
                     /label=stem_loop
     misc_feature    2910..2989
                     /label=BBa_B0010
     terminator      2910..2989
                     /label=BBa_B0010
     stem_loop       3005..3024
                     /label=T7 TE
     misc_feature    3031..3031
                     /label=stop
     polyA_site      3025..3038
                     /label=polya
     misc_feature    2998..3038
                     /label=BBa_B0012
     terminator      2998..3038
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat gaccatgatt
      241 accgtgcact agaataccat taacattgct aagaacgact tctctgacat cgaactggct
      301 gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg cgaacagttg
      361 gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat gtttgagcgt
      421 caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat cactacccta
      481 ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc taagcgcggc
      541 aagcgcccga cagccttcca gttcctgtag gaaatcaagc cggaagccgt agcgtacatc
      601 accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt tcaggctgta
      661 gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat ccgtgacctt
      721 gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt agggcacgtc
      781 tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg tctactcggt
      841 ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt acgctgcatc
      901 gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc tggcgtagta
      961 ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat cgcaacccgt
     1021 gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc tcctaagccg
     1081 tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct ggcgctggtg
     1141 cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc tgaggtgtac
     1201 aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt cctagcggtc
     1261 gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc gattgagcgt
     1321 gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct caccgcgtgg
     1381 aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc
     1441 cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat ctggttccct
     1501 tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac
     1561 gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa ggaaggttac
     1621 tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag
     1681 cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa gtctccactg
     1741 gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt ctgctttgag
     1801 tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct ggcgtttgac
     1861 gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc
     1921 gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt tgctaagaaa
     1981 gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt agttaccgtg
     2041 accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa ggcactggct
     2101 ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt catgacgctg
     2161 gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac cattcagcca
     2221 gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc tggatacatg
     2281 gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga agcaatgaac
     2341 tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa gactggagag
     2401 attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc tgtgtggcag
     2461 gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca gttccgctta
     2521 cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca ggagtctggt
     2581 atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg
     2641 gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt cggtaccatt
     2701 ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga cacatatgag
     2761 tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca cgagtctcaa
     2821 ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga catcttagag
     2881 tcggacttcg cgttcgcata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     2941 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     3001 cactggctca ccttcgggtg ggcctttctg cgtttata
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