LOCUS BBa_K228111 4604 bp DNA linear 23-May-2024 DEFINITION AND GATE AraC + SupD + LacP +RBS(J61107)+T7ptag ACCESSION BBa_K228111 VERSION BBa_K228111.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 misc_feature 1288..1560 /label=BBa_K228100 CDS 1569..1656 /label=end of LacI coding region (inactive) misc_feature 1569..1768 /label=BBa_R0010 misc_feature 1657..1694 /label=CAP binding site promoter 1705..1710 /label=-35 promoter 1729..1734 /label=-10 misc_feature 1734..1768 /label=LacI binding site misc_feature 1741..1741 /label=start promoter 1569..1768 /label=BBa_R0010 RBS 1777..1788 /label=BBa_J61107 CDS 1795..4467 /label=cds CDS 1795..4467 /label=BBa_K228000 stem_loop 4487..4530 /label=stem_loop misc_feature 4476..4555 /label=BBa_B0010 terminator 4476..4555 /label=BBa_B0010 stem_loop 4571..4590 /label=T7 TE misc_feature 4597..4597 /label=stop polyA_site 4591..4604 /label=polya misc_feature 4564..4604 /label=BBa_B0012 terminator 4564..4604 /label=BBa_B0012 misc_feature 1569..4604 /label=BBa_K228105 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 1621 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 1681 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 1741 aattgtgagc ggataacaat ttcacacata ctagagaaag aagagactta ctagatgacc 1801 atgattaccg tgcactagaa taccattaac attgctaaga acgacttctc tgacatcgaa 1861 ctggctgcta tcccgttcaa cactctggct gaccattacg gtgagcgttt agctcgcgaa 1921 cagttggccc ttgagcatga gtcttacgag atgggtgaag cacgcttccg caagatgttt 1981 gagcgtcaac ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc tctcatcact 2041 accctactcc ctaagatgat tgcacgcatc aacgactggt ttgaggaagt gaaagctaag 2101 cgcggcaagc gcccgacagc cttccagttc ctgtaggaaa tcaagccgga agccgtagcg 2161 tacatcacca ttaagaccac tctggcttgc ctaaccagtg ctgacaatac aaccgttcag 2221 gctgtagcaa gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt 2281 gaccttgaag ctaagcactt caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg 2341 cacgtctaca agaaagcatt tatgcaagtt gtcgaggctg acatgctctc taagggtcta 2401 ctcggtggcg aggcgtggtc ttcgtggcat aaggaagact ctattcatgt aggagtacgc 2461 tgcatcgaga tgctcattga gtcaaccgga atggttagct tacaccgcca aaatgctggc 2521 gtagtaggtc aagactctga gactatcgaa ctcgcacctg aatacgctga ggctatcgca 2581 acccgtgcag gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt agttcctcct 2641 aagccgtgga ctggcattac tggtggtggc tattgggcta acggtcgtcg tcctctggcg 2701 ctggtgcgta ctcacagtaa gaaagcactg atgcgctacg aagacgttta catgcctgag 2761 gtgtacaaag cgattaacat tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta 2821 gcggtcgcca acgtaatcac caagtggaag cattgtccgg tcgaggacat ccctgcgatt 2881 gagcgtgaag aactcccgat gaaaccggaa gacatcgaca tgaatcctga ggctctcacc 2941 gcgtggaaac gtgctgccgc tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt 3001 atcagccttg agttcatgct tgagcaagcc aataagtttg ctaaccataa ggccatctgg 3061 ttcccttaca acatggactg gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa 3121 ggtaacgata tgaccaaagg actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa 3181 ggttactact ggctgaaaat ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc 3241 cctgagcgca tcaagttcat tgaggaaaac cacgagaaca tcatggcttg cgctaagtct 3301 ccactggaga acacttggtg ggctgagcaa gattctccgt tctgcttcct tgcgttctgc 3361 tttgagtacg ctggggtaca gcaccacggc ctgagctata actgctccct tccgctggcg 3421 tttgacgggt cttgctctgg catccagcac ttctccgcga tgctccgaga tgaggtaggt 3481 ggtcgcgcgg ttaacttgct tcctagtgaa accgttcagg acatctacgg gattgttgct 3541 aagaaagtca acgagattct acaagcagac gcaatcaatg ggaccgataa cgaagtagtt 3601 accgtgaccg atgagaacac tggtgaaatc tctgagaaag tcaagctggg cactaaggca 3661 ctggctggtc aatggctggc ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg 3721 acgctggctt acgggtccaa agagttcggc ttccgtcaac aagtgctgga agataccatt 3781 cagccagcta ttgattccgg caagggtctg atgttcactc agccgaatca ggctgctgga 3841 tacatggcta agctgatttg ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca 3901 atgaactggc ttaagtctgc tgctaagctg ctggctgctg aggtcaaaga taagaagact 3961 ggagagattc ttcgcaagcg ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg 4021 tggcaggaat acaagaagcc tattcagacg cgcttgaacc tgatgttcct cggtcagttc 4081 cgcttacagc ctaccattaa caccaacaaa gatagcgaga ttgatgcaca caaacaggag 4141 tctggtatcg ctcctaactt tgtacacagc caagacggta gccaccttcg taagactgta 4201 gtgtgggcac acgagaagta cggaatcgaa tcttttgcac tgattcacga ctccttcggt 4261 accattccgg ctgacgctgc gaacctgttc aaagcagtgc gcgaaactat ggttgacaca 4321 tatgagtctt gtgatgtact ggctgatttc tacgaccagt tcgctgacca gttgcacgag 4381 tctcaattgg acaaaatgcc agcacttccg gctaaaggta acttgaacct ccgtgacatc 4441 ttagagtcgg acttcgcgtt cgcataatac tagagccagg catcaaataa aacgaaaggc 4501 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 4561 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //